-
公开(公告)号：US20070178565A1
公开(公告)日：2007-08-02
申请号：US11730549
申请日：2007-04-02
CPC分类号： C12P17/10 , C12N9/0006 , C12P7/02 , C12P7/22
摘要： The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH for activity: 5.5 to 6.0; (3) optimum temperature for activity: 50° C. to 55° C.; (4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译： 本发明提供了有效形成（R）-N-苄基-3-吡咯烷醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADH或NADPH作为辅酶作用于N-苄基-3-吡咯烷酮，形成（R） - N-苄基-3-吡咯烷醇; （2）活性最佳pH：5.5〜6.0; （3）活性最佳温度：50〜55℃。 （4）通过凝胶过滤分析确定的分子量：约55,000，通过SDS聚丙烯酰胺凝胶电泳分析确定约28,000。 本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。
-
公开(公告)号：US07220564B2
公开(公告)日：2007-05-22
申请号：US10528000
申请日：2003-09-19
CPC分类号： C12P17/10 , C12N9/0006 , C12P7/02 , C12P7/22
摘要： The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same.The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH for activity: 5.5 to 6.0; (3) optimum temperature for activity: 50° C. to 55° C.; (4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译： 本发明提供了有效形成（R）-N-苄基-3-吡咯烷醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADH或NADPH作为辅酶作用于N-苄基-3-吡咯烷酮，形成（R） - N-苄基-3-吡咯烷醇; （2）活性最佳pH：5.5〜6.0; （3）活性最佳温度：50〜55℃。 （4）通过凝胶过滤分析确定的分子量：约55,000，通过SDS聚丙烯酰胺凝胶电泳分析确定约28,000。 本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。
-
公开(公告)号：US07531329B2
公开(公告)日：2009-05-12
申请号：US11730549
申请日：2007-04-02
CPC分类号： C12P17/10 , C12N9/0006 , C12P7/02 , C12P7/22
摘要： The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the physical and chemical properties (1) to (4): (1) activity acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH of 5.5 to 6.0 for activity ; (3) optimum temperature of 50° C. to 55° C. for activity ; (4) molecular weight of about 55,000 as determined by gel filtration analysis, and about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence of SEQ ID NO:1, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译： 本发明提供了有效形成（R）-N-苄基-3-吡咯烷醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有物理和化学性质（1）至（4）的多肽：（1）以NADH或NADPH作为辅酶作用于N-苄基-3-吡咯烷酮的活性以形成（R）-N- 苄基-3-吡咯烷醇; （2）活性最佳pH为5.5〜6.0; （3）活性最佳温度为50〜55℃; （4）通过凝胶过滤分析测定的约55,000的分子量，通过SDS聚丙烯酰胺凝胶电泳分析测定的约28,000。 本发明还涉及包含SEQ ID NO：1的氨基酸序列，编码所述多肽的多核苷酸和以高水平产生所述多肽的转化体的多肽。
-
公开(公告)号：US20060035357A1
公开(公告)日：2006-02-16
申请号：US10528000
申请日：2003-09-19
IPC分类号： C12N9/02
CPC分类号： C12P17/10 , C12N9/0006 , C12P7/02 , C12P7/22
摘要： The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH for activity: 5.5 to 6.0; (3) optimum temperature for activity: 50° C. to 55° C.; (4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译： 本发明提供了有效形成（R）-N-苄基-3-吡咯烷醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADH或NADPH作为辅酶作用于N-苄基-3-吡咯烷酮，形成（R） - N-苄基-3-吡咯烷醇; （2）活性最佳pH：5.5〜6.0; （3）活性最佳温度：50〜55℃。 （4）通过凝胶过滤分析确定的分子量：约55,000，通过SDS聚丙烯酰胺凝胶电泳分析确定约28,000。 本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。
-
公开(公告)号：US08129163B2
公开(公告)日：2012-03-06
申请号：US12516388
申请日：2007-11-27
CPC分类号： C12P7/22 , C12N9/0006 , C12P7/04 , Y02E50/17
摘要： An object of the present invention is to provide a novel alcohol dehydrogenase, a gene for the alcohol dehydrogenase, a vector including the gene, a transformant transformed with the vector, and a method for producing an optically active alcohol by utilizing them. A feature of the present invention directs to a novel polypeptide isolated from Candida maltosa, a DNA coding for the polypeptide, and a transformant producing the polypeptide. Another feature of the present invention directs to a method for producing an optically-active alcohol by reducing a carbonyl compound with the polypeptide or the transformant.
