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公开(公告)号:US20110224420A1
公开(公告)日:2011-09-15
申请号:US13110473
申请日:2011-05-18
CPC分类号: G01N33/54326 , G01N33/5308 , G01N33/5695 , G01N2333/916
摘要: The present invention provides a solid support for performing steps of isolation of cell or extraction and purification of nucleic acid, safely, easily, efficiently, and with high yield in the genetic test for investigating the presence of pathogenic bacterial infection. A solid support for binding with cell as an embodiment of the above-described solid support, comprises a polypeptide having capability of binding with my colic acid-containing glycolipid which is immobilized on the surface of a carrier. In addition, a solid support for binding with nucleic acid as another embodiment of the above-described solid support, comprises a polypeptide having capability of binding with nucleic acid which is immobilized on the surface of a carrier.
摘要翻译: 本发明提供了用于在用于调查病原性细菌感染的存在的遗传测试中进行细胞分离或核酸提取和纯化的步骤的安全,容易,有效和高产率的固体支持。 作为上述固体支持物的实施方案,与细胞结合的固体支持物包含能够与我的含有酸性链烷酸的糖脂结合的多肽,其被固定在载体的表面上。 此外,作为上述固体支持物的另一个实施方案与核酸结合的固体支持物包含具有与固定在载体表面上的核酸结合的能力的多肽。
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公开(公告)号:US20090130680A1
公开(公告)日:2009-05-21
申请号:US12212839
申请日:2008-09-18
CPC分类号: G01N33/54326 , G01N33/5308 , G01N33/5695 , G01N2333/916
摘要: The present invention provides a solid support for performing steps of isolation of cell or extraction and purification of nucleic acid, safely, easily, efficiently, and with high yield in the genetic test for investigating the presence of pathogenic bacterial infection. A solid support for binding with cell as an embodiment of the above-described solid support, comprises a polypeptide having capability of binding with mycolic acid-containing glycolipid which is immobilized on the surface of a carrier. In addition, a solid support for binding with nucleic acid as another embodiment of the above-described solid support, comprises a polypeptide having capability of binding with nucleic acid which is immobilized on the surface of a carrier.
摘要翻译: 本发明提供了用于在用于调查病原性细菌感染的存在的遗传测试中进行细胞分离或核酸提取和纯化的步骤的安全,容易,有效和高产率的固体支持。 作为上述固体支持物的实施方案,与细胞结合的固体支持物包含具有与含有霉菌酸的糖脂结合的能力的多肽,其固定在载体的表面上。 此外,作为上述固体支持物的另一个实施方案与核酸结合的固体支持物包含具有与固定在载体表面上的核酸结合的能力的多肽。
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公开(公告)号:US20080171337A1
公开(公告)日:2008-07-17
申请号:US12013033
申请日:2008-01-11
申请人: Koji MIYAZAKI , Akihisa Nakajima , Kanako Usui , Fumitsugu HINO , HIroyuki MUKAI , Ikunoshin KATO
发明人: Koji MIYAZAKI , Akihisa Nakajima , Kanako Usui , Fumitsugu HINO , HIroyuki MUKAI , Ikunoshin KATO
CPC分类号: C12N15/1013
摘要: A method for isolating a nucleic acid from a cell-containing sample by using a magnet with a removable cover attached thereto and a plurality of containers arranged on a table, the plurality of containers having (1) a first container for mixing a cell-containing sample and magnetic beads to which cells can be adhered, (2) a cleaning tank for cleaning the magnetic beads with cells adhering thereto, and (3) a second container for heating the cleaned magnetic beads, the method includes: mixing a liquid including cell-containing sample and magnetic beads in the cell-containing sample in the first container so as to adhere cells in the cell-containing sample to magnetic beads; taking out the magnetic beads from the first container and cleaning the magnetic beads; and suspending the magnetic beads in a re-suspension buffer in the second container and heating the second container at 80 to 120° C. for 20 to 30°.
摘要翻译: 一种通过使用附着有可移除盖的磁体和布置在桌子上的多个容器从含细胞样品中分离核酸的方法,所述多个容器具有(1)第一容器,用于混合含细胞的 可以粘附细胞的样品和磁珠,(2)用于清洁附着有细胞的磁珠的清洗槽,和(3)用于加热清洁的磁珠的第二容器,该方法包括: 在第一容器中的含细胞样品中的样品和磁珠,以将细胞含有样品中的细胞粘附到磁珠上; 从第一容器中取出磁珠并清洁磁珠; 并将磁珠悬浮在第二容器中的再悬浮缓冲液中,并将第二容器在80至120℃下加热20至30℃。
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公开(公告)号:US20080253747A1
公开(公告)日:2008-10-16
申请号:US11870727
申请日:2007-10-11
申请人: Koji MIYAZAKI
发明人: Koji MIYAZAKI
IPC分类号: H04N7/26
CPC分类号: H04N21/4334 , H04N5/64 , H04N5/76 , H04N5/765 , H04N5/781 , H04N5/85 , H04N5/907 , H04N21/4135 , H04N21/47
摘要: A recording apparatus comprises: a removable recording medium; a processor; and a receiving unit that receives an operation command for demounting the removable recording medium, wherein, when the receiving unit receives the operation command, the processor demounts the removable recording medium from a file system; and wherein the processor automatically mounts the recording medium on the file system under a predetermined condition in which a command of mounting the recording medium is not given.
摘要翻译: 记录装置包括:可移动记录介质; 处理器 以及接收单元,其接收用于拆卸所述可移动记录介质的操作命令,其中,当所述接收单元接收到所述操作命令时,所述处理器从文件系统拆卸所述可移动记录介质; 并且其中处理器在没有给出安装记录介质的命令的预定条件下自动将记录介质安装在文件系统上。
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