公开(公告)号：US20060073470A1

公开(公告)日：2006-04-06

申请号：US10515994

申请日：2003-05-28

申请人： Naohiro Noda ,  Mutsuhisa Hiraoka ,  Kazuhito Takahashi ,  Koji Maruyama ,  Takeshi Saika ,  Yasunobu Tanaka ,  Masao Nasu ,  Nobuyasu Yamaguchi

发明人： Naohiro Noda ,  Mutsuhisa Hiraoka ,  Kazuhito Takahashi ,  Koji Maruyama ,  Takeshi Saika ,  Yasunobu Tanaka ,  Masao Nasu ,  Nobuyasu Yamaguchi

IPC分类号： C12Q1/00 ,  C12Q1/04

CPC分类号： G01N21/6456 ,  C12Q1/04 ,  G01N21/6428 ,  G01N2021/6439

摘要： A method of counting microorganisms or cells in a sample by labeling the microorganisms or cells with a fluorescent labeling reagent, which comprises contacting the sample containing the microorganisms or cells with an adhesive sheet having an adhesive layer laminated on at least one surface of a substrate to capture the sample; before fluorescent-labeling the microorganisms or cells, obtaining a first fluorescent image of the sample; after fluorescent-labeling the microorganisms or the cells, obtaining a second fluorescent image of the sample, and then determining the difference in the number of luminous points between the first and second images, or determining a differential image between the first and second images and determining the number of luminous points in this differential image, or determining the number of luminous points among the luminous points in the second image located outside non-sensitive regions attached to the individual luminous points in the first image.

摘要翻译： 一种通过用荧光标记试剂标记微生物或细胞来计数样品中的微生物或细胞的方法，该方法包括将含有微生物或细胞的样品与层压在基材的至少一个表面上的粘合剂层的粘合片接触到 捕获样品; 在对微生物或细胞进行荧光标记之前，获得样品的第一荧光图像; 在对微生物或细胞进行荧光标记之后，获得样品的第二荧光图像，然后确定第一和第二图像之间的发光点数量的差异，或者确定第一和第二图像之间的差分图像，并确定 该差分图像中的发光点的数量，或者确定位于附加到第一图像中的各个发光点的非敏感区域之外的第二图像中的发光点中的发光点的数量。

公开(公告)号：US20050208295A1

公开(公告)日：2005-09-22

申请号：US10526794

申请日：2003-09-03

申请人： Takeshi Saika ,  Koji Maruyama ,  Yasunobu Tanaka ,  Naohiro Noda ,  Takuya Onodera ,  Masao Nasu ,  Nobuyasu Yamaguchi

发明人： Takeshi Saika ,  Koji Maruyama ,  Yasunobu Tanaka ,  Naohiro Noda ,  Takuya Onodera ,  Masao Nasu ,  Nobuyasu Yamaguchi

IPC分类号： C12M1/26 ,  C12Q1/06 ,  B32B7/12 ,  B32B15/04

CPC分类号： C12Q1/06 ,  Y10T428/28 ,  Y10T428/2848

摘要： The present invention provides an adhesive sheet and a kit for microbial testing, which enable monitoring of the presence of a microorganism and/or the cell count thereof on a solid surface conveniently on a real time basis, and which accommodate to automated focusing during image analysis. The present invention relates to an adhesive sheet for microbial testing having at least a substrate and an adhesive layer, which adhesive layer is compression-bonded to, and peeled from, the surface of a test article to collect microorganisms, and the surface of the adhesive layer is then subjected to image analysis, wherein a marker for focusing the image is provided in the substrate, or in the adhesive layer, or on the surface thereof.

摘要翻译： 本发明提供一种用于微生物测试的粘合片和用于微生物测试的试剂盒，其能够在实时基础上方便地监测微生物的存在和/或其细胞计数，并且其可适应于图像分析期间的自动聚焦 。 本发明涉及一种用于微生物测试的粘合片，其至少具有基材和粘合剂层，该粘合剂层被压制粘合并从测试物品的表面上剥离以收集微生物，并且粘合剂的表面 然后对层进行图像分析，其中用于聚焦图像的标记设置在基板中，或者在粘合剂层中或其表面上。

公开(公告)号：US20060148028A1

公开(公告)日：2006-07-06

申请号：US10526985

申请日：2003-08-28

申请人： Naohiro Noda ,  Takuya Onodera ,  Koji Maruyama ,  Takeshi Saika ,  Yasunobu Tanaka ,  Masao Nasu ,  Nobuyasu Yamaguchi

