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公开(公告)号：US08679815B2
公开(公告)日：2014-03-25
申请号：US11960259
申请日：2007-12-19
申请人： Toby M. Baldwin , Benjamin S. Bower , Nigel Dunn-Coleman , Suzanne E. Lantz , Michael J. Pepsin
发明人： Toby M. Baldwin , Benjamin S. Bower , Nigel Dunn-Coleman , Suzanne E. Lantz , Michael J. Pepsin
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别是有用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶的木霉宿主细胞。
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2.发明申请Acid-Stable Alpha Amylases having Granular Starch Hydrolyzing Activity and Enzyme Compositions 有权具有颗粒状淀粉水解活性和酶组成的酸稳定的α-淀粉酶公开(公告)号：US20080153136A1
公开(公告)日：2008-06-26
申请号：US12022365
申请日：2008-01-30
申请人： Nigel Dunn-Coleman , Susan M. Fiske , Suzanne E. Lantz , Paulien Neefe-Kruithof , Michael J. Pepsin , Jayarama K. Shetty
发明人： Nigel Dunn-Coleman , Susan M. Fiske , Suzanne E. Lantz , Paulien Neefe-Kruithof , Michael J. Pepsin , Jayarama K. Shetty
CPC分类号： C12N9/242 , C12N9/2417 , C12N9/2428 , C12N15/8245 , C12P7/06 , C12P7/10 , C12P19/02 , C12P19/04 , C12P19/14 , C12P19/22 , C12P21/06 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to an acid-stable alpha amylase (asAA) derived from a strain of Aspergillus kawachi, which has granular starch hydrolyzing (GSH) activity, the heterologous expression of the asAA having GSH activity in filamentous fungal host cells and enzyme compositions including the same which optionally include glucoamylase.
摘要翻译： 本发明涉及源自川曲霉菌株的酸稳定性α淀粉酶（asAA），其具有粒状淀粉水解（GSH）活性，在丝状真菌宿主细胞中具有GSH活性的asAA的异源表达和酶组合物，包括 其任选地包括葡糖淀粉酶。
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公开(公告)号：US07335503B2
公开(公告)日：2008-02-26
申请号：US11633309
申请日：2006-12-04
申请人： Toby M. Baldwin , Benjamin S. Bower , Nigel Dunn-Coleman , Suzanne E. Lantz , Michael J. Pepsin
发明人： Toby M. Baldwin , Benjamin S. Bower , Nigel Dunn-Coleman , Suzanne E. Lantz , Michael J. Pepsin
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别是有用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶的木霉宿主细胞。
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4.发明授权公开(公告)号：US08323932B2
公开(公告)日：2012-12-04
申请号：US13230707
申请日：2011-09-12
申请人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
发明人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
IPC分类号： C12P13/04 , C12P13/24 , C12P13/22 , C12P13/20 , C12P19/14 , C12P19/20 , C12P19/02 , C12N9/32 , C12N9/34 , C07H21/04
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。
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5.发明授权Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions 有权具有颗粒淀粉水解活性和酶组成的酸稳定的α-淀粉酶公开(公告)号：US07691617B2
公开(公告)日：2010-04-06
申请号：US12022365
申请日：2008-01-30
申请人： Nigel Dunn-Coleman , Susan M. Fiske , Suzanne E. Lantz , Paulien Neefe-Kruithof , Michael J. Pepsin , Jayarama K. Shetty
发明人： Nigel Dunn-Coleman , Susan M. Fiske , Suzanne E. Lantz , Paulien Neefe-Kruithof , Michael J. Pepsin , Jayarama K. Shetty
CPC分类号： C12N9/242 , C12N9/2417 , C12N9/2428 , C12N15/8245 , C12P7/06 , C12P7/10 , C12P19/02 , C12P19/04 , C12P19/14 , C12P19/22 , C12P21/06 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to an acid-stable alpha amylase (asAA) derived from a strain of Aspergillus kawachi, which has granular starch hydrolyzing (GSH) activity, the heterologous expression of the asAA having GSH activity in filamentous fungal host cells and enzyme compositions including the same which optionally include glucoamylase.
