公开(公告)号：US4954443A

公开(公告)日：1990-09-04

申请号：US274604

申请日：1988-11-18

申请人： Osamu Yamada ,  Tadasu Fujita

发明人： Osamu Yamada ,  Tadasu Fujita

IPC分类号： C12N11/04

CPC分类号： C12N11/04 ,  Y10S530/813

摘要： Enzymes or microorganisms are immobilized by bringing a first aqueous solution into contact with a second aqueous solution containing metal ions having a valence of 3 or more. The first solution contains enzymes or microorganisms, and at least one immobilizing agent selected from the group consisting of Xanthan gum and derivatives thereof. The immobilizing agent is thereby hardened in a state to enclose the enzymes or microorganisms. Preferably, the metal ions are iron, tin, manganese or titanium ions.

摘要翻译： 通过使第一水溶液与含有3价以上的金属离子的第二水溶液接触来固定酶或微生物。 第一溶液含有酶或微生物，以及至少一种选自黄原胶及其衍生物的固定剂。 因此固定剂以包围酶或微生物的状态硬化。 优选地，金属离子是铁，锡，锰或钛离子。

公开(公告)号：US4786496A

公开(公告)日：1988-11-22

申请号：US830544

申请日：1986-02-18

申请人： Souichirou Watanabe ,  Tadasu Fujita

发明人： Souichirou Watanabe ,  Tadasu Fujita

IPC分类号： A61K31/715 ,  A61K31/195 ,  A61K36/02 ,  A61K36/05 ,  A61K38/00 ,  A61P35/00 ,  A61P37/04 ,  A61K35/80

CPC分类号： A61K36/05 ,  Y10S514/885

摘要： An immunopotentiator having an anti-tumor activity such as an effect for inhibiting the growth of tumor cells, an effect for prolonging the death by tumor, etc. is provided, which agent contains as its active ingredient, the lipid fraction or the glycolipid fraction of a marine chlorella such as Chlorella minutissima or Chlorella vulgaris, the lipid fraction being obtained e.g. by extracting a marine chlorella with an organic solvent, and the glycolipid fraction, e.g. by separating it from the lipid fraction with a mixed solvent according to silica gel chromatography.

摘要翻译： 提供具有抗肿瘤活性的免疫增强剂，例如抑制肿瘤细胞生长的作用，延长肿瘤死亡的效果等，该试剂含有作为其活性成分的脂质部分或糖脂部分 一种海洋小球藻如小球藻（Chlorella minutissima）或小球藻（Chlorella vulgaris），所获得的脂质部分例如 通过用有机溶剂萃取海洋小球藻，和糖脂部分，例如， 通过根据硅胶色谱法用混合溶剂将其与脂质部分分离。

公开(公告)号：US5569594A

公开(公告)日：1996-10-29

申请号：US438921

申请日：1995-05-10

申请人： Yuzo Ikuta ,  Satoshi Tashiro ,  Yoshiyuki Hatano ,  Tadasu Fujita ,  Noboru Andoh ,  Sachio Asaoka ,  Haruto Kobayashi ,  Takeshi Minami

发明人： Yuzo Ikuta ,  Satoshi Tashiro ,  Yoshiyuki Hatano ,  Tadasu Fujita ,  Noboru Andoh ,  Sachio Asaoka ,  Haruto Kobayashi ,  Takeshi Minami

IPC分类号： C12N9/20 ,  C12N11/08 ,  C12P7/64 ,  C12N11/06 ,  C12P7/62

CPC分类号： C12P7/6454 ,  C12N11/08 ,  C12N9/20 ,  C12P7/6481

摘要： Transesterification involving a fat, or a fat and fatty acid, or a phospholipid is carried out with a lipase or phospholipase immobilized on a polymer carrier. The transesterification is preferably carried out in a system containing a very small amount of water such as 50 to 2,000 ppm. The phospholipase can be phospholipase A.sub.2. In a first embodiment, lipase from a microorganism of the genus Rhizopus, Mucor, Alcaligenes or Candida is immobilized on the surface of a hydrophobic, insoluble organic polymer carrier having pores of an average diameter of 10 nm or larger and having on the surface epoxy groups capable of covalently binding lipase. The immobilized lipase is dried under reduced pressure. In a second embodiment, lipase is immobilized on the surface of a polymer carrier such as a hydrophobic, insoluble organic polymer carrier having a pore diameter of 5 to 1,000 nm and having on the surface a functional group capable of binding lipase and an anion-exchange group. Preferably, the functional group is an epoxy group and the anion-exchange group is a tertiary amino group. The lipase may be obtained from the same microorganism as in the first embodiment or from another lipase-producing microorganism. Iramobilization can be carried out as in the first embodiment. It is preferred to contact a solution of lipase with the polymer carrier in the presence of a fatty acid or derivative thereof for 10 minutes to 10 hours to covalently bond the lipase to the polymer carrier.