发明人： Naohiro Noda ,  Takuya Onodera ,  Koji Maruyama ,  Takeshi Saika ,  Yasunobu Tanaka ,  Masao Nasu ,  Nobuyasu Yamaguchi

IPC分类号： C12Q1/04

CPC分类号： G01N15/0612 ,  C12Q1/04 ,  C12Q1/24 ,  G01N2015/1452

摘要： A method for detecting microorganisms or cells wherein microorganisms or cells contained in a sample are captured on the surface of the adhesive layer of a collection sheet having a substrate layer containing a focusing marker, an adhesive layer having a predetermined thickness and deposited on this substrate layer, the microorganisms or cells are stained by a staining reagent, and after being automatically focused, the light receiving optical system or the collection sheet is moved relatively by a distance equivalent to that obtained by adding the distance from the surface of the substrate layer to the position of the focusing marker to the predetermined thickness of the adhesive layer from the focusing position by this autofocusing reference point, the microorganisms or cells on the adhesive layer are automatically focused, and a light is radiated on the focused adhesive layer to measure the image and detect the microorganisms or cells.

摘要翻译： 一种用于检测微生物或细胞的方法，其中包含在样品中的微生物或细胞被捕获在具有包含聚焦标记的基底层的粘合剂层的表面上，具有预定厚度的粘合剂层并沉积在该基底层上 ，微生物或细胞被染色试剂染色，并且在被自动聚焦之后，光接收光学系统或收集纸移动相对于通过将从基底层表面延伸到 通过该自动聚焦参考点将聚焦标记的位置从聚焦位置到粘合剂层的预定厚度，粘合剂层上的微生物或细胞自动聚焦，并且将光照射在聚焦的粘合剂层上以测量图像， 检测微生物或细胞。

公开(公告)号：US06203900B1

公开(公告)日：2001-03-20

申请号：US08885848

申请日：1997-06-30

申请人： Takeshi Saika ,  Shuji Senda ,  Yoshitaka Kazuse ,  Akio Iwama ,  Tetsuji Sugii ,  Masao Nasu ,  Katsuji Tani ,  Nobuyasu Yamaguchi

发明人： Takeshi Saika ,  Shuji Senda ,  Yoshitaka Kazuse ,  Akio Iwama ,  Tetsuji Sugii ,  Masao Nasu ,  Katsuji Tani ,  Nobuyasu Yamaguchi

IPC分类号： B32B1504

CPC分类号： C12Q1/04 ,  G01N33/569 ,  G01N33/56916 ,  Y10T428/28 ,  Y10T428/283 ,  Y10T428/2891

摘要： A pressure sensitive adhesive sheet for the detection of microorganisms, which comprises a laminate of an adhesive layer mainly composed of a water-soluble polymer and a water-permeable membrane which does not allow passage of the microorganisms; a pressure sensitive adhesive sheet for the detection of microorganisms, wherein the surface of the adhesive layer has a contact angle with water of not more than 90°; and a method for detecting a microorganism, which comprises bringing the surface of an adhesive layer of a pressure sensitive adhesive sheet into contact with the surface of a test object, and then bringing the surface of the adhesive layer into contact with water, said adhesive layer or water containing a chromogenic reagent. The use of the laminate makes it possible to leave only stained microorganisms on the water-permeable membrane and to precisely observe the color developed by the microorganisms. The method of the present invention enables real-time monitoring without culture of microorganisms, and is not limited to the detection of viable cells alone. Inasmuch as a contact of the pressure sensitive adhesive sheet with a test face results in accumulation of microorganisms, the manipulation is simple. In addition, by properly determining a chromogenic reagent, a specific microorganism alone can be detected and counted, so that it can be applied to environmental investigation in the medical field, food industry field and the like.

摘要翻译： 一种用于检测微生物的压敏粘合片，其包括主要由水溶性聚合物组成的粘合剂层和不允许微生物通过的水渗透膜的层压体; 用于检测微生物的压敏粘合片，其中所述粘合剂层的表面与水的接触角不大于90°; 以及检测微生物的方法，其特征在于，使粘合剂层的粘合剂层的表面与试验体的表面接触，然后使粘合剂层的表面与水接触，所述粘合层 或含有显色试剂的水。 使用层压体可以在透水膜上仅留下染色的微生物，并精确地观察由微生物显色的颜色。 本发明的方法能够在不培养微生物的情况下进行实时监测，并不限于单独检测活细胞。 由于压敏粘合片与测试面的接触导致微生物的积累，操作简单。 此外，通过适当地测定显色试剂，可以检测和计数单独的特定微生物，从而可以应用于医疗领域，食品工业领域等的环境调查。