GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS
    1.
    发明申请
    GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS 有权
    生产油脂的高聚羟基树脂的生成

    公开(公告)号:US20120180162A1

    公开(公告)日:2012-07-12

    申请号:US13395702

    申请日:2010-09-15

    CPC分类号: C12N15/8214 C12N15/8257

    摘要: Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates (“PHA”) are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate (“PHB”). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

    摘要翻译: 提供了用于合成生物聚合物的转基因植物,植物材料,植物细胞和遗传构建体,例如聚羟基链烷酸酯(“PHA”)。 在一个实施方案中,转基因植物合成聚羟基丁酸酯(“PHB”)。 在一个实施方案中,转基因植物编码编码用于产生PHA的酶的一种或多种基因的siRNA。 在更优选的实施方案中,siRNA表达受诱导型调节元件的控制。 在另一个实施方案中,转基因植物含有编码将降解聚合物的表达酶的转基因。 在优选的实施方案中,这些酶的表达受发芽特异性,诱导型或最小启动子的控制。 在另一个实施方案中,转基因植物含有编码增加聚合物合成碳流的酶的转基因。 在优选的实施方案中,这些转基因编码增加卡尔文循环中碳流的酶。

    Generation of high polyhydroxybutyrate producing oilseeds
    2.
    发明授权
    Generation of high polyhydroxybutyrate producing oilseeds 有权
    生产高聚羟基丁酸酯油籽油

    公开(公告)号:US09181559B2

    公开(公告)日:2015-11-10

    申请号:US13395702

    申请日:2010-09-15

    CPC分类号: C12N15/8214 C12N15/8257

    摘要: Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates (“PHA”) are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate (“PHB”). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

    摘要翻译: 提供了用于合成生物聚合物的转基因植物,植物材料,植物细胞和遗传构建体,例如聚羟基链烷酸酯(“PHA”)。 在一个实施方案中,转基因植物合成聚羟基丁酸酯(“PHB”)。 在一个实施方案中,转基因植物编码编码用于产生PHA的酶的一种或多种基因的siRNA。 在更优选的实施方案中,siRNA表达受诱导型调节元件的控制。 在另一个实施方案中,转基因植物含有编码将降解聚合物的表达酶的转基因。 在优选的实施方案中,这些酶的表达受发芽特异性,诱导型或最小启动子的控制。 在另一个实施方案中,转基因植物含有编码增加聚合物合成碳流的酶的转基因。 在优选的实施方案中,这些转基因编码增加卡尔文循环中碳流的酶。

    METHOD OF POSITIVE PLANT SELECTION USING SORBITOL DEHYDROGENASE
    7.
    发明申请
    METHOD OF POSITIVE PLANT SELECTION USING SORBITOL DEHYDROGENASE 审中-公开
    使用SORBITOL DEHYDROGENASE的积极植物选择方法

    公开(公告)号:US20110321190A1

    公开(公告)日:2011-12-29

    申请号:US13223575

    申请日:2011-09-01

    CPC分类号: C12N15/821 C12N9/0006

    摘要: Transgenic plants and methods of culturing them using sorbitol as a sole carbon source are provided. One embodiment provides a method and system for positively selecting transgenic plants carrying and expressing a gene of interest. The transgenic plants are engineered to express sorbitol dehydrogenase in an amount effective to allow the transgenic plant to grow using sorbitol as the sole carbon source. In a preferred embodiment, the plant to be transformed does not have endogenous sorbitol dehydrogenase activity. Representative plants that can be transformed, include but are not limited to members of the Brassica family, industrial oilseeds, Arabidopsis thaliana, algae, soybean, cottonseed, sunflower, palm, coconut, rice, safflower, peanut, mustards, silage corn, alfalfa, switchgrass, miscanthus, sorghum, tobacco, sugarcane and flax.

