公开(公告)号：US07473548B2

公开(公告)日：2009-01-06

申请号：US10423112

申请日：2003-04-25

申请人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

发明人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

IPC分类号： C12M1/34

CPC分类号： A61B5/14546 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4839 ,  A61B5/7203 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/6428 ,  G01N21/8507 ,  G01N33/86 ,  G01N2021/6415 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2021/8528

摘要： Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy at the second and third wavelengths.

摘要翻译： 基于酶的底物的切割量检测体液中酶的活化。 用两种荧光染料 - 供体和受体标记底物。 标记的基质被呈现给体液。 器件将第一波长的能量发射到体液中，并响应于第一波长的能量，检测由染料发射的第二和第三波长处的能量。 在衬底的酶切割之前，受体以响应于通过来自供体的荧光共振能量转移（FRET）接收的第二波长的能量而在第三波长处发射能量。 在酶切割底物之后，供体在第二波长处发射能量。 该装置可以基于在第二和第三波长处的能量的相对强度来确定体液内活化酶的浓度。

公开(公告)号：US08940522B2

公开(公告)日：2015-01-27

申请号：US13214860

申请日：2011-08-22

申请人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

发明人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

IPC分类号： C12M3/00 ,  A61B5/145 ,  A61B5/00 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/64 ,  G01N21/85 ,  G01N33/86

CPC分类号： A61B5/14546 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4839 ,  A61B5/7203 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/6428 ,  G01N21/8507 ,  G01N33/86 ,  G01N2021/6415 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2021/8528

摘要： Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy at the second and third wavelengths.

摘要翻译： 基于酶的底物的切割量检测体液中酶的活化。 用两种荧光染料 - 供体和受体标记底物。 标记的基质被呈现给体液。 器件将第一波长的能量发射到体液中，并响应于第一波长的能量，检测由染料发射的第二和第三波长处的能量。 在衬底的酶切割之前，受体以响应于通过来自供体的荧光共振能量转移（FRET）接收的第二波长的能量而在第三波长处发射能量。 在酶切割底物之后，供体在第二波长处发射能量。 该装置可以基于在第二和第三波长处的能量的相对强度来确定体液内活化酶的浓度。

公开(公告)号：US20120046642A1

公开(公告)日：2012-02-23

申请号：US13214860

申请日：2011-08-22

申请人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

发明人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

IPC分类号： A61M5/168

CPC分类号： A61B5/14546 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4839 ,  A61B5/7203 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/6428 ,  G01N21/8507 ,  G01N33/86 ,  G01N2021/6415 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2021/8528

摘要： Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy at the second and third wavelengths.

摘要翻译： 基于酶的底物的切割量检测体液中酶的活化。 用两种荧光染料 - 供体和受体标记底物。 标记的基质被呈现给体液。 器件将第一波长的能量发射到体液中，并响应于第一波长的能量，检测由染料发射的第二和第三波长处的能量。 在衬底的酶切割之前，受体以响应于通过来自供体的荧光共振能量转移（FRET）接收的第二波长的能量而在第三波长处发射能量。 在酶切割底物之后，供体在第二波长处发射能量。 该装置可以基于在第二和第三波长处的能量的相对强度来确定体液内活化酶的浓度。

公开(公告)号：US5775026A

公开(公告)日：1998-07-07

申请号：US624297

申请日：1996-03-29

申请人： Robert C. Pearce ,  Robby C. Murdock ,  Amy E. Thompson ,  Scott M. Reed ,  Don E. Wallace

发明人： Robert C. Pearce ,  Robby C. Murdock ,  Amy E. Thompson ,  Scott M. Reed ,  Don E. Wallace

IPC分类号： A01M1/20 ,  A01N25/18 ,  A01N43/86 ,  A01M25/00

CPC分类号： A01M1/2016 ,  A01N25/18 ,  A01N43/86 ,  A01M2200/012

摘要： A bait station for attracting and killing insects with a nithiazine-type insecticide is constructed in a substantially flat configuration, with the insecticide formulated as a solid coating on the surface of a solid sheet of moisture-free nonabsorptive material, the station further containing one or more ampoules of an attractant composition. The coated sheet and ampoules are held in a housing, with the ampoules in a compressible portion of the housing permitting them to be manually broken to discharge their contents on command, the housing containing appropriately positioned openings to both expose the insecticide surface and to permit release of the attractant vapors.

摘要翻译： 用硝基型杀虫剂吸引和杀死昆虫的诱饵站被构造成基本平坦的构型，其中杀虫剂在固体无水非吸收材料的表面上作为固体涂层配制，该站还包含一个或多个 更多安瓿的引诱剂组合物。 涂覆的片材和安瓿保持在壳体中，其中安瓿在壳体的可压缩部分中允许其被手动破碎以根据命令排出其内容物，壳体包含适当定位的开口以暴露杀虫剂表面并允许释放 的引诱蒸汽。