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    Adhesive sheet and kit for microbial testing of solid surface
    1.
    发明申请
    Adhesive sheet and kit for microbial testing of solid surface 审中-公开
    用于固体表面微生物测试的粘合片和试剂盒

    公开(公告)号：US20050208295A1

    公开(公告)日：2005-09-22

    申请号：US10526794

    申请日：2003-09-03

    申请人： Takeshi Saika ,  Koji Maruyama ,  Yasunobu Tanaka ,  Naohiro Noda ,  Takuya Onodera ,  Masao Nasu ,  Nobuyasu Yamaguchi

    发明人： Takeshi Saika ,  Koji Maruyama ,  Yasunobu Tanaka ,  Naohiro Noda ,  Takuya Onodera ,  Masao Nasu ,  Nobuyasu Yamaguchi

    IPC分类号： C12M1/26 ,  C12Q1/06 ,  B32B7/12 ,  B32B15/04

    CPC分类号： C12Q1/06 ,  Y10T428/28 ,  Y10T428/2848

    摘要： The present invention provides an adhesive sheet and a kit for microbial testing, which enable monitoring of the presence of a microorganism and/or the cell count thereof on a solid surface conveniently on a real time basis, and which accommodate to automated focusing during image analysis. The present invention relates to an adhesive sheet for microbial testing having at least a substrate and an adhesive layer, which adhesive layer is compression-bonded to, and peeled from, the surface of a test article to collect microorganisms, and the surface of the adhesive layer is then subjected to image analysis, wherein a marker for focusing the image is provided in the substrate, or in the adhesive layer, or on the surface thereof.

    摘要翻译： 本发明提供一种用于微生物测试的粘合片和用于微生物测试的试剂盒，其能够在实时基础上方便地监测微生物的存在和/或其细胞计数，并且其可适应于图像分析期间的自动聚焦 。 本发明涉及一种用于微生物测试的粘合片，其至少具有基材和粘合剂层，该粘合剂层被压制粘合并从测试物品的表面上剥离以收集微生物，并且粘合剂的表面 然后对层进行图像分析，其中用于聚焦图像的标记设置在基板中，或者在粘合剂层中或其表面上。

    Method for detecting microbe or cell
    2.
    发明申请
    Method for detecting microbe or cell 审中-公开
    检测微生物或细胞的方法

    公开(公告)号：US20060148028A1

    公开(公告)日：2006-07-06

    申请号：US10526985

    申请日：2003-08-28

    申请人： Naohiro Noda ,  Takuya Onodera ,  Koji Maruyama ,  Takeshi Saika ,  Yasunobu Tanaka ,  Masao Nasu ,  Nobuyasu Yamaguchi

    发明人： Naohiro Noda ,  Takuya Onodera ,  Koji Maruyama ,  Takeshi Saika ,  Yasunobu Tanaka ,  Masao Nasu ,  Nobuyasu Yamaguchi

    IPC分类号： C12Q1/04

    CPC分类号： G01N15/0612 ,  C12Q1/04 ,  C12Q1/24 ,  G01N2015/1452

    摘要： A method for detecting microorganisms or cells wherein microorganisms or cells contained in a sample are captured on the surface of the adhesive layer of a collection sheet having a substrate layer containing a focusing marker, an adhesive layer having a predetermined thickness and deposited on this substrate layer, the microorganisms or cells are stained by a staining reagent, and after being automatically focused, the light receiving optical system or the collection sheet is moved relatively by a distance equivalent to that obtained by adding the distance from the surface of the substrate layer to the position of the focusing marker to the predetermined thickness of the adhesive layer from the focusing position by this autofocusing reference point, the microorganisms or cells on the adhesive layer are automatically focused, and a light is radiated on the focused adhesive layer to measure the image and detect the microorganisms or cells.

