-
公开(公告)号：US07943027B2
公开(公告)日：2011-05-17
申请号：US11979570
申请日：2007-11-06
申请人： Motohiro Yamazaki , Ryoji Inaba , Satoshi Takahashi , Tomohiro Shoji , Takeshi Ohura , Takashi Gomi
发明人： Motohiro Yamazaki , Ryoji Inaba , Satoshi Takahashi , Tomohiro Shoji , Takeshi Ohura , Takashi Gomi
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： B01D57/02 , C07K1/26 , G01N27/44721 , G01N27/44782
摘要： The object of the present invention is that a dynamic range is extended in an electrophoresis unit and concentration differences among a plurality of samples measured simultaneously are increased.An irradiation time to the samples is adjusted during analysis without changing a sampling time. By shortening the irradiation time, a fluorescence amount of the samples is reduced to cause signal intensity detected by a detector to physically decrease. If the irradiation time is very short (several 100 msec), the irradiation time and fluorescence intensity are in a direct proportional relationship. It is known that, if the irradiation time is reduced to 1/n, the fluorescence intensity, that is, signal intensity to be detected will be 1/n. Thus, for data whose irradiation time is reduced to 1/n during analysis, data obtained by multiplying a substantially measured value by n is used for data analysis as a true value to be originally acquired.
摘要翻译： 本发明的目的是在电泳单元中扩展动态范围，同时增加多个样本之间的浓度差。 在分析过程中调整样品的照射时间，而不改变采样时间。 通过缩短照射时间，减少样品的荧光量，使得由检测器检测出的信号强度物理降低。 如果照射时间非常短（几百毫秒），照射时间和荧光强度成正比关系。 已知如果照射时间减少到1 / n，则荧光强度即被检测信号强度为1 / n。 因此，对于在分析期间其照射时间减少到1 / n的数据，将通过将基本上测量的值乘以n获得的数据用作数据分析作为最初获取的真实值。
-
公开(公告)号：US20080110756A1
公开(公告)日：2008-05-15
申请号：US11979570
申请日：2007-11-06
申请人： Motohiro Yamazaki , Ryoji Inaba , Satoshi Takahashi , Tomohiro Shoji , Takeshi Ohura , Takashi Gomi
发明人： Motohiro Yamazaki , Ryoji Inaba , Satoshi Takahashi , Tomohiro Shoji , Takeshi Ohura , Takashi Gomi
IPC分类号： B01D57/02
CPC分类号： B01D57/02 , C07K1/26 , G01N27/44721 , G01N27/44782
摘要： The object of the present invention is that a dynamic range is extended in an electrophoresis unit and concentration differences among a plurality of samples measured simultaneously are increased.An irradiation time to the samples is adjusted during analysis without changing a sampling time. By shortening the irradiation time, a fluorescence amount of the samples is reduced to cause signal intensity detected by a detector to physically decrease. If the irradiation time is very short (several 100 msec), the irradiation time and fluorescence intensity are in a direct proportional relationship. It is known that, if the irradiation time is reduced to 1/n, the fluorescence intensity, that is, signal intensity to be detected will be 1/n. Thus, for data whose irradiation time is reduced to 1/n during analysis, data obtained by multiplying a substantially measured value by n is used for data analysis as a true value to be originally acquired.
摘要翻译： 本发明的目的是在电泳单元中扩展动态范围，同时增加多个样本之间的浓度差。 在分析过程中调整样品的照射时间，而不改变采样时间。 通过缩短照射时间，减少样品的荧光量，使得由检测器检测出的信号强度物理降低。 如果照射时间非常短（几百毫秒），照射时间和荧光强度成正比关系。 已知如果照射时间减少到1 / n，则荧光强度即被检测信号强度为1 / n。 因此，对于在分析期间照射时间减少到1 / n的数据，将通过将基本上测量的值乘以n获得的数据用作数据分析，作为最初获取的真实值。
-
公开(公告)号：US20080217177A1
公开(公告)日：2008-09-11
申请号：US12073296
申请日：2008-03-04
申请人： Ryoji Inaba , Motohiro Yamazaki , Tomohiro Shoji , Takeshi Ohura , Takashi Gomi
发明人： Ryoji Inaba , Motohiro Yamazaki , Tomohiro Shoji , Takeshi Ohura , Takashi Gomi
IPC分类号： G01N27/26
CPC分类号： G01N27/447
摘要： There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed.Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.
