公开(公告)号：US10222599B2

公开(公告)日：2019-03-05

申请号：US14327189

申请日：2014-07-09

申请人： NIKON CORPORATION

发明人： Yumiko Ouchi ,  Kazuhiro Takasago ,  Hiroaki Nakayama

IPC分类号： G02B21/06 ,  G02B21/00 ,  G02B21/36 ,  G02B27/58 ,  G01N21/64

摘要： A structured illuminating apparatus includes a branching unit branching an exit light flux from a light source into at least two branched light fluxes, an illuminating optical system making the two branched light fluxes to be respectively collected at mutually different positions on a pupil plane of an objective lens and making the two branched light fluxes to be interfered with each other to illuminate a specimen with an interference fringe of the two branched light fluxes, and an adjusting unit adjusting a height from an optical axis of the illuminating optical system to two collecting points formed on the pupil plane of the objective lens by the two branched light fluxes.

公开(公告)号：US09897790B2

公开(公告)日：2018-02-20

申请号：US14996717

申请日：2016-01-15

申请人： NIKON CORPORATION

发明人： Hiroaki Nakayama ,  Yumiko Ouchi

IPC分类号： F21V9/16 ,  G02B21/06 ,  G01N21/64 ,  G02B21/00 ,  G02B21/36 ,  G02B27/58 ,  G02B21/16

CPC分类号： G02B21/06 ,  G01N21/64 ,  G01N21/6428 ,  G01N2021/6439 ,  G01N2201/063 ,  G01N2201/0638 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： A structured illumination device includes: a diffraction unit that diffracts light beams of a plurality of wavelengths that are emitted simultaneously or sequentially by a light source into a plurality of diffracted beams; and an optical system that forms interference fringes on a surface of a sample using the plurality of diffracted beams diffracted by the diffraction unit, the optical system including a first optical system and a second optical system that focuses the plurality of diffracted beams at positions on or near a pupil plane of the first optical system, and a magnification characteristic dY(λ) of the second optical system satisfying the condition of (fo·nw−afλ/P)≦dY(λ)≦(fo·NA−afλ/P), where a=1 (for M=1, 2) or a=2 (for M=3).

公开(公告)号：US10241314B2

公开(公告)日：2019-03-26

申请号：US14989106

申请日：2016-01-06

申请人： NIKON CORPORATION

发明人： Yumiko Ouchi ,  Takashi Kawahito

IPC分类号： G02B21/36 ,  G02B7/00 ,  G02B21/16 ,  G02B27/00 ,  G02B21/02 ,  G06T5/50

摘要： A microscope apparatus includes a monitoring optical system, an imaging unit capturing an image of an observation target through the monitoring optical system to generate a plurality of images, a correction unit disposed in the optical monitoring system and correcting various aberrations which occur due to an observation condition, and a decision unit deciding a correction amount of the correction unit based on the plurality of images generated by the imaging unit, whereby an image deterioration ascribable to the aberration occurring due to the observation condition in the microscope apparatus is appropriately and easily corrected according to a use condition of the microscope apparatus.

公开(公告)号：US10802259B2

公开(公告)日：2020-10-13

申请号：US16249467

申请日：2019-01-16

申请人： NIKON CORPORATION

发明人： Yumiko Ouchi ,  Kazuhiro Takasago ,  Hiroaki Nakayama

IPC分类号： G02B21/06 ,  G01N21/64 ,  G01B21/00 ,  G02B21/36 ,  G02B27/58 ,  G02B21/00

摘要： A structured illuminating apparatus includes a branching unit branching an exit light flux from a light source into at least two branched light fluxes, an illuminating optical system making the two branched light fluxes to be respectively collected at mutually different positions on a pupil plane of an objective lens and making the two branched light fluxes to be interfered with each other to illuminate a specimen with an interference fringe of the two branched light fluxes, and an adjusting unit adjusting a height from an optical axis of the illuminating optical system to two collecting points formed on the pupil plane of the objective lens by the two branched light fluxes.

