公开(公告)号：US10968428B2

公开(公告)日：2021-04-06

申请号：US15508749

申请日：2017-03-03

Applicant: OSAKA UNIVERSITY

Inventor： Kohji Nishida ,  Motokazu Tsujikawa ,  Susumu Hara ,  Satoshi Kawasaki ,  Masahito Yoshihara ,  Masayoshi Itoh ,  Hideya Kawaji ,  Hiroko Ohmiya

IPC: C12N5/079 ,  C12Q1/68 ,  C12N15/09 ,  C12N5/10 ,  A61K35/30 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/686 ,  G01N33/569 ,  A61L27/00

Abstract: A molecular marker expressed specifically in corneal endothelial cells, and a method for producing one or more corneal endothelial cells using the marker and a method for evaluating one or more corneal endothelial cells using the marker are provided. At least one molecule selected from the group consisting of ZP4, MRGPRX3, GRIP1, GLP1R, HTR1D, and CLRN1 is used as a marker specific to corneal endothelial cells.

公开(公告)号：US11839697B2

公开(公告)日：2023-12-12

申请号：US16621528

申请日：2018-06-19

Applicant: Osaka University

Inventor： Kohji Nishida ,  Motokazu Tsujikawa ,  Susumu Hara ,  Satoshi Kawasaki ,  Masahito Yoshihara ,  Masayoshi Itoh ,  Hideya Kawaji

IPC: A61L27/38 ,  C12Q1/06 ,  C12Q1/6869 ,  A61K35/545 ,  C12N5/079 ,  A61K35/30 ,  C12Q1/6883 ,  A61K35/28

CPC classification number: A61L27/3808 ,  A61K35/30 ,  A61K35/545 ,  A61L27/38 ,  C12N5/0621 ,  C12Q1/06 ,  C12Q1/6869 ,  A61K35/28 ,  C12Q1/6883 ,  C12Q2600/158

Abstract: Provided is a means for identifying cells suitable for transplantation into a cornea, or a means for producing a cell population suitable for transplantation into a cornea using the same. A method for producing a cell population suitable for transplantation into a cornea, the method comprising the steps of (1) preparing a cell population cultured under conditions suitable for inducing differentiation into corneal endothelial cells, and (2) measuring the expression of at least one gene selected from the group consisting of POU6F2, LMX1B, and TFAP2B in the cell population.