公开(公告)号：US07214313B2

公开(公告)日：2007-05-08

申请号：US11088930

申请日：2005-03-25

Applicant: Morimasa Hayashi ,  Yosuke Iwata ,  Yusuke Osaka

Inventor： Morimasa Hayashi ,  Yosuke Iwata ,  Yusuke Osaka

IPC: B01D15/08

CPC classification number: G01N30/463 ,  G01N30/461 ,  G01N30/468 ,  G01N2030/085 ,  B01D15/361 ,  B01D15/325

Abstract: A two-dimensional liquid chromatograph includes a first-dimension separation channel for guiding a sample injected from a sample injection part to a first-dimension analysis column using a first-dimension analysis mobile phase for separation; two trap columns; an analysis channel for guiding components retained in the trap columns to a second-dimension analysis column using a second-dimension analysis mobile phase for analysis; and a channel switching mechanism. The switching mechanism connects the first-dimension separation channel to one of the trap columns and connecting the analysis channel to the other of the trap columns, and also switches connections between the trap columns and the channels.

Abstract translation: 二维液相色谱仪包括：第一维分离通道，用于使用第一维分析流动相分离从第一维分析柱引入从样品注入部分注入的样品; 两个陷阱列; 分析通道，用于使用二维分析流动相将捕集塔中保留的组分引导到二维分析柱进行分析; 和信道切换机制。 切换机构将第一维分离通道连接到其中一个陷阱列，并将分析通道连接到另一个陷阱列，还可以切换陷阱列和通道之间的连接。

公开(公告)号：US20050218055A1

公开(公告)日：2005-10-06

申请号：US11088930

申请日：2005-03-25

Applicant: Morimasa Hayashi ,  Yosuke Iwata ,  Yusuke Osaka

Inventor： Morimasa Hayashi ,  Yosuke Iwata ,  Yusuke Osaka

IPC: G01N30/46 ,  B01D15/08 ,  G01N30/08 ,  G01N30/26 ,  G01N30/72 ,  G01N30/74 ,  G01N30/78

CPC classification number: G01N30/463 ,  G01N30/461 ,  G01N30/468 ,  G01N2030/085 ,  B01D15/361 ,  B01D15/325

Abstract: A two-dimensional liquid chromatograph includes a first-dimension separation channel for guiding a sample injected from a sample injection part to a first-dimension analysis column using a first-dimension analysis mobile phase for separation; two trap columns; an analysis channel for guiding components retained in the trap columns to a second-dimension analysis column using a second-dimension analysis mobile phase for analysis; and a channel switching mechanism. The switching mechanism connects the first-dimension separation channel to one of the trap columns and connecting the analysis channel to the other of the trap columns, and also switches connections between the trap columns and the channels.

Abstract translation: 二维液相色谱仪包括：第一维分离通道，用于使用第一维分析流动相分离从第一维分析柱引入从样品注入部分注入的样品; 两个陷阱列; 分析通道，用于使用二维分析流动相将捕集塔中保留的组分引导到二维分析柱进行分析; 和信道切换机制。 切换机构将第一维分离通道连接到其中一个陷阱列，并将分析通道连接到另一个陷阱列，还可以切换陷阱列和通道之间的连接。

公开(公告)号：US20070178581A1

公开(公告)日：2007-08-02

申请号：US11607025

申请日：2006-12-01

Applicant: Yusuke Osaka ,  Morimasa Hayashi

Inventor： Yusuke Osaka ,  Morimasa Hayashi

IPC: G01N33/558 ,  C12M3/00

CPC classification number: G01N33/558

Abstract: An screening method and apparatus for online identification of a reactive compound bonding with a target compound includes a first dimension analysis step whereby a sample including a target compound and a reactive compound are injected together into a sample injection port. The sample is led to a first dimension column using a first dimension mobile phase and is separated into components. An isolation step isolates the sample portion into a loop passage. A reactive compound concentration step includes leading the reaction products into a trap column that selectively traps the reactive compound while dissociating it from the reaction products isolated by the isolation step. A second dimension analysis step leads the sample trapped in the trap column to a second dimension column using a second dimension mobile phase.