公开(公告)号：US08773758B2

公开(公告)日：2014-07-08

申请号：US13687347

申请日：2012-11-28

申请人： Nikon Corporation

发明人： Hiroaki Nakayama ,  Yumiko Ouchi

IPC分类号： G02B21/02

CPC分类号： G02B21/02 ,  G01N21/6458 ,  G02B21/16 ,  G02B21/361 ,  G02B21/367 ,  G02B27/58

摘要： There is provided a microscope apparatus including: a plurality of objective lenses having different magnifications; an imaging system that receives light, which is generated from a sample and emitted from the objective lens when excitation light is emitted to a sample including a fluorescent material that is activated when irradiated with activation light having a predetermined wavelength and fluoresces to be inactivated when irradiated with excitation light having a different wavelength from the activation light in the activation state and that images the light in a state where an astigmatic difference is given to the image of the sample; and an imaging device that captures the image of the sample from the imaging system. The imaging system includes an astigmatic difference changing device that changes the astigmatic difference according to the depth of focus of the objective lens.

摘要翻译： 提供了一种显微镜装置，包括：具有不同倍率的多个物镜; 一种成像系统，其接收从样品产生并且当激发光发射到包含当照射具有预定波长的活化光时被激活的荧光材料的样品的物体发射的光，并且在照射时发荧光被灭活 激发光具有与激活状态下的激活光不同波长的激发光，并且在对样本的图像给出像散差异的状态下对光进行成像; 以及从成像系统捕获样品的图像的成像装置。 成像系统包括根据物镜的焦深改变散光差异的散光差异改变装置。

公开(公告)号：US20130314776A1

公开(公告)日：2013-11-28

申请号：US13909456

申请日：2013-06-04

申请人： NIKON CORPORATION

发明人： Masahiro MIZUTA ,  Hiroaki Nakayama ,  Yumiko Ouchi

IPC分类号： G02B15/14 ,  G02B21/22 ,  G02B21/02

CPC分类号： G02B15/14 ,  G02B21/025 ,  G02B21/082 ,  G02B21/22

摘要： A variable power optical system used in a parallel stereoscopic microscope apparatus includes a plurality of optical paths in which optical axes are arranged substantially parallel, a plurality of lens groups that change the magnification of a diameter of a luminous flux entering substantially parallel to each of the optical paths to eject the luminous flux as substantially parallel luminous fluxes, and at least two lens groups move along the optical axis in each optical path according to the change in the magnification. At least two lens groups of at least one optical path among the plurality of optical paths move in a direction including a component perpendicular to the optical axis according to the change in the magnification at at least part of a section where the magnification is changed from a high-power end state to a low-power end.

摘要翻译： 在并行立体显微镜装置中使用的可变功率光学系统包括光轴基本上平行布置的多个光路，多个透镜组，其改变基本上平行于每一个的光束的直径的倍率 用于将光通量作为基本上平行的光束喷射的光路，并且至少两个透镜组根据放大率的变化沿着每个光路中的光轴移动。 多个光路中的至少一个光路的至少两个透镜组在包括垂直于光轴的分量的方向上移动，根据放大率的变化在放大率从 大功率终端状态到低功耗端。

公开(公告)号：US10558030B2

公开(公告)日：2020-02-11

申请号：US15832203

申请日：2017-12-05

申请人： NIKON CORPORATION

发明人： Yumiko Ouchi

IPC分类号： H04N7/18 ,  G02B21/36 ,  G01N21/64 ,  G02B21/00 ,  G02B21/08 ,  G02B21/16 ,  G02B27/58

摘要： A structured illumination microscopy system including an illumination optical system illuminating excitation light to excite a fluorescent material contained in a sample on the sample with an interference fringe; a controlling part controlling a direction, a phase, and a spatial frequency of the interference fringe; an image-forming optical system forming an image of the sample which is modulated by illumination of the interference fringe; an imaging sensor capturing the image formed by the image-forming optical system; and a demodulating part performing demodulation processing by using a plurality of images captured by the imaging sensor in which the controlling part controls the spatial frequency of the interference fringe in accordance with an illuminating position of the interference fringe.

