公开(公告)号：US20170044209A1

公开(公告)日：2017-02-16

申请号：US15306941

申请日：2015-04-30

Applicant: NOVOZYMES A/S

Inventor： Cedric Hobel ,  Heidi Winterberg Andersen ,  Peter Frode Pind ,  Cecilia Jansson Kepka

IPC: C07K1/14 ,  C12N9/82 ,  C12N15/10 ,  C12N9/26

CPC classification number: C07K1/14 ,  C07K1/145 ,  C12N9/2414 ,  C12N9/82 ,  C12N15/1003 ,  C12Y302/01001 ,  C12Y305/01001

Abstract: A method for reducing the level of DNA in a fermentation broth is disclosed where the method includes a heating step where the broth is heated to a temperature of at least 70° C.

Abstract translation: 公开了一种用于降低发酵液中DNA水平的方法，其中该方法包括加热步骤，其中将肉汤加热至至少70℃

公开(公告)号：US20180030087A1

公开(公告)日：2018-02-01

申请号：US15782282

申请日：2017-10-12

Applicant: NOVOZYMES A/S

Inventor： Cedric Hobel ,  Cecilia Jansson Kepka

IPC: C07K1/30 ,  C12N15/10 ,  C07K1/14

CPC classification number: C07K1/30 ,  C07K1/14 ,  C12N15/1003

Abstract: A method for removing residual DNA from a fermentation broth comprising a protein of interest and a microorganism producing the protein of interest, said method comprising: a) adding a poly aluminium chloride to the fermentation broth, and b) separating the flocculated microorganism from the fermentation broth.

公开(公告)号：US09676817B2

公开(公告)日：2017-06-13

申请号：US14364928

申请日：2012-12-19

Applicant: Novozymes A/S

Inventor： Poul Johansen ,  Cecilia Jansson Kepka

IPC: C07K1/36 ,  C07K1/30 ,  A61L2/00 ,  C07K1/00

CPC classification number: C07K1/30 ,  A61L2/0011

Abstract: The author attempted and succeeded in separating and purifying the cysteine protease inhibitors from new gingers. The root and stem of new gingers were extracted with neutral buffer which subsequently underwent heat treatment at 80° C. for 10 minutes. After centrifugal separation, the supernatant was separated with acetone. The fractions that precipitated at an acetone concentration of 55-77% were injected into a chromatographic column of DEAE-cellulose and Sephadex G-75 gel for purification. The results demonstrated that there were two cysteine protease inhibitors with a molecular weight of 11,000-11,800 and 15,500-16,000, respectively, which strongly inhibit papain. The two cysteine protease inhibitors do not have high heat stability.

公开(公告)号：US20150291656A1

公开(公告)日：2015-10-15

申请号：US14438766

申请日：2013-11-01

Applicant: NOVOZYMES A/S

Inventor： Cedric Hobel ,  Cecilia Jansson Kepka

IPC: C07K1/30

CPC classification number: C07K1/30 ,  C07K1/14 ,  C12N15/1003

Abstract: A method for removing residual DNA from a fermentation broth comprising a protein of interest and a microorganism producing the protein of interest, said method comprising: a) adding a poly aluminium chloride to the fermentation broth, and b) separating the flocculated microorganism from the fermentation broth.

Abstract translation: 一种从包含感兴趣的蛋白质和产生目标蛋白质的微生物的发酵液中除去残留DNA的方法，所述方法包括：a）向发酵液中加入聚氯化铝，和b）将絮凝的微生物与发酵物分离 肉汤。

公开(公告)号：US10259841B2

公开(公告)日：2019-04-16

申请号：US15306941

申请日：2015-04-30

Applicant: NOVOZYMES A/S

Inventor： Cedric Hobel ,  Heidi Winterberg Andersen ,  Peter Frode Pind ,  Cecilia Jansson Kepka

IPC: C12N9/26 ,  C12Q1/68 ,  C07K1/14 ,  C12N9/82 ,  C12N15/10

Abstract: A method for reducing the level of DNA in a fermentation broth is disclosed where the method includes a heating step where the broth is heated to a temperature of at least 70° C.

公开(公告)号：US20140335594A1

公开(公告)日：2014-11-13

申请号：US14364928

申请日：2012-12-19

Applicant: Novozymes A/S

Inventor： Poul Johansen ,  Cecilia Jansson Kepka

IPC: C07K1/30

CPC classification number: C07K1/30 ,  A61L2/0011

Abstract: The present invention relates to a method for selectively removing virus and/or virus DNA from a solution comprising a target protein, whereby acetone is used as a precipitation agent to precipitate virus and/or virus DNA as well as the target protein.

Abstract translation: 本发明涉及一种从包含靶蛋白的溶液中选择性除去病毒和/或病毒DNA的方法，其中使用丙酮作为沉淀剂沉淀病毒和/或病毒DNA以及靶蛋白。