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公开(公告)号：US11091766B2
公开(公告)日：2021-08-17
申请号：US15977265
申请日：2018-05-11
Applicant: Monsanto Technology LLC
Inventor： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
Abstract: Production of transformed plants is achieved by placing Agrobacterium-inoculated explants directly onto a medium containing selective agents for suppressing growth of Agrobacterium and killing non-transformed explant cells.
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2.发明申请公开(公告)号：US20180142308A1
公开(公告)日：2018-05-24
申请号：US15826538
申请日：2017-11-29
Applicant: Monsanto Technology, LLC
Inventor： Brenda A. Lowe , Paul S. Chomet
IPC: C12Q1/6895 , A01H1/00 , A01H5/10 , C12N15/82 , A01H1/04
CPC classification number: C12Q1/6895 , A01H1/00 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8241
Abstract: The current invention provides methods and compositions for producing an elite line of Zea mays enhanced for transformability. Compositions comprising an elite germplasm exhibiting increased transformability are disclosed. Further provided are methods for breeding maize such that enhanced transformability traits may be transferred to a desired germplasm. The plants and methods of the invention represent a valuable new tool for the creation of elite transgenic plants, preferably having one or more added beneficial characteristics.
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公开(公告)号：US11212972B2
公开(公告)日：2022-01-04
申请号：US16553911
申请日：2019-08-28
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
Abstract: The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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公开(公告)号：US10918032B2
公开(公告)日：2021-02-16
申请号：US16106342
申请日：2018-08-21
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US20170107527A1
公开(公告)日：2017-04-20
申请号：US15177576
申请日：2016-06-09
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Stanton B. Dotson , Larry A. Gilbertson , Jonathan C. Lamb , Brenda A. Lowe , Zoe P. McCuddin
IPC: C12N15/82
CPC classification number: C12N15/8213 , C12N15/8241 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8257 , C12N15/8261 , C12N15/827 , C12N15/8271 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N15/8289 , C12N15/829
Abstract: The invention provides novel uses of sequence-specific or sequence-directed endonucleases for molecular plant breeding. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of an endonuclease with plant expression and transformation elements. Plants and derivatives thereof produced by such methods are also provided.
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Patent Agency Ranking
公开(公告)号：US08962326B2
公开(公告)日：2015-02-24
申请号：US13945727
申请日：2013-07-18
Applicant: Monsanto Technology LLC
Inventor： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
CPC classification number: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
Abstract translation: 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。
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公开(公告)号：US08853488B2
公开(公告)日：2014-10-07
申请号：US13791186
申请日：2013-03-08
Applicant: Monsanto Technology LLC
Inventor： Jyoti R. Rout , Brenda A. Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
CPC classification number: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
Abstract translation: 本发明提供了通过简单且快速的方案转化单子叶植物的方法，以获得能够在4-8周内种植到土壤中的再生植物。 还提供了相关的细胞培养基和生长条件，以及通过该方法获得的植物和植物部分。 此外，还提供了通过本发明的方法筛选用于可变形性的顽固植物基因型的方法。 此外，还提供了通过本发明的方法扩展用于生产转基因植物的优先发展窗口的系统。
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公开(公告)号：US20140059717A1
公开(公告)日：2014-02-27
申请号：US13945727
申请日：2013-07-18
Applicant: Monsanto Technology LLC
Inventor： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
IPC: C12N15/82
CPC classification number: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
Abstract translation: 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。
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公开(公告)号：US20230099076A1
公开(公告)日：2023-03-30
申请号：US17955430
申请日：2022-09-28
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, JR. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
IPC: A01H4/00
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US20220010320A1
公开(公告)日：2022-01-13
申请号：US17370598
申请日：2021-07-08
Applicant: Monsanto Technology LLC
Inventor： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
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