公开(公告)号：US10067328B2

公开(公告)日：2018-09-04

申请号：US14132777

申请日：2013-12-18

申请人： NIKON CORPORATION

发明人： Yumiko Ouchi

IPC分类号： G02B21/36 ,  G02B21/00 ,  G02B21/14 ,  G02B21/16 ,  H04N5/225

CPC分类号： G02B21/0092 ,  G02B6/2706 ,  G02B6/2848 ,  G02B21/14 ,  G02B21/16 ,  G02B21/367 ,  H04N5/2256

摘要： An illumination optical system includes: a beam splitter located near a conjugate position of a specimen and configured to split beams from a light source into a plurality of groups of beams having different splitting directions around an optical axis; a beam selector configured to select and transmit one group of beams from the plurality of groups of beams and that is rotatable with respect to the optical axis; and a ½ wavelength plate located near the beam selector and rotatable about the optical axis. The rotation angles of the ½ wavelength plate and of the beam selector about the optical axis are respectively set so that the polarization direction of the beam which has passed through the ½ wavelength plate is perpendicular to the splitting direction of the one group of beams that has been selected by the beam selector and split by the beam splitter.

公开(公告)号：US20160116727A1

公开(公告)日：2016-04-28

申请号：US14989106

申请日：2016-01-06

申请人： NIKON CORPORATION

发明人： Yumiko Ouchi ,  Takashi Kawahito

IPC分类号： G02B21/36 ,  G02B21/02 ,  G02B27/00 ,  G06T5/50

CPC分类号： G02B21/365 ,  G02B7/008 ,  G02B21/02 ,  G02B21/16 ,  G02B27/0068 ,  G02B2207/113 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/10148

摘要： A microscope apparatus includes a monitoring optical system, an imaging unit capturing an image of an observation target through the monitoring optical system to generate a plurality of images, a correction unit disposed in the optical monitoring system and correcting various aberrations which occur due to an observation condition, and a decision unit deciding a correction amount of the correction unit based on the plurality of images generated by the imaging unit, whereby an image deterioration ascribable to the aberration occurring due to the observation condition in the microscope apparatus is appropriately and easily corrected according to a use condition of the microscope apparatus.

摘要翻译： 显微镜装置包括监视光学系统，成像单元，通过监视光学系统捕获观察目标的图像以产生多个图像;校正单元，设置在光学监视系统中，并校正由于观察而发生的各种像差 条件，以及决定单元，基于由所述成像单元生成的所述多个图像来确定所述校正单元的校正量，从而根据所述显微镜装置中的观察条件而产生的产生的像差的图像劣化可以根据 到显微镜装置的使用条件。

公开(公告)号：US11988822B2

公开(公告)日：2024-05-21

申请号：US16806101

申请日：2020-03-02

申请人： NIKON CORPORATION

发明人： Toru Murayama ,  Atsushi Takeuchi ,  Yumiko Ouchi ,  Yuki Yoshida

IPC分类号： G02B21/06 ,  G02B21/00 ,  G02B21/24 ,  G02B21/36

CPC分类号： G02B21/0088 ,  G02B21/24 ,  G02B21/361

摘要： The present invention relates to a microscope of which visibility, controllability and operability can be improved. In the microscope, an optical path and optical path of an image forming system are set so as to be perpendicular to each other when viewed from the top. In other words, in this microscope, there exists an ocular optical system that guides light, which propagates the optical path to optical path of the image forming system, to a user. The optical path is formed in a direction perpendicular to a direction of the light from a sample emitted from the ocular optical system to the user. The present invention can be applied to an inverted microscope.