摘要翻译： 本发明的目的是提供一种新的醇脱氢酶，用于醇脱氢酶的基因，包含该基因的载体，用载体转化的转化体，以及通过利用它们制备光学活性醇的方法。 本发明的特征指导从恶臭假丝酵母分离的新型多肽，编码多肽的DNA和产生多肽的转化体。 本发明的另一个特征指出通过用多肽或转化体还原羰基化合物来制备光学活性醇的方法。
-
6.发明授权Method for production of erythro-or threo-2-amino-3-hydroxypropionic acid ester, novel carbonyl reductase, gene for the reductase, vector, transformant, and method for production of optically active alcohol using those 有权用于生产红血球或苏氨酸-2-氨基-3-羟基丙酸酯的方法，新的羰基还原酶，还原酶的基因，载体，转化体和使用这些的生产光学活性醇的方法公开(公告)号：US08304216B2
公开(公告)日：2012-11-06
申请号：US12225683
申请日：2007-03-29
申请人： Tozo Nishiyama , Yoshihiko Yasohara
发明人： Tozo Nishiyama , Yoshihiko Yasohara
CPC分类号： C12P13/04 , C12N9/0006
摘要： The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus Brevundimonas; a DNA coding for the polypeptide; and a transformant producing the polypeptide. The invention also relates to a method of producing an optically active alcohol by reducing a carbonyl compound with the help of the polypeptide or the transformant.
摘要翻译： 本发明的目的是提供一种制备赤式或苏氨酸-2-氨基-3-羟基丙酸酯的方法等。 本发明涉及：通过使微生物的细胞在其上作用而不对称地还原N-2-氨基-3-氧代丙酸酯的方法; 具有不对称还原羰基化合物的活性的多肽，得到从Brevundimonas属的微生物分离的光学活性醇; 编码该多肽的DNA; 和产生多肽的转化体。 本发明还涉及通过在多肽或转化体的帮助下还原羰基化合物来制备光学活性醇的方法。
-
7.发明申请NOVEL ALCOHOL DEHYDROGENASE, GENE FOR THE ALCOHOL DEHYDROGENASE, VECTOR, TRANSFORMANT, AND METHOD FOR PRODUCTION OF OPTICALLY ACTIVE ALCOHOL BY USING THEM 有权新型酒精脱氢酶，酒精脱氢酶基因，载体，转化体和使用它们生产光学活性醇的方法公开(公告)号：US20100035317A1
公开(公告)日：2010-02-11
申请号：US12516388
申请日：2007-11-27
CPC分类号： C12P7/22 , C12N9/0006 , C12P7/04 , Y02E50/17
摘要： An object of the present invention is to provide a novel alcohol dehydrogenase, a gene for the alcohol dehydrogenase, a vector including the gene, a transformant transformed with the vector, and a method for producing an optically active alcohol by utilizing them. A feature of the present invention directs to a novel polypeptide isolated from Candida maltosa, a DNA coding for the polypeptide, and a transformant producing the polypeptide. Another feature of the present invention directs to a method for producing an optically-active alcohol by reducing a carbonyl compound with the polypeptide or the transformant.