Abstract translation: 用于在线鉴定与目标化合物键合的反应性化合物的筛选方法和装置包括第一维度分析步骤，其中将包含目标化合物和反应性化合物的样品一起注入样品注入口。 使用第一维流动相将样品引导到第一维柱，并将其分成组分。 隔离步骤将样品部分隔离成环通道。 反应性化合物浓缩步骤包括引导反应产物进入捕集塔，该捕集塔选择性捕集反应性化合物，同时使其与通过分离步骤分离的反应产物解离。 第二维分析步骤使用第二维移动相将陷阱中捕获的样品引导到第二维列。

公开(公告)号：US20100162801A1

公开(公告)日：2010-07-01

申请号：US12278867

申请日：2006-02-09

Applicant: Morimasa Hayashi ,  Yoshihiro Hayakawa

Inventor： Morimasa Hayashi ,  Yoshihiro Hayakawa

IPC: G01N30/30

CPC classification number: G01N30/30 ,  G01N30/34 ,  G01N2030/3007 ,  G01N2030/347

Abstract: In order to obtain a temperature gradient elution method which is rapid and accurate, a mobile phase (1) is supplied through two mobile-phase flow channels (2a) and (2b), mixed together, and introduced into the column (15) while the column (15) is made adiabatic. In this operation, the temperature of the mobile phase in one mobile-phase flow channel, i.e., the channel (2a), is regulated to a constant temperature higher than the upper limit of the target temperature range to be obtained in the column (15), while the temperature of the mobile phase in the other mobile-phase flow channel, i.e., the channel (2b), is regulated to a constant temperature lower than the lower limit of the target temperature range in the column (15). By controlling the flow rates in the two mobile-phase flow channels (2a) and (2b), the proportion in which these mobile-phase portions are mixed is changed with time to thereby change the temperature of the mobile phase in the column (15) with time. The temperature gradient elution method is thus conducted.

Abstract translation: 为了获得快速准确的温度梯度洗脱方法，将流动相（1）通过两个流动相流动通道（2a）和（2b）供应，混合在一起，并引入塔（15），同时 柱（15）是绝热的。 在该操作中，一个流动相流路（即，通道（2a））中的流动相的温度被调节到高于在列（15）中获得的目标温度范围的上限的恒定温度 ），而另一流动相流路（即，通道（2b））中的流动相的温度被调节到低于列（15）中的目标温度范围的下限的恒定温度。 通过控制两个流动相流动通道（2a）和（2b）中的流速，混合这些流动相部分的比例随时间而改变，从而改变塔（15）中流动相的温度 ） 随着时间的推移。 因此进行温度梯度洗脱法。

公开(公告)号：US07526947B2

公开(公告)日：2009-05-05

申请号：US11360956

申请日：2006-02-23

Applicant: Nobuyuki Tatsumi ,  Morimasa Hayashi

Inventor： Nobuyuki Tatsumi ,  Morimasa Hayashi

IPC: G01N30/20 ,  G01N30/24

CPC classification number: G01N35/1097

Abstract: A function of automatically performing a pretreatment process on a sample is provided in an auto sampler of a total volume injection method that can realize high measurement accuracy even when an amount of the sample is small. A multi-port valve and a multi-position valve are connected to each other, and a plurality of kinds of cleaning solutions and also a mobile phase are connected to the multi-port valve. By providing a certain volume in a tube connected to a measuring pump for suctioning the sample, the pump can suction a sufficient amount of the cleaning solution or a diluent solution at one suction operation. Consequently, an apparatus according to the invention can deal with the pretreatment such as cleaning and dilution, without repeating the same suction/discharge operations.

