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    Modified Vaccinia Ankara virus variant and cultivation method
    1.
    发明授权
    Modified Vaccinia Ankara virus variant and cultivation method 有权
    改良牛痘安卡拉病毒变种及培养方法

    公开(公告)号：US08236560B2

    公开(公告)日：2012-08-07

    申请号：US13106176

    申请日：2011-05-12

    申请人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    发明人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    IPC分类号： A01N63/00

    CPC分类号： A61K39/285 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/16322

    摘要： The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost inoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera.

    摘要翻译： 本发明提供一种减毒病毒，其衍生自改良的痘苗安卡拉病毒，其特征在于丧失其在人细胞系中繁殖复制的能力。 它还描述了源自该病毒的重组病毒和病毒或其重组体作为药物或疫苗的用途。 提供了一种方法，用于在可能被免疫损害的个体中诱导免疫应答，接受抗病毒治疗或具有疫苗病毒的预先存在的免疫。 此外，提供了一种用于在痘苗病毒原/痘苗病毒加强接种方案中施用治疗有效量的病毒或其重组体的方法。 本发明涉及在无血清培养基中培养的原代细胞中的病毒扩增方法。 通过该方法产生的病毒有利地不含动物血清中包含的任何传染性物质。

    MODIFIED VACCINIA ANKARA VIRUS VARIANT AND CULTIVATION METHOD
    2.
    发明申请
    MODIFIED VACCINIA ANKARA VIRUS VARIANT AND CULTIVATION METHOD 有权
    改良的VACCINIA安卡拉病毒变种和培养方法

    公开(公告)号：US20080317778A1

    公开(公告)日：2008-12-25

    申请号：US12200176

    申请日：2008-08-28

    申请人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    发明人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    IPC分类号： A61K39/12 ,  C12N7/02

    CPC分类号： A61K39/285 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/16322

    摘要： The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost inoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera.

    摘要翻译： 本发明提供一种减毒病毒，其衍生自改良的痘苗安卡拉病毒，其特征在于丧失其在人细胞系中繁殖复制的能力。 它还描述了源自该病毒的重组病毒和病毒或其重组体作为药物或疫苗的用途。 提供了一种方法，用于在可能被免疫损害的个体中诱导免疫应答，接受抗病毒治疗或具有疫苗病毒的预先存在的免疫。 此外，提供了一种用于在痘苗病毒原/痘苗病毒加强接种方案中施用治疗有效量的病毒或其重组体的方法。 本发明涉及在无血清培养基中培养的原代细胞中的病毒扩增方法。 通过该方法产生的病毒有利地不含动物血清中包含的任何传染性物质。

    Modified vaccinia ankara virus variant and cultivation method
    5.
    发明申请
    Modified vaccinia ankara virus variant and cultivation method 有权
    改良痘苗病毒变种和培养方法

    公开(公告)号：US20050214323A1

    公开(公告)日：2005-09-29

    申请号：US11071741

    申请日：2005-03-03

    申请人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    发明人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    IPC分类号： A61K39/275 ,  A61K39/285 ,  C12N7/00 ,  C12N15/869

    CPC分类号： A61K39/285 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/16322

    摘要： The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost innoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera.

    摘要翻译： 本发明提供一种减毒病毒，其衍生自改良的痘苗安卡拉病毒，其特征在于丧失其在人细胞系中繁殖复制的能力。 它还描述了源自该病毒的重组病毒和病毒或其重组体作为药物或疫苗的用途。 提供了一种方法，用于在可能被免疫损害的个体中诱导免疫应答，接受抗病毒治疗或具有疫苗病毒的预先存在的免疫。 此外，提供了一种用于在痘苗病毒原/痘苗病毒增强接种方案中施用治疗有效量的病毒或其重组体的方法。 本发明涉及在无血清培养基中培养的原代细胞中的病毒扩增方法。 通过该方法产生的病毒有利地不含动物血清中包含的任何传染性物质。

    Modified Vaccinia Ankara virus variant and cultivation method
    6.
    发明授权
    Modified Vaccinia Ankara virus variant and cultivation method 有权
    改良牛痘安卡拉病毒变种及培养方法

    公开(公告)号：US08470598B2

    公开(公告)日：2013-06-25

    申请号：US13544201

    申请日：2012-07-09

    申请人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    发明人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    IPC分类号： A01N63/00

    CPC分类号： A61K39/285 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/16322

    摘要： The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost innoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera.