    摘要翻译： 一种用于检测微生物或细胞的方法，其中包含在样品中的微生物或细胞被捕获在具有包含聚焦标记的基底层的粘合剂层的表面上，具有预定厚度的粘合剂层并沉积在该基底层上 ，微生物或细胞被染色试剂染色，并且在被自动聚焦之后，光接收光学系统或收集纸移动相对于通过将从基底层表面延伸到 通过该自动聚焦参考点将聚焦标记的位置从聚焦位置到粘合剂层的预定厚度，粘合剂层上的微生物或细胞自动聚焦，并且将光照射在聚焦的粘合剂层上以测量图像， 检测微生物或细胞。

    Method of counting microorganisms or cells
    3.
    发明申请
    Method of counting microorganisms or cells 审中-公开
    计数微生物或细胞的方法

    公开(公告)号：US20060073470A1

    公开(公告)日：2006-04-06

    申请号：US10515994

    申请日：2003-05-28

    申请人： Naohiro Noda ,  Mutsuhisa Hiraoka ,  Kazuhito Takahashi ,  Koji Maruyama ,  Takeshi Saika ,  Yasunobu Tanaka ,  Masao Nasu ,  Nobuyasu Yamaguchi

    发明人： Naohiro Noda ,  Mutsuhisa Hiraoka ,  Kazuhito Takahashi ,  Koji Maruyama ,  Takeshi Saika ,  Yasunobu Tanaka ,  Masao Nasu ,  Nobuyasu Yamaguchi

    IPC分类号： C12Q1/00 ,  C12Q1/04

    CPC分类号： G01N21/6456 ,  C12Q1/04 ,  G01N21/6428 ,  G01N2021/6439

    摘要： A method of counting microorganisms or cells in a sample by labeling the microorganisms or cells with a fluorescent labeling reagent, which comprises contacting the sample containing the microorganisms or cells with an adhesive sheet having an adhesive layer laminated on at least one surface of a substrate to capture the sample; before fluorescent-labeling the microorganisms or cells, obtaining a first fluorescent image of the sample; after fluorescent-labeling the microorganisms or the cells, obtaining a second fluorescent image of the sample, and then determining the difference in the number of luminous points between the first and second images, or determining a differential image between the first and second images and determining the number of luminous points in this differential image, or determining the number of luminous points among the luminous points in the second image located outside non-sensitive regions attached to the individual luminous points in the first image.

    摘要翻译： 一种通过用荧光标记试剂标记微生物或细胞来计数样品中的微生物或细胞的方法，该方法包括将含有微生物或细胞的样品与层压在基材的至少一个表面上的粘合剂层的粘合片接触到 捕获样品; 在对微生物或细胞进行荧光标记之前，获得样品的第一荧光图像; 在对微生物或细胞进行荧光标记之后，获得样品的第二荧光图像，然后确定第一和第二图像之间的发光点数量的差异，或者确定第一和第二图像之间的差分图像，并确定 该差分图像中的发光点的数量，或者确定位于附加到第一图像中的各个发光点的非敏感区域之外的第二图像中的发光点中的发光点的数量。

    Culture device and method for eukaryotic cell transfection
    7.
    发明授权
    Culture device and method for eukaryotic cell transfection 失效
    用于真核细胞转染的培养装置和方法

    公开(公告)号：US07125709B2

    公开(公告)日：2006-10-24

    申请号：US10775341

    申请日：2004-02-10

    申请人： Yasunobu Tanaka ,  Lei Yu ,  Shouping Ji

    发明人： Yasunobu Tanaka ,  Lei Yu ,  Shouping Ji

    IPC分类号： C12M1/00 ,  C12M1/16

    CPC分类号： C12N5/0068 ,  C12N2510/00 ,  C12N2533/18 ,  C12N2533/54

    摘要： A cell culture device for transfecting a eukaryotic cell is disclosed. The cell culture/transfection device may be a multiwell plate or slide which has been coated with a metal salt such as CaCl2. Methods of using the cell culture device to transfect mammalian cells and/or to monitor cell transfection are described. Kits are also described which include the cell transfection device, eukaryotic cells to be transformed and nucleic acid for transformation.