摘要翻译： 提供了一种毛细管电泳装置，其中激光照射光与毛细管阵列的耦合效率即使去除两个毛细管阵列的任何一个毛细管阵列也不会下降。 通过一个光学检测系统检测由一个辐射来自一侧的激发光的2μs（n是正整数）毛细管阵列的毛细管阵列的毛细管产生的发光。
-
公开(公告)号：US08262888B2
公开(公告)日：2012-09-11
申请号：US12073296
申请日：2008-03-04
申请人： Ryoji Inaba , Motohiro Yamazaki , Tomohiro Shoji , Takeshi Ohura , Takashi Gomi
发明人： Ryoji Inaba , Motohiro Yamazaki , Tomohiro Shoji , Takeshi Ohura , Takashi Gomi
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/447
摘要： There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed.Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.
摘要翻译： 提供了一种毛细管电泳装置，其中激光照射光与毛细管阵列的耦合效率即使去除两个毛细管阵列的任何一个毛细管阵列也不会下降。 通过一个光学检测系统检测由辐射来自一侧的激发光的2n（n是正整数）毛细管阵列的毛细管阵列的毛细管产生的发光。
-
公开(公告)号：US08702949B2
公开(公告)日：2014-04-22
申请号：US13263040
申请日：2010-04-08
申请人： Takeshi Ohura , Ryoji Inaba , Manabu Akiba , Takashi Gomi , Mari Kotoura
发明人： Takeshi Ohura , Ryoji Inaba , Manabu Akiba , Takashi Gomi , Mari Kotoura
IPC分类号： G01N27/453
CPC分类号： G01N27/44743 , G01N27/44782
摘要： The solution reservoir apparatus of the capillary electrophoresis apparatus securely affixes an evaporation-preventing membrane to a container when a capillary is inserted or withdrawn, without extending the cathode end of the capillary. The solution reservoir apparatus comprises a container for reserving a sample or solution, a cover having a bore through which the capillary is passed and covering the container, an evaporation-preventing membrane having a capillary hole through which the capillary is passed, and a container holder for holding the container. The evaporation-preventing membrane has a projection provided at the periphery of the capillary hole, the projection of the evaporation-preventing membrane is engaged with the bore on the cover when the evaporation-preventing membrane is positioned on the cover, and the evaporation-preventing membrane is supported by the cover.
摘要翻译： 当毛细管插入或取出时，毛细管电泳装置的溶液储存装置在不延长毛细管的阴极端的情况下，将防蒸发膜牢固地固定在容器上。 溶液储存装置包括用于储存样品或溶液的容器，具有孔的盖，毛细管穿过孔覆盖容器，具有毛细孔通过的毛细孔的防蒸发膜和容器保持架 用于拿着容器。 防蒸发膜具有设置在毛细孔周边的突起，当防蒸发膜位于盖上时，防蒸发膜的突起与盖上的孔接合，并且防蒸发膜 膜由盖支撑。
-
公开(公告)号：US20120031762A1
公开(公告)日：2012-02-09
申请号：US13263304
申请日：2010-04-06
申请人： Takashi Gomi , Ryoji Inaba , Taro Nakazawa , Takeshi Ohura , Mari Kotoura
发明人： Takashi Gomi , Ryoji Inaba , Taro Nakazawa , Takeshi Ohura , Mari Kotoura
CPC分类号： F04B19/006 , B01D57/02 , G01N27/44704
摘要： Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.
摘要翻译： 不管泵的个体差异，将电泳介质填充到毛细管中，都可以去除气泡。 在容纳电泳介质的容器的内侧面和柱塞的侧面之间形成的流路之间形成有引起电泳介质容易停滞的流路之一，其横截面积大于 另一个流动通道的相对侧的横截面面积。 换句话说，导致电泳介质容易停滞的流通部分以增加电泳介质的流量的方式形成。 这可以消除在泵中具有极少量的电泳介质流动的区域。
-
公开(公告)号：US08349161B2
公开(公告)日：2013-01-08
申请号：US13263304
申请日：2010-04-06
申请人： Takashi Gomi , Ryoji Inaba , Taro Nakazawa , Takeshi Ohura , Mari Kotoura
发明人： Takashi Gomi , Ryoji Inaba , Taro Nakazawa , Takeshi Ohura , Mari Kotoura
IPC分类号： G01N27/453
CPC分类号： F04B19/006 , B01D57/02 , G01N27/44704
摘要： Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.