摘要翻译： 涉及脂肪或脂肪和脂肪酸或磷脂的酯交换是用固定在聚合物载体上的脂肪酶或磷脂酶进行的。 酯交换优选在含有非常少量的水如50-2000ppm的体系中进行。 磷脂酶可以是磷脂酶A2。 在第一实施方案中，将来自根霉属，毛霉属，产碱杆菌属或假丝酵母属的微生物的脂肪酶固定在具有平均直径为10nm以上的孔的疏水性不溶性有机聚合物载体的表面，并且在表面具有环氧基 能够共价结合脂肪酶。 将固定的脂肪酶在减压下干燥。 在第二实施方案中，将脂肪酶固定在聚合物载体的表面上，例如孔径为5至1000nm的疏水性不溶性有机聚合物载体，并且在表面上具有能够结合脂肪酶和阴离子交换的官能团 组。 优选地，官能团是环氧基团，阴离子交换基团是叔氨基。 脂肪酶可以从与第一实施方案相同的微生物获得，或从另一种脂肪酶生产微生物获得。 可以如第一实施方案那样进行消毒。 优选在脂肪酸或其衍生物的存在下将脂肪酶与聚合物载体接触10分钟至10小时以将脂肪酶共价结合到聚合物载体上。

公开(公告)号：US5445955A

公开(公告)日：1995-08-29

申请号：US65496

申请日：1993-05-24

申请人： Yuzo Ikuta ,  Satoshi Tashiro ,  Yoshiyuki Hatano ,  Tadasu Fujita ,  Noboru Andoh ,  Sachio Asaoka ,  Haruto Kobayashi ,  Takeshi Minami

发明人： Yuzo Ikuta ,  Satoshi Tashiro ,  Yoshiyuki Hatano ,  Tadasu Fujita ,  Noboru Andoh ,  Sachio Asaoka ,  Haruto Kobayashi ,  Takeshi Minami

IPC分类号： C12N9/20 ,  C12N11/08 ,  C12P7/64 ,  C12N11/06

CPC分类号： C12P7/6454 ,  C12N11/08 ,  C12N9/20 ,  C12P7/6481

摘要： An immobilized lipase is prepared for transesterification of oils, fats or phospholipids in a reaction system containing a very small amount of water such as 50 to 2,000 ppm. The lipase may be a phospholipase such as phospholipase A.sub.2. In a first embodiment, lipase from a microorganism of the genus Rhizopus, Mucor, Alcaligenes or Candida is immobilized on the surface of a hydrophobic, insoluble organic polymer carrier having pores of an average diameter of 10 nm or larger. A solution of lipase is contacted with the polymer carrier for 10 minutes to 40 hours to covalently bond the lipase to the carrier. The immobilized lipase is dried under reduced pressure to a water content of 0.5 to 30 wt %. In a second embodiment, lipase is immobilized on the surface of a polymer carrier such as a hydrophobic, insoluble organic polymer carrier having a pore diameter of 5 to 1,000 nm and having a functional group capable of binding lipase in a aqueous solution and an anion-exchange group on the surface. Preferably, the functional group is an epoxy group and the anion-exchange group is a tertiary amino group. The lipase may be obtained from the same microorganism as in the first embodiment or from another lipase-producing microorganism. Immobilization can be carried out as in the first embodiment. It is preferred to contact a solution of lipase with the polymer carrier in the presence of a fatty acid or derivative thereof for 10 minutes to 10 hours to covalently bond the lipase to the polymer carrier.

摘要翻译： 制备固定化的脂肪酶用于在含有非常少量的水如50-2000ppm的反应体系中对油，脂肪或磷脂进行酯交换反应。 脂肪酶可以是磷脂酶如磷脂酶A2。 在第一实施方案中，将来自根霉属，毛毛虫，产碱杆菌或假丝酵母属的微生物的脂肪酶固定在具有平均直径为10nm以上的孔的疏水性不溶性有机聚合物载体的表面。 将脂肪酶溶液与聚合物载体接触10分钟至40小时，以将脂肪酶共价键合到载体上。 将固定化的脂肪酶在减压下干燥至含水量为0.5〜30重量％。 在第二实施方案中，将脂肪酶固定在聚合物载体的表面上，例如孔径为5至1000nm的疏水性不溶性有机聚合物载体，并且具有能够在水溶液中结合脂肪酶的官能团和阴离子交换树脂， 交流组在表面上。 优选地，官能团是环氧基团，阴离子交换基团是叔氨基。 脂肪酶可以从与第一实施方案相同的微生物获得，或从另一种脂肪酶生产微生物获得。 固定可以如第一实施例那样进行。 优选在脂肪酸或其衍生物的存在下将脂肪酶与聚合物载体接触10分钟至10小时以将脂肪酶共价结合到聚合物载体上。

公开(公告)号：US5077202A

公开(公告)日：1991-12-31

申请号：US770509

申请日：1985-08-28

申请人： Akira Seto ,  Kiyohiro Kitagawa ,  Shoko Yamashita ,  Tadasu Fujita

发明人： Akira Seto ,  Kiyohiro Kitagawa ,  Shoko Yamashita ,  Tadasu Fujita

IPC分类号： A61K31/715 ,  A61K36/05 ,  C12P7/64

CPC分类号： C12P7/6472

摘要： A process for producing a glycolipid having a high eicosapentaenoic acid (EPA) content is provided, which process comprises fractionating the whole lipids of marine chlorella by means of a combination of solvent-fractionation with column chromatography, which glycolipid has platelet agglutination-inhibiting effect, serum cholesterol-increase-inhibiting effect, etc. of EPA and also having a higher absorption rate in intestinal canal than EPA triglyceride or ethyl ester.

摘要翻译： 提供了一种具有高二十碳五烯酸（EPA）含量的糖脂的制造方法，该方法包括通过溶剂分级与柱色谱法的组合分离海洋小球藻的全脂，该糖脂具有血小板凝集抑制作用， EPA的血清胆固醇增加抑制作用等，并且在肠道中的吸收率高于EPA甘油三酯或乙酯。