    摘要翻译： 本发明提供一种减毒病毒，其衍生自改良的痘苗安卡拉病毒，其特征在于丧失其在人细胞系中繁殖复制的能力。 它还描述了源自该病毒的重组病毒和病毒或其重组体作为药物或疫苗的用途。 提供了一种方法，用于在可能被免疫损害的个体中诱导免疫应答，接受抗病毒治疗或具有疫苗病毒的预先存在的免疫。 此外，提供了一种用于在痘苗病毒原/痘苗病毒增强接种方案中施用治疗有效量的病毒或其重组体的方法。 本发明涉及在无血清培养基中培养的原代细胞中的病毒扩增方法。 通过该方法产生的病毒有利地不含动物血清中包含的任何传染性物质。

    MODIFIED VACCINIA ANKARA VIRUS VARIANT AND CULTIVATION METHOD
    7.
    发明申请
    MODIFIED VACCINIA ANKARA VIRUS VARIANT AND CULTIVATION METHOD 有权
    改良的VACCINIA安卡拉病毒变种和培养方法

    公开(公告)号：US20090017536A1

    公开(公告)日：2009-01-15

    申请号：US12200295

    申请日：2008-08-28

    申请人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    发明人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger-Henschel ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    IPC分类号： C12N5/06

    CPC分类号： A61K39/285 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/16322

    摘要： The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost inoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera.

    摘要翻译： 本发明提供一种减毒病毒，其衍生自改良的痘苗安卡拉病毒，其特征在于丧失其在人细胞系中繁殖复制的能力。 它还描述了源自该病毒的重组病毒和病毒或其重组体作为药物或疫苗的用途。 提供了一种方法，用于在可能被免疫损害的个体中诱导免疫应答，接受抗病毒治疗或具有疫苗病毒的预先存在的免疫。 此外，提供了一种用于在痘苗病毒原/痘苗病毒加强接种方案中施用治疗有效量的病毒或其重组体的方法。 本发明涉及在无血清培养基中培养的原代细胞中的病毒扩增方法。 通过该方法产生的病毒有利地不含动物血清中包含的任何传染性物质。

    Modified Vaccinia Ankara virus variant and cultivation method
    9.
    发明授权
    Modified Vaccinia Ankara virus variant and cultivation method 有权
    改良牛痘安卡拉病毒变种及培养方法

    公开(公告)号：US07445924B2

    公开(公告)日：2008-11-04

    申请号：US11071741

    申请日：2005-03-03

    申请人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    发明人： Paul Chaplin ,  Paul Howley ,  Christine Meisinger ,  Ingmar Rathe ,  Eva Felder ,  Karl Heller

    IPC分类号： C12N7/02 ,  A61K39/285 ,  A61K39/02

    CPC分类号： A61K39/285 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/16322

    摘要： The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost innoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera.

    摘要翻译： 本发明提供一种减毒病毒，其衍生自改良的痘苗安卡拉病毒，其特征在于丧失其在人细胞系中繁殖复制的能力。 它还描述了源自该病毒的重组病毒和病毒或其重组体作为药物或疫苗的用途。 提供了一种方法，用于在可能被免疫损害的个体中诱导免疫应答，接受抗病毒治疗或具有疫苗病毒的预先存在的免疫。 此外，提供了一种用于在痘苗病毒原/痘苗病毒增强接种方案中施用治疗有效量的病毒或其重组体的方法。 本发明涉及在无血清培养基中培养的原代细胞中的病毒扩增方法。 通过该方法产生的病毒有利地不含动物血清中包含的任何传染性物质。

    Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
    10.
    发明授权
    Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions 有权
    在无血清条件下培养原代细胞和扩增病毒的方法

    公开(公告)号：US08329466B2

    公开(公告)日：2012-12-11

    申请号：US13106026

    申请日：2011-05-12

    申请人： Ingmar Rathe ,  Eva Felder ,  Karl Heller

    发明人： Ingmar Rathe ,  Eva Felder ,  Karl Heller

    IPC分类号： C12N5/07 ,  A61K39/285

    CPC分类号： C12N7/08 ,  C12N5/0606 ,  C12N5/0652 ,  C12N7/00 ,  C12N2500/24 ,  C12N2501/11 ,  C12N2510/02 ,  C12N2710/24151

    摘要： The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors.The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.

    摘要翻译： 本发明涉及一种培养原代细胞的方法。 将原代细胞在包含选自生长因子和附着因子的因子的无血清培养基中培养。 用于培养原代细胞的方法可以是用于扩增病毒如痘病毒的方法的一个步骤。 根据后一种方法，将原代细胞培养在包含选自生长因子和附着因子的因子的无血清培养基中。 然后用病毒感染细胞，将感染的细胞在无血清培养基中培养，直到产生子代病毒。