    摘要翻译： 公开了用于转染真核细胞的细胞培养装置。 细胞培养/转染装置可以是已经涂覆有金属盐如CaCl 2 2的多孔板或载玻片。 描述了使用细胞培养装置转染哺乳动物细胞和/或监测细胞转染的方法。 还描述了包括细胞转染装置，待转化的真核细胞和用于转化的核酸的试剂盒。

    Apparatus for making a color proof
    8.
    发明授权
    Apparatus for making a color proof 失效
    用于制作色彩的装置

    公开(公告)号：US5157436A

    公开(公告)日：1992-10-20

    申请号：US706150

    申请日：1991-05-28

    申请人： Masahide Akisada ,  Kazuya Fujimoto ,  Takatugu Kusayanagi ,  Yasunobu Tanaka

    发明人： Masahide Akisada ,  Kazuya Fujimoto ,  Takatugu Kusayanagi ,  Yasunobu Tanaka

    IPC分类号： G03B27/30

    CPC分类号： G03B27/30

    摘要： An apparatus for making a color proof. An original loading portion having a loading opening provided for loading the original is disposed side by side at an exposure portion so that a carriage may be substantially horizontally moved between the exposure position of the exposure portion and the copy exchange position of corresponding to the loading opening. When the original is exchanged and recharged, the loading opening is open by a lid member and when the carriage is positioned to the copy exchange position, the original is placed. Since the exposure portion and the like are not disposed upwardly of the original loading portion, the exposure unit and the like are not mounted with the result that the lid member can be made compact and lightweight while exchange of the original can be eased. In addition, the carriage is not withdrawn into the working space allowed for the operators.

    摘要翻译： 用于制作色彩的装置。 具有用于装载原件的装载开口的原始装载部分并排设置在曝光部分，使得滑架可以在曝光部分的曝光位置和对应于装载开口的复印更换位置之间基本上水平移动 。 当原件被更换和充电时，装载开口由盖构件打开，并且当托架被定位到复印交换位置时，​​放置原件。 由于曝光部分等没有设置在原始装载部分的上方，曝光单元等不被安装，从而可以使得盖构件可以被制造成紧凑轻便，同时可以更换原稿。 此外，运输工具不会撤回运营商允许的工作空间。

    Method of and apparatus for drying photographic light-sensitive material in photographic processing machine
    9.
    发明授权
    Method of and apparatus for drying photographic light-sensitive material in photographic processing machine 失效
    摄影处理机中照相感光材料的干燥方法和装置

    公开(公告)号：US4914835A

    公开(公告)日：1990-04-10

    申请号：US172687

    申请日：1988-03-23

    申请人： Junichi Kose ,  Yoshiyasu Matsuzaki ,  Motoharu Komori ,  Nobuyuki Seto ,  Miyuki Endo ,  Kimitaka Kameoka ,  Takashi Naoi ,  Yasunobu Tanaka ,  Tomoyuki Takiue

    发明人： Junichi Kose ,  Yoshiyasu Matsuzaki ,  Motoharu Komori ,  Nobuyuki Seto ,  Miyuki Endo ,  Kimitaka Kameoka ,  Takashi Naoi ,  Yasunobu Tanaka ,  Tomoyuki Takiue

    IPC分类号： F26B13/10 ,  F26B21/10 ,  G03D15/02

    CPC分类号： F26B13/10 ,  F26B21/10 ,  G03D15/022

    摘要： In a method of drying a photographic light-sensitive material by use of drying air, the amount of light-sensitive material which can be processed within a predetermined time at a set temperature of the drying air is compared with the totalized amount of the light-sensitive material to be actually processed within the predetermined time, and, on the basis of the result of this comparison, the set temperature of the drying air can be adjusted. An apparatus for drying photographic light-sensitive material by use of drying air is arranged so that the amount of light-sensitive material to be actually processed within a predetermined time is detected by a detecting sensor and the thus-detected amount is compared with the amount of light-sensitive material which can be dried. If the amount of light-sensitive material to be actually processed exceeds the amount of light-sensitive material which can be processed, such a display is conducted that the apparatus is not ready for insertion of further light-sensitive material. Accordingly, the light-sensitive material can consistently be dried at a proper drying temperature.

    摘要翻译： 在通过使用干燥空气干燥摄影感光材料的方法中，将可在干燥空气的设定温度下的预定时间内处理的感光材料的量与发光材料的合计量进行比较， 在预定时间内实际处理的敏感材料，并且基于该比较的结果，可以调节干燥空气的设定温度。 通过使用干燥空气来对照相感光材料进行干燥的装置，通过检测传感器检测在预定时间内实际处理的感光材料的量，并将这样检测的量与量 的可以干燥的感光材料。 如果实际处理的感光材料的量超过可以处理的感光材料的量，则进行这样的显示，使得该装置未准备好插入另外的感光材料。 因此，感光材料可以在适当的干燥温度下始终干燥。