公开(公告)号：US10697004B2

公开(公告)日：2020-06-30

申请号：US15524656

申请日：2015-11-06

Applicant: Osaka City University

Inventor： Akira Tachibana ,  Toshizumi Tanabe

IPC: C12Q1/6827 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/574 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6851 ,  C12Q1/6883

Abstract: A method in which a mutant gene present in a gene pool mixedly with a large number of wild-type genes can be simply, inexpensively and sensitively detected is developed and provided. A clamping probe that is connected to a target nucleic acid molecule in two regions of first and second target nucleic acid complementary regions so that a wild-type target nucleic acid molecule and a mutant-type target nucleic acid molecule can be distinguished from each other depending on a difference in complementarity to these target nucleic acid molecules is provided.

公开(公告)号：US20150011745A1

公开(公告)日：2015-01-08

申请号：US14358509

申请日：2012-11-14

Applicant: Osaka City University

Inventor： Akira Tachibana ,  Toshizumi Tanabe

IPC: C12N15/113

CPC classification number: C12N15/113 ,  A61K31/711 ,  C12N2310/113 ,  C12N2310/14 ,  C12N2310/3231 ,  C12N2310/3519 ,  C12N2310/50 ,  C12N2310/531 ,  C12N2310/533 ,  C12N2320/30

Abstract: The purpose of the present invention is to develop and provide a nucleic acid molecule that can specifically and efficiently inhibit the activity of a target RNAi molecule and can be produced safely at a low cost. Provided is a nucleic acid molecule for inhibiting the activity of a target RNAi molecule. The nucleic acid molecule comprises a single-stranded nucleic acid moiety that contains one unmodified DNA region composed of a nucleotide sequence completely or sufficiently complementary to a nucleotide sequence of a functional strand having the activity in the target RNAi molecule and a double-stranded nucleic acid moiety to be linked to at least one of the 5′-end and the 3′-end of the single-stranded nucleic acid moiety.

Abstract translation: 本发明的目的是开发和提供能够特异性且有效地抑制靶RNAi分子的活性并可以以低成本安全地制备的核酸分子。 提供了用于抑制靶RNAi分子的活性的核酸分子。 核酸分子包含单链核酸部分，其含有由与靶RNAi分子中具有活性的功能链的核苷酸序列完全或充分互补的核苷酸序列构成的一个未修饰的DNA区域和双链核酸 部分连接到单链核酸部分的5'末端和3'末端中的至少一个。

公开(公告)号：US20140315982A1

公开(公告)日：2014-10-23

申请号：US14355711

申请日：2012-11-01

Applicant: OSAKA CITY UNIVERSITY

Inventor： Akira Tachibana ,  Toshizumi Tanabe

IPC: C12N15/113

CPC classification number: C12N15/113 ,  A61K31/7105 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/50 ,  C12N2320/51

Abstract: Provided are an improved double-stranded nucleic acid molecule involved in gene expression control mediated by a gene silencing mechanism, a method of producing the molecule, and a pharmaceutical composition comprising the double-stranded nucleic acid molecule. The double-stranded nucleic acid molecule for gene expression control comprises an antisense strand having a length of 18 to 28 nucleotides and a sense strand including a complementary moiety composed of a sequence sufficiently complementary to the antisense strand and a protruding single-stranded 5′-end moiety having a length of 2 to 100 nucleotides. The sense strand and the antisense strand form base pairs via the complementary moiety. The method produces such a double-stranded nucleic acid molecule, and the pharmaceutical composition contains such a double-stranded nucleic acid molecule as an active ingredient.

Abstract translation: 提供涉及由基因沉默机制介导的基因表达控制的改进的双链核酸分子，产生该分子的方法和包含双链核酸分子的药物组合物。 用于基因表达控制的双链核酸分子包含长度为18至28个核苷酸的反义链和包含由与反义链足够互补的序列构成的互补部分的有义链和突出的单链5'- 末端部分具有2至100个核苷酸的长度。 有义链和反义链通过互补部分形成碱基对。 该方法产生这样的双链核酸分子，并且该药物组合物含有作为活性成分的这种双链核酸分子。

公开(公告)号：US20160222386A1

公开(公告)日：2016-08-04

申请号：US15135856

申请日：2016-04-22

Applicant: Osaka City University

Inventor： Akira Tachibana ,  Toshizumi Tanabe

IPC: C12N15/113

CPC classification number: C12N15/113 ,  A61K31/711 ,  C12N2310/113 ,  C12N2310/14 ,  C12N2310/3231 ,  C12N2310/3519 ,  C12N2310/50 ,  C12N2310/531 ,  C12N2310/533 ,  C12N2320/30

Abstract: The purpose of the present invention is to develop and provide a nucleic acid molecule that can specifically and efficiently inhibit the activity of a target RNAi molecule and can be produced safely at a low cost. Provided is a nucleic acid molecule for inhibiting the activity of a target RNAi molecule. The nucleic acid molecule comprises a single-stranded nucleic acid moiety that contains one unmodified DNA region composed of a nucleotide sequence completely or sufficiently complementary to a nucleotide sequence of a functional strand having the activity in the target RNAi molecule and a double-stranded nucleic acid moiety to be linked to at least one of the 5′-end and the 3′-end of the single-stranded nucleic acid moiety.

公开(公告)号：US20170356033A1

公开(公告)日：2017-12-14

申请号：US15524656

申请日：2015-11-06

Applicant: Osaka City University

Inventor： Akira Tachibana ,  Toshizumi Tanabe

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6851 ,  C12Q1/6883 ,  G01N33/574

Abstract: A method in which a mutant gene present in a gene pool mixedly with a large number of wild-type genes can be simply, inexpensively and sensitively detected is developed and provided. A clamping probe that is connected to a target nucleic acid molecule in two regions of first and second target nucleic acid complementary regions so that a wild-type target nucleic acid molecule and a mutant-type target nucleic acid molecule can be distinguished from each other depending on a difference in complementarity to these target nucleic acid molecules is provided.