公开(公告)号：US10077430B2

公开(公告)日：2018-09-18

申请号：US14646730

申请日：2013-11-21

申请人： NATIONAL CANCER CENTER

发明人： Sang Jin Lee ,  Yun-Hee Kim ,  In-Hoo Kim ,  Seong-Wook Lee ,  Jin-Sook Jeong ,  Sang Young Han

IPC分类号： C12N15/861 ,  C12N15/113 ,  C12N15/33 ,  C12N15/34 ,  C12N15/52 ,  A61K48/00 ,  C12N7/00 ,  A61K35/761 ,  A61K38/45 ,  A61K31/713 ,  G01N33/574 ,  G01N33/569 ,  C12N15/86

CPC分类号： C12N7/00 ,  A61K31/713 ,  A61K35/761 ,  A61K38/45 ,  A61K48/0066 ,  C12N15/1135 ,  C12N15/86 ,  C12N2310/1241 ,  C12N2320/32 ,  C12N2330/51 ,  C12N2710/10021 ,  C12N2710/10032 ,  C12N2710/10343 ,  C12N2710/16633 ,  C12N2810/6018 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2840/445 ,  C12Y207/01021 ,  C12Y207/07049 ,  G01N33/56983 ,  G01N33/57438 ,  G01N2333/075

摘要： The present invention relates to a recombinant adenovirus with increased in-vivo safety, tissue specificity, and anticancer activities, and a use thereof. Specifically, the recombinant adenovirus comprising: a promoter of the liver tissue-specific phosphoenolpyruvate carboxykinase (PEPCK) gene; a trans-splicing ribozyme which is operably linked to the promoter and acts on a cancer-specific gene; a therapeutic gene or a reporter gene which is linked to the 3′ exon of the ribozyme; and a serotype 35 fiber knob and a serotype 5 shaft, in which the orf4 gene is deleted from adenovirus E1, E3 and E4 orf1, shows remarkable in-vivo safety, high specificity for a target tissue, and remarkable anticancer effects, and thus can be useful for an anticancer drug or a cancer diagnostic agent as a gene delivery vector.