    摘要翻译: 提供了转基因植物和使用山梨醇作为唯一碳源培养它们的方法。 一个实施方案提供了用于正选择携带并表达感兴趣的基因的转基因植物的方法和系统。 转基因植物被工程化以表达山梨糖醇脱氢酶,其数量有效地允许转基因植物使用山梨糖醇作为唯一碳源来生长。 在优选的实施方案中,待转化的植物不具有内源山梨醇脱氢酶活性。 可以转化的代表性植物包括但不限于芸苔属家族,工业油籽,拟南芥,藻类,大豆,棉籽,向日葵,棕榈,椰子,大米,红花,花生,芥菜,青贮玉米,苜蓿, 柳枝稷,芒草,高粱,烟草,甘蔗和亚麻。

    PRODUCTION OF POLYHYDROXYBUTYRATE IN SWITCHGRASS
    8.
    发明申请
    PRODUCTION OF POLYHYDROXYBUTYRATE IN SWITCHGRASS 失效
    在开关中生产聚羟基丁酸酯

    公开(公告)号:US20090271889A1

    公开(公告)日:2009-10-29

    申请号:US12431428

    申请日:2009-04-28

    IPC分类号: A01H5/00 C12N15/82 C08L97/02

    摘要: Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.

    摘要翻译: 提供用于合成聚羟基链烷酸酯,优选聚(3-羟基丁酸酯)(也称为PHB)的转基因植物,植物材料和植物细胞。 可以进行遗传工程以生产PHB的优选植物包括通常不产生储存产物如油和碳水化合物的植物,以及具有C4 NAD-苹果酸光合途径的植物。 这样的植物还有利地生产可以转化为生物燃料的木质纤维素生物质。 可以遗传工程化生产PHB并生产木质纤维素生物质的示范性植物是柳枝稷(Panicum virgatum L.)。优选的柳枝稷品种是Alamo。 柳枝稷的其他合适品种包括但不限于Blackwell,Kanlow,Nebraska 28,Pathfinder,Cave-in-Rock,Shelter和Trailblazer。

    Modification of fatty acid metabolism in plants
    9.
    发明授权
    Modification of fatty acid metabolism in plants 失效
    植物脂肪酸代谢的修饰

    公开(公告)号:US06586658B1

    公开(公告)日:2003-07-01

    申请号:US09389395

    申请日:1999-09-03

    IPC分类号: A01H100

    摘要: Methods and systems to modify fatty acid biosynthesis and oxidation in plants to make new polymers are provided. Two enzymes are essential: a hydratase such as D-specific enoyl-CoA hydratase, for example, the hydratase obtained from Aeromonas caviae, and a &bgr;-oxidation enzyme system. Some plants have a &bgr;-oxidation enzyme system which is sufficient to modify polymer synthesis when the plants are engineered to express the hydratase. Examples demonstrate production of polymer by expression of these enzymes in transgenic plants. Examples also demonstrate that modifications in fatty acid biosynthesis can be used to alter plant phenotypes, decreasing or eliminating seed production and increasing green plant biomass, as well as producing polyhydroxyalkanoates.

    摘要翻译: 提供了修饰植物脂肪酸生物合成和氧化以制备新聚合物的方法和系统。 两种酶是必需的:水合酶如D-特异性烯酰辅酶A水合酶,例如从气单胞菌(Aeromonas caviae)获得的水合酶和β-氧化酶系统。 一些植物具有β-氧化酶系统,当植物被工程化以表达水合酶时,其足以改变聚合物合成。 实施例证明通过在转基因植物中表达这些酶来生产聚合物。 实例还表明,脂肪酸生物合成中的修饰可用于改变植物表型,减少或消除种子生产和增加绿色植物生物量,以及生产聚羟基链烷酸酯。

    Production of polyhydroxybutyrate in switchgrass
    10.
    发明授权
    Production of polyhydroxybutyrate in switchgrass 失效
    柳枝稷中聚羟基丁酸酯的生产

    公开(公告)号:US08487159B2

    公开(公告)日:2013-07-16

    申请号:US12431428

    申请日:2009-04-28

    摘要: Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.

    摘要翻译: 提供用于合成聚羟基链烷酸酯,优选聚(3-羟基丁酸酯)(也称为PHB)的转基因植物,植物材料和植物细胞。 可以进行遗传工程以生产PHB的优选植物包括通常不产生储存产物如油和碳水化合物的植物,以及具有C4 NAD-苹果酸酶光合途径的植物。 这样的植物还有利地生产可以转化为生物燃料的木质纤维素生物质。 可以遗传工程化生产PHB并生产木质纤维素生物质的示范性植物是柳枝稷(Panicum virgatum L.)。优选的柳枝稷品种是Alamo。 柳枝稷的其他合适品种包括但不限于Blackwell,Kanlow,Nebraska 28,Pathfinder,Cave-in-Rock,Shelter和Trailblazer。