Abstract translation: 在总体积注射方法的自动采样器中提供了在样品上自动执行预处理过程的功能，即使在样品量较小时也能实现高测量精度。 多口阀和多位置阀彼此连接，并且多种清洁溶液以及流动相连接到多口阀。 通过在与测量泵连接的管中提供一定体积以抽吸样品，泵可以在一次抽吸操作时吸入足够量的清洁溶液或稀释剂溶液。 因此，根据本发明的装置可以处理诸如清洁和稀释之类的预处理，而不必重复相同的抽吸/排出操作。

公开(公告)号：US08104330B2

公开(公告)日：2012-01-31

申请号：US12278867

申请日：2006-02-09

Applicant: Morimasa Hayashi ,  Yoshihiro Hayakawa

Inventor： Morimasa Hayashi ,  Yoshihiro Hayakawa

IPC: G01N30/30

CPC classification number: G01N30/30 ,  G01N30/34 ,  G01N2030/3007 ,  G01N2030/347

Abstract: In order to obtain a temperature gradient elution method which is rapid and accurate, a mobile phase (1) is supplied through two mobile-phase flow channels (2a) and (2b), mixed together, and introduced into the column (15) while the column (15) is made adiabatic. In this operation, the temperature of the mobile phase in one mobile-phase flow channel, i.e., the channel (2a), is regulated to a constant temperature higher than the upper limit of the target temperature range to be obtained in the column (15), while the temperature of the mobile phase in the other mobile-phase flow channel, i.e., the channel (2b), is regulated to a constant temperature lower than the lower limit of the target temperature range in the column (15). By controlling the flow rates in the two mobile-phase flow channels (2a) and (2b), the proportion in which these mobile-phase portions are mixed is changed with time to thereby change the temperature of the mobile phase in the column (15) with time. The temperature gradient elution method is thus conducted.

Abstract translation: 为了获得快速准确的温度梯度洗脱方法，将流动相（1）通过两个流动相流动通道（2a）和（2b）供应，混合在一起，并引入塔（15），同时 柱（15）是绝热的。 在该操作中，一个流动相流路（即，通道（2a））中的流动相的温度被调节到高于在列（15）中获得的目标温度范围的上限的恒定温度 ），而另一流动相流路（即，通道（2b））中的流动相的温度被调节到低于列（15）中的目标温度范围的下限的恒定温度。 通过控制两个流动相流动通道（2a）和（2b）中的流速，混合这些流动相部分的比例随时间而改变，从而改变塔（15）中流动相的温度 ） 随着时间的推移。 因此进行温度梯度洗脱法。

公开(公告)号：US20060196282A1

公开(公告)日：2006-09-07

申请号：US11360956

申请日：2006-02-23

Applicant: Nobuyuki Tatsumi ,  Morimasa Hayashi

Inventor： Nobuyuki Tatsumi ,  Morimasa Hayashi

IPC: G01N1/14

CPC classification number: G01N35/1097

Abstract: A function of automatically performing a pretreatment process on a sample is provided in an autosampler of a total volume injection method that can realize high measurement accuracy even when an amount of the sample is small. A multi-port valve and a multi-position valve are connected to each other, and a plurality of kinds of cleaning solutions and also a mobile phase are connected to the multi-port valve. By providing a certain volume in a tube connected to a measuring pump for suctioning the sample, the pump can suction a sufficient amount of the cleaning solution or a diluent solution at one suction operation. Consequently, an apparatus according to the invention can deal with the pretreatment such as cleaning and dilution, without repeating the same suction/discharge operations.

Abstract translation: 在总体积注射方法的自动进样器中提供了对样品进行自动执行预处理过程的功能，即使在样品量少的情况下也能够实现高测量精度。 多口阀和多位置阀彼此连接，并且多种清洁溶液以及流动相连接到多口阀。 通过在与测量泵连接的管中提供一定体积以抽吸样品，泵可以在一次抽吸操作时吸入足够量的清洁溶液或稀释剂溶液。 因此，根据本发明的装置可以处理诸如清洁和稀释之类的预处理，而不必重复相同的抽吸/排出操作。