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公开(公告)号：US11781181B2
公开(公告)日：2023-10-10
申请号：US16782794
申请日：2020-02-05
申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , Kyorin Pharmaceutical Co., Ltd.
CPC分类号： C12Q1/686 , B01L3/502715 , B01L7/525 , C12M1/00 , C12N15/09 , C12Q1/6851 , B01L2300/0654 , B01L2300/0883 , B01L2400/0475 , Y02A40/70
摘要： The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising:
heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone;
a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones;
a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.-
公开(公告)号：US11098347B2
公开(公告)日：2021-08-24
申请号：US15322000
申请日：2015-07-07
申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , Kyorin Pharmaceutical Co., Ltd.
摘要： The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising:
heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone;
a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones;
a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.-
3.发明授权公开(公告)号：US12032286B2
公开(公告)日：2024-07-09
申请号：US16902962
申请日：2020-06-16
申请人： ASAHI KASEI KABUSHIKI KAISHA , NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
CPC分类号： G03F7/0035 , B01L3/502707 , B81C1/00071 , G03F7/094 , G03F7/32 , B81C2201/0159
摘要： Provided is a method for producing a multi-layered microchannel device by using a photosensitive resin laminate, which is highly-defined and excellent in dimension accuracy and enables channels to be partially hydrophilized or hydrophobilized, wherein the method comprises step (i) of sequentially carrying out (i-a) forming a first photosensitive resin layer on a substrate, (i-b) light-exposing the first photosensitive resin layer, and (i-c) developing the light-exposed photosensitive layer and forming a channel pattern layer, to form a first channel pattern layer; and step (ii) of sequentially carrying out (ii-a) laminating a second photosensitive resin laminate on the first channel pattern layer formed in the step (i), (ii-b) light-exposing a photosensitive layer of the second photosensitive resin laminate, and (ii-c) developing the light-exposed photosensitive layer and forming a channel pattern layer, to form a second channel pattern layer.
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4.发明申请公开(公告)号：US20230053900A1
公开(公告)日：2023-02-23
申请号：US17980153
申请日：2022-11-03
申请人： ASAHI KASEI KABUSHIKI KAISHA , NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
摘要： Provided is a method for producing a multi-layered microchannel device by using a photosensitive resin laminate, which is highly-defined and excellent in dimension accuracy and enables channels to be partially hydrophilized or hydrophobilized, wherein the method comprises step (i) of sequentially carrying out (i-a) forming a first photosensitive resin layer on a substrate, (i-b) light-exposing the first photosensitive resin layer, and (i-c) developing the light-exposed photosensitive layer and forming a channel pattern layer, to form a first channel pattern layer; and step (ii) of sequentially carrying out (ii-a) laminating a second photosensitive resin laminate on the first channel pattern layer formed in the step (i), (ii-b) light-exposing a photosensitive layer of the second photosensitive resin laminate, and (ii-c) developing the light-exposed photosensitive layer and forming a channel pattern layer, to form a second channel pattern layer.
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