摘要翻译： 本发明涉及源自川曲霉菌株的酸稳定性α淀粉酶（asAA），其具有粒状淀粉水解（GSH）活性，在丝状真菌宿主细胞中具有GSH活性的asAA的异源表达和酶组合物，包括 其任选地包括葡糖淀粉酶。
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6.发明授权公开(公告)号：US07205138B2
公开(公告)日：2007-04-17
申请号：US11347886
申请日：2006-02-06
CPC分类号： C12P7/06 , C12N9/242 , C12N9/2428 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25–40° C. to obtain a fermentation broth having 5 to 20% ethanol.
摘要翻译： 本发明涉及含有具有淀粉淀粉水解活性的酸稳定性α淀粉酶（asAA）的粒状淀粉水解酶组合物。 本发明还涉及一种生产醇的一步法，该方法包括在发酵步骤中使颗粒淀粉基质与具有颗粒状淀粉水解（GSH）活性的酸稳定性α淀粉酶（asAA）和葡糖淀粉酶（GA）接触， 还在25-40℃的温度下包含产乙醇微生物以获得具有5至20％乙醇的发酵液。
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7.发明授权公开(公告)号：US08034590B2
公开(公告)日：2011-10-11
申请号：US11875279
申请日：2007-10-19
申请人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
发明人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。
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8.发明申请Expression of Granular Starch Hydrolyzing Enzymes in Trichoderma and process for producing glucose from granular starch substrates 有权颗粒状淀粉水解酶在木霉菌中的表达及由颗粒淀粉基质生产葡萄糖的方法公开(公告)号：US20090203087A1
公开(公告)日：2009-08-13
申请号：US11875279
申请日：2007-10-19
申请人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, JR. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
发明人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, JR. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。
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9.发明授权Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions 有权具有颗粒淀粉水解活性和酶组成的酸稳定的α-淀粉酶公开(公告)号：US07354752B2
公开(公告)日：2008-04-08
申请号：US11136244
申请日：2005-05-24
申请人： Nigel Dunn-Coleman , Susan M. Fiske , Suzanne E. Lantz , Paulien Neefe-Kruithof , Michael J. Pepsin , Jayarama K. Shetty
发明人： Nigel Dunn-Coleman , Susan M. Fiske , Suzanne E. Lantz , Paulien Neefe-Kruithof , Michael J. Pepsin , Jayarama K. Shetty
CPC分类号： C12N9/242 , C12N9/2417 , C12N9/2428 , C12N15/8245 , C12P7/06 , C12P7/10 , C12P19/02 , C12P19/04 , C12P19/14 , C12P19/22 , C12P21/06 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to an acid-stable alpha amylase (asAA) derived from a strain of Aspergillus kawachi, which has granular starch hydrolyzing (GSH) activity, the heterologous expression of the asAA having GSH activity in filamentous fungal host cells and enzyme compositions including the same which optionally include glucoamylase.
摘要翻译： 本发明涉及源自川曲霉菌株的酸稳定性α淀粉酶（asAA），其具有粒状淀粉水解（GSH）活性，在丝状真菌宿主细胞中具有GSH活性的asAA的异源表达和酶组合物，包括 其任选地包括葡糖淀粉酶。
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10.发明授权公开(公告)号：US07037704B2
公开(公告)日：2006-05-02
申请号：US10999886
申请日：2004-11-30
申请人： Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty
发明人： Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty
CPC分类号： C12P7/06 , C12N9/242 , C12N9/2428 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25–40° C. to obtain a fermentation broth having 5 to 20% ethanol.
摘要翻译： 本发明涉及含有具有淀粉淀粉水解活性的酸稳定性α淀粉酶（asAA）的粒状淀粉水解酶组合物。 本发明还涉及一种生产醇的一步法，该方法包括在发酵步骤中使颗粒淀粉基质与具有颗粒状淀粉水解（GSH）活性的酸稳定性α淀粉酶（asAA）和葡糖淀粉酶（GA）接触， 还在25-40℃的温度下包含产乙醇微生物以获得具有5至20％乙醇的发酵液。
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