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    Gene suitable for alcohol dehydrogenase, vector and transformant
    1.
    发明授权
    Gene suitable for alcohol dehydrogenase, vector and transformant 有权
    基因适用于醇脱氢酶，载体和转化体

    公开(公告)号：US08129163B2

    公开(公告)日：2012-03-06

    申请号：US12516388

    申请日：2007-11-27

    申请人： Shigeru Kawano ,  Takeru Ishige ,  Keita Iguchi ,  Tozo Nishiyama ,  Yoshihiko Yasohara

    发明人： Shigeru Kawano ,  Takeru Ishige ,  Keita Iguchi ,  Tozo Nishiyama ,  Yoshihiko Yasohara

    IPC分类号： C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P7/22 ,  C12N9/0006 ,  C12P7/04 ,  Y02E50/17

    摘要： An object of the present invention is to provide a novel alcohol dehydrogenase, a gene for the alcohol dehydrogenase, a vector including the gene, a transformant transformed with the vector, and a method for producing an optically active alcohol by utilizing them. A feature of the present invention directs to a novel polypeptide isolated from Candida maltosa, a DNA coding for the polypeptide, and a transformant producing the polypeptide. Another feature of the present invention directs to a method for producing an optically-active alcohol by reducing a carbonyl compound with the polypeptide or the transformant.

    摘要翻译： 本发明的目的是提供一种新的醇脱氢酶，用于醇脱氢酶的基因，包含该基因的载体，用载体转化的转化体，以及通过利用它们制备光学活性醇的方法。 本发明的特征指导从恶臭假丝酵母分离的新型多肽，编码多肽的DNA和产生多肽的转化体。 本发明的另一个特征指出通过用多肽或转化体还原羰基化合物来制备光学活性醇的方法。

    Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand
    4.
    发明授权
    Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand 有权
    对免疫球蛋白具有亲和力的蛋白质和免疫球蛋白结合亲和配体

    公开(公告)号：US09403883B2

    公开(公告)日：2016-08-02

    申请号：US13258655

    申请日：2010-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C07K14/31 ,  C07K14/47 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38

    CPC分类号： C07K14/47 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3244 ,  B01J20/3274 ,  C07K14/31

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.

    摘要翻译： 本发明的一个目的是在酸存在下产生具有比常规工程改造的蛋白A配体更好的抗体解离性能的新型工程改造的蛋白A配体，本发明的另一个目的是产生一种新的工程改造的蛋白A配体，其具有 耐碱性较高。 本发明提供一种对免疫球蛋白具有亲和性的蛋白质，其包括衍生自蛋白A​​的E，D，A，B和C结构域中的任一个的氨基酸序列，其中氨基酸序列中至少一个Gly残基为 被除Ala以外的氨基酸所取代，并且该蛋白质对包含氨基酸序列的蛋白质的免疫球蛋白Fab区域的亲和力低于其中Gly残基被Ala置换的蛋白质，本发明还提供 对于免疫球蛋白具有亲和力的蛋白质，与相应的结构域相比，其在碱性条件下具有改善的化学稳定性。

    PROTEIN CAPABLE OF BINDING SPECIFICALLY TO IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND
    5.
    发明申请
    PROTEIN CAPABLE OF BINDING SPECIFICALLY TO IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND 审中-公开
    蛋白质能够特异性结合免疫球蛋白和免疫调节蛋白结合亲和力配体

    公开(公告)号：US20130096276A1

    公开(公告)日：2013-04-18

    申请号：US13636584

    申请日：2011-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C07K14/195

    CPC分类号： C07K14/195 ,  C07K1/22 ,  C07K14/31 ,  C07K16/065 ,  C07K16/32 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/92

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the acidic condition compared with known engineered Protein A ligands. The present invention provides a protein having an affinity for an immunoglobulin, including an amino acid sequence obtained by introducing, into an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, at least one amino acid substitution at any one or more of amino acid residues corresponding to positions 31 to 37 of the A, B and C domains (positions 29 to 35 of the E domain, positions 34 to 40 of the D domain), which are conserved in all the domains, the protein having a lower affinity for an Fab region of an immunoglobulin than a protein having the amino acid sequence before introduction of the substitution.

    摘要翻译： 本发明的目的是与已知的工程改造的蛋白A配体相比，在酸性条件下产生具有更好抗体解离性能的新型工程蛋白A配体。 本发明提供对免疫球蛋白具有亲和性的蛋白质，包括通过将衍生自蛋白A​​的E，D，A，B和C结构域的任何氨基酸序列导入的氨基酸序列，至少一个氨基酸 在对应于A，B和C结构域（E结构域的29至35位，D结构域的34至40位）的31至37位的氨基酸残基的任何一个或多个取代，其在所有 结构域，对于免疫球蛋白Fab区域的亲和力低于在引入取代之前具有氨基酸序列的蛋白质的蛋白质。

    PROTEIN HAVING AFFINITY FOR IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND
    6.
    发明申请
    PROTEIN HAVING AFFINITY FOR IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND 有权
    具有免疫球蛋白的免疫原性蛋白质和免疫球蛋白结合亲和力配体

    公开(公告)号：US20120208234A1

    公开(公告)日：2012-08-16

    申请号：US13258655

    申请日：2010-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C12P21/02 ,  C07K19/00 ,  C12N15/31 ,  C12N15/62 ,  C12N1/21 ,  C07K16/12 ,  C07K1/14 ,  C07K14/31 ,  C12N15/74

    CPC分类号： C07K14/47 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3244 ,  B01J20/3274 ,  C07K14/31

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.

    摘要翻译： 本发明的一个目的是在酸存在下产生具有比常规工程改造的蛋白A配体更好的抗体解离性能的新型工程改造的蛋白A配体，本发明的另一个目的是产生一种新的工程改造的蛋白A配体，其具有 耐碱性较高。 本发明提供一种对免疫球蛋白具有亲和性的蛋白质，其包括衍生自蛋白A​​的E，D，A，B和C结构域中的任一个的氨基酸序列，其中氨基酸序列中至少一个Gly残基为 被除Ala以外的氨基酸所取代，并且该蛋白质对包含氨基酸序列的蛋白质的免疫球蛋白Fab区域的亲和力低于其中Gly残基被Ala置换的蛋白质，本发明还提供 对于免疫球蛋白具有亲和力的蛋白质，与相应的结构域相比，其在碱性条件下具有改善的化学稳定性。