摘要翻译： 本发明的目的是提供一种新的醇脱氢酶，用于醇脱氢酶的基因，包含该基因的载体，用载体转化的转化体，以及通过利用它们制备光学活性醇的方法。 本发明的特征指导从恶臭假丝酵母分离的新型多肽，编码多肽的DNA和产生多肽的转化体。 本发明的另一个特征指出通过用多肽或转化体还原羰基化合物来制备光学活性醇的方法。
-
8.发明申请Method for Production of Erythro-or threo-2-Amino-3-Hydroxypropionic Acid Ester, Novel Carbonyl Reductase, Gene for the Reductase, Vector, Transformant, and Method for Production of Optically Active Alcohol Using Those 有权用于制备红细胞或苏氨酸-2-氨基-3-羟基丙酸酯的方法，新型羰基还原酶，还原酶的基因，载体，转化体和使用这些的生产光活性醇的方法公开(公告)号：US20090186391A1
公开(公告)日：2009-07-23
申请号：US12225683
申请日：2007-03-29
申请人： Tozo Nishiyama , Yoshihiko Yasohara
发明人： Tozo Nishiyama , Yoshihiko Yasohara
CPC分类号： C12P13/04 , C12N9/0006
摘要： The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus Brevundimonas; a DNA coding for the polypeptide; and a transformant producing the polypeptide. The invention also relates to a method of producing an optically active alcohol by reducing a carbonyl compound with the help of the polypeptide or the transformant.
摘要翻译： 本发明的目的是提供一种制备赤式或苏氨酸-2-氨基-3-羟基丙酸酯的方法等。 本发明涉及：通过使微生物的细胞在其上作用而不对称地还原N-2-氨基-3-氧代丙酸酯的方法; 具有不对称还原羰基化合物的活性的多肽，得到从Brevundimonas属的微生物分离的光学活性醇; 编码该多肽的DNA; 和产生多肽的转化体。 本发明还涉及通过在多肽或转化体的帮助下还原羰基化合物来制备光学活性醇的方法。
-
公开(公告)号：US08008461B2
公开(公告)日：2011-08-30
申请号：US11665065
申请日：2005-10-20
IPC分类号： C07H21/04 , C12N9/02 , C12P21/02 , C12P13/00 , C07C255/01
CPC分类号： C12N9/0006 , C12P7/04 , C12P7/62 , C12P13/002 , C12P41/002
摘要： The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.
摘要翻译： 本发明提供一种有效制备包含（R）-3-羟基-3-苯基丙腈的光学活性醇的方法。 本发明的特征之一是具有不对称还原从属于念珠菌属的微生物分离的3-氧代-3-苯基丙腈的活性的多肽，以产生（R）-3-羟基-3-苯基丙腈，编码 多肽和产生多肽的转化体。 本发明的另一个特征是通过使用多肽或转化体还原羰基化合物如3-氧代-3-苯基丙腈来制备光学活性醇如（R）-3-羟基-3-苯基丙腈的方法 。
-
公开(公告)号：US07332312B2
公开(公告)日：2008-02-19
申请号：US10512908
申请日：2003-04-30
CPC分类号： C12N9/0006 , C12Y101/01184
摘要： The present invention provides a novel polypeptide forming (R)-2-chloro-1-(3′-chlorophenyl)ethanol, a polynucleotide coding for said polypeptide, and use of the same.The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on 2-chloro-1-(3′-chlorophenyl)ethanone with NADPH or NADH as a coenzyme, to form (R)-2-chloro-1-(31-chlorophenyl)ethanol; (2) optimum pH for activity: 5.0 to 6.0; (3) optimum temperature for activity: 40° C. to 50° C.; (4) molecular weight: about 40,000 as determined by gel filtration analysis, about 30,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
摘要翻译： 本发明提供形成（R）-2-氯-1-（3'-氯苯基）乙醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADPH或NADH作为辅酶作用于2-氯-1-（3'-氯苯基）乙酮， 形成（R）-2-氯-1-（31-氯苯基）乙醇; （2）活性最佳pH：5.0〜6.0; （3）最适温度为40℃〜50℃。 （4）分子量：约40,000，通过凝胶过滤分析测定，约30,000，通过SDS聚丙烯酰胺凝胶电泳分析测定。 本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。
-
-
-
-
-
-
-
-
-
搜索结果
60 条记录 (耗时 0.023 秒)