摘要翻译： 不管泵的个体差异，将电泳介质填充到毛细管中，都可以去除气泡。 在容纳电泳介质的容器的内侧面和柱塞的侧面之间形成的流路之间形成有使电泳介质容易停滞的流路之一，其横截面积大于 另一个流动通道的相对侧的横截面面积。 换句话说，导致电泳介质容易停滞的流通部分以增加电泳介质的流量的方式形成。 这可以消除在泵中具有极少量的电泳介质流动的区域。
-
公开(公告)号：US20120024707A1
公开(公告)日：2012-02-02
申请号：US13263040
申请日：2010-04-08
申请人： Takeshi Ohura , Ryoji Inaba , Manabu Akiba , Takashi Gomi , Mari Kotoura
发明人： Takeshi Ohura , Ryoji Inaba , Manabu Akiba , Takashi Gomi , Mari Kotoura
IPC分类号： G01N27/453
CPC分类号： G01N27/44743 , G01N27/44782
摘要： The solution reservoir apparatus of the capillary electrophoresis apparatus securely affixes an evaporation-preventing membrane to a container when a capillary is inserted or withdrawn, without extending the cathode end of the capillary. The solution reservoir apparatus comprises a container for reserving a sample or solution, a cover having a bore through which the capillary is passed and covering the container, an evaporation-preventing membrane having a capillary hole through which the capillary is passed, and a container holder for holding the container. The evaporation-preventing membrane has a projection provided at the periphery of the capillary hole, the projection of the evaporation-preventing membrane is engaged with the bore on the cover when the evaporation-preventing membrane is positioned on the cover, and the evaporation-preventing membrane is supported by the cover.
摘要翻译： 当毛细管插入或取出时，毛细管电泳装置的溶液储存装置在不延长毛细管的阴极端的情况下，将防蒸发膜牢固地固定在容器上。 溶液储存装置包括用于储存样品或溶液的容器，具有孔的盖，毛细管穿过孔覆盖容器，具有毛细孔通过的毛细孔的防蒸发膜和容器保持架 用于拿着容器。 防蒸发膜具有设置在毛细孔周边的突起，当防蒸发膜位于盖上时，防蒸发膜的突起与盖上的孔接合，并且防蒸发膜 膜由盖支撑。
-
公开(公告)号：US20090211911A1
公开(公告)日：2009-08-27
申请号：US12344053
申请日：2008-12-24
申请人： Takeshi Ohura , Ryoji Inaba , Yoshitaka Kodama , Hiromi Yamashita , Manabu Akiba , Tomohiro Shoji
发明人： Takeshi Ohura , Ryoji Inaba , Yoshitaka Kodama , Hiromi Yamashita , Manabu Akiba , Tomohiro Shoji
IPC分类号： G01N27/26
CPC分类号： G01N35/00732 , G01N27/44743 , G01N2035/00316
摘要： The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.
摘要翻译： 本发明提供一种可以提高可操作性和测量速度的毛细管电泳装置。 根据本发明，用于识别样品容器类型的传感器固定在远离毛细管阳极电极的位置。 通过移动移动台使传感器更靠近样品容器，使得可以通过传感器识别设置在移动台上的样品容器。 用于固定至少一对样本容器的定影装置设置在移动台上。
-
公开(公告)号：US08685220B2
公开(公告)日：2014-04-01
申请号：US13559445
申请日：2012-07-26
申请人： Takeshi Ohura , Ryoji Inaba , Yoshitaka Kodama , Hiromi Yamashita , Manabu Akiba , Tomohiro Shoji
发明人： Takeshi Ohura , Ryoji Inaba , Yoshitaka Kodama , Hiromi Yamashita , Manabu Akiba , Tomohiro Shoji
CPC分类号： G01N35/00732 , G01N27/44743 , G01N2035/00316
摘要： The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.
摘要翻译： 本发明提供一种可以提高可操作性和测量速度的毛细管电泳装置。 根据本发明，用于识别样品容器类型的传感器固定在远离毛细管阳极电极的位置。 通过移动移动台使传感器更靠近样品容器，使得可以通过传感器识别设置在移动台上的样品容器。 用于固定至少一对样本容器的定影装置设置在移动台上。
-
-
-
-
-
-
-
-
-
搜索结果
79 条记录 (耗时 0.025 秒)