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公开(公告)号：US5445948A
公开(公告)日：1995-08-29
申请号：US124075
申请日：1993-09-21
IPC分类号： C12N15/09 , C12M1/12 , C12N1/00 , C12N1/10 , C12N1/16 , C12N1/20 , C12N1/21 , C12N1/38 , C12N5/00 , C12N5/10 , C12N9/38 , C12N15/00 , C12N15/70 , C12P1/00 , C12R1/19 , C12P21/00 , C12N15/03 , C12N15/71
CPC分类号： C12N1/38 , C12N15/70 , C12N9/2471 , C12P1/00 , C12Y302/01023
摘要： This invention relates to an efficient process for culturing recombinant cells which comprises a step of removing a cell proliferation inhibiting substance and/or a substance which inhibits induction of the desired product and a step of inducing production of the desired product.
摘要翻译： 本发明涉及一种培养重组细胞的有效方法，其包括除去细胞增殖抑制物质和/或抑制所需产物诱导的物质的步骤和诱导产生所需产物的步骤。
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2.发明授权
公开(公告)号：US5139938A
公开(公告)日：1992-08-18
申请号：US157719
申请日：1988-02-19
CPC分类号： C12M41/32 , C12M41/48 , C12P1/00 , C12P21/02 , Y10S435/849
摘要： Cultivation of microorganisms or animal cells or plant cells is carried out to produce high density cultivation, high cell yield and high production of desired metabolite products by monitoring acetate concentration in culture broth and regulating assimilation of acetate in the culture broth by the microorganisms or animal cells or plant cells to control the acetate concentration to a set value or less. Preferably, an acetic acid-producing bacterium that is inhibited by acetic acid and is capable of assimilating acetic acid is cultured to produce biologically active substances such as enzymes. The bacterium may be a recombinant Escherichia coli and an inducer which acts on a promoter in an expression vector is added to produce the desired metabolite product. The inducer is 3-.beta.- indolylacrylic acid when the promoter is trp-promoter or isopropyl-.beta.-D-thiogalactoside when the promoter is lac-promoter or tacpromoter.
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