公开(公告)号：US20180104327A1

公开(公告)日：2018-04-19

申请号：US15511988

申请日：2015-09-18

申请人： Japan Science and Technology Agency

发明人： Yoshihiro KAWAOKA ,  Tokiko WATANABE ,  Eiryo KAWAKAMI ,  Shinji WATANABE

IPC分类号： A61K39/145 ,  C12N5/10 ,  C12N5/071 ,  C12N7/02 ,  C12N15/113 ,  C12N7/08

CPC分类号： A61K39/145 ,  C12N5/00 ,  C12N5/0686 ,  C12N5/10 ,  C12N7/02 ,  C12N7/08 ,  C12N15/113 ,  C12N2760/16051

摘要： The present invention provides cells which have a high ability to propagate influenza virus, are suitable for use in production of an influenza virus for preparing a vaccine, and are able to be cultured in vitro, and a method for producing an influenza virus using the cells. That is, the present invention provides cells for producing an influenza virus in which expression of one or more genes that encode proteins involved in an effect of suppressing influenza virus production in a cell is suppressed and the gene is at least one selected from the group including ACTG1 gene and the like, and a method for producing an influenza virus that includes infecting the cells for producing an influenza virus with an influenza virus and then culturing.

公开(公告)号：US20160317651A1

公开(公告)日：2016-11-03

申请号：US15213711

申请日：2016-07-19

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Feifei Tan ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/245 ,  C12N7/00

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N7/08 ,  C12N2710/16734 ,  C12N2710/16764

摘要： This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

摘要翻译： 本发明提供了一种减轻猪伪狂犬病病毒的方法，可有效和可重复地减弱猪伪狂犬病病毒。 通过使用所述方法，猪伪狂犬病病毒的减毒菌株可以为猪提供有效的免疫。

公开(公告)号：US09801936B2

公开(公告)日：2017-10-31

申请号：US15213711

申请日：2016-07-19

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Feifei Tan ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/00 ,  C12N7/00 ,  A61K39/245 ,  C12N7/08 ,  A61K39/12

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N7/08 ,  C12N2710/16734 ,  C12N2710/16764

摘要： This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

公开(公告)号：US20130089913A1

公开(公告)日：2013-04-11

申请号：US13688473

申请日：2012-11-29

申请人： BAVARIAN NORDIC A/S

发明人： INGMAR RATHE ,  EVA FELDER ,  KARL HELLER

IPC分类号： C12N7/08

CPC分类号： C12N7/08 ,  C12N5/0606 ,  C12N5/0652 ,  C12N7/00 ,  C12N2500/24 ,  C12N2501/11 ,  C12N2510/02 ,  C12N2710/24151

摘要： The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.

公开(公告)号：US20050214324A1

公开(公告)日：2005-09-29

申请号：US11071814

申请日：2005-03-03

申请人： Ingmar Rathe ,  Eva Felder ,  Karl Heller

发明人： Ingmar Rathe ,  Eva Felder ,  Karl Heller

IPC分类号： C12N7/02 ,  A61K39/275 ,  A61K39/285 ,  A61P31/12 ,  A61P37/04 ,  C12N5/0735 ,  C12N5/077 ,  C12N7/00 ,  C12N15/869 ,  C12Q1/70 ,  C12R1/93

CPC分类号： C12N7/08 ,  C12N5/0606 ,  C12N5/0652 ,  C12N7/00 ,  C12N2500/24 ,  C12N2501/11 ,  C12N2510/02 ,  C12N2710/24151

摘要： The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.

摘要翻译： 本发明涉及一种培养原代细胞的方法。 将原代细胞培养在包含选自生长因子和附着因子的因子的无血清培养基中。 用于培养原代细胞的方法可以是用于扩增病毒如痘病毒的方法的一个步骤。 根据后一种方法，将原代细胞培养在包含选自生长因子和附着因子的因子的无血清培养基中。 然后用病毒感染细胞，将感染的细胞在无血清培养基中培养，直到产生子代病毒。

公开(公告)号：US11964009B2

公开(公告)日：2024-04-23

申请号：US16453464

申请日：2019-06-26

申请人： Japan Science and Technology Agency

发明人： Yoshihiro Kawaoka ,  Tokiko Watanabe ,  Eiryo Kawakami ,  Shinji Watanabe

IPC分类号： C12N5/00 ,  A61K39/145 ,  C12N5/071 ,  C12N5/10 ,  C12N7/02 ,  C12N7/08 ,  C12N15/113

CPC分类号： A61K39/145 ,  C12N5/00 ,  C12N5/0686 ,  C12N5/10 ,  C12N7/02 ,  C12N7/08 ,  C12N15/113 ,  C12N2760/16051

摘要： The present invention provides cells which have a high ability to propagate influenza virus, are suitable for use in production of an influenza virus for preparing a vaccine, and are able to be cultured in vitro, and a method for producing an influenza virus using the cells. That is, the present invention provides cells for producing an influenza virus in which expression of one or more genes that encode proteins involved in an effect of suppressing influenza virus production in a cell is suppressed and the gene is at least one selected from the group including ACTG1 gene and the like, and a method for producing an influenza virus that includes infecting the cells for producing an influenza virus with an influenza virus and then culturing.

公开(公告)号：US20160279231A1

公开(公告)日：2016-09-29

申请号：US15030345

申请日：2015-07-06

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Feifei Tan ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/245 ,  C12N7/00

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N7/08 ,  C12N2710/16734 ,  C12N2710/16764

摘要： This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

摘要翻译： 本发明提供了一种减轻猪伪狂犬病病毒的方法，可有效和可重复地减弱猪伪狂犬病病毒。 通过使用所述方法，猪伪狂犬病病毒的减毒菌株可以为猪提供有效的免疫。

公开(公告)号：US20150306209A1

公开(公告)日：2015-10-29

申请号：US14651744

申请日：2013-12-18

申请人： INTERVET INC.

发明人： Terri L. Wasmoen ,  Nallakannu P. Lakshmanan ,  Kenneth A. Stachura ,  Jessica A. Moore ,  Thomas Clinton Gore

IPC分类号： A61K39/23 ,  C12N7/00

CPC分类号： A61K39/23 ,  C12N7/00 ,  C12N7/08 ,  C12N2750/14321 ,  C12N2750/14334

摘要： The present invention discloses an attenuated canine parvovirus. In addition, the present invention discloses isolated and/or recombinant canine parvovirus capsid proteins and the nucleic acids that encode the canine parvovirus capsid proteins. The present invention further discloses immunogenic compositions and/or vaccines comprising the attenuated canine parvovirus isolates, corresponding capsid proteins, and/or recombinant vectors which express nucleic acids that encode the canine parvovirus capsid proteins.

摘要翻译： 本发明公开了一种减毒犬细小病毒。 此外，本发明公开了分离的和/或重组犬细小病毒衣壳蛋白和编码犬细小病毒衣壳蛋白的核酸。 本发明还公开了包含减毒的犬细小病毒分离物，相应的衣壳蛋白和/或表达编码犬细小病毒衣壳蛋白的核酸的重组载体的免疫原性组合物和/或疫苗。

公开(公告)号：US08329466B2

公开(公告)日：2012-12-11

申请号：US13106026

申请日：2011-05-12

申请人： Ingmar Rathe ,  Eva Felder ,  Karl Heller

发明人： Ingmar Rathe ,  Eva Felder ,  Karl Heller

IPC分类号： C12N5/07 ,  A61K39/285

CPC分类号： C12N7/08 ,  C12N5/0606 ,  C12N5/0652 ,  C12N7/00 ,  C12N2500/24 ,  C12N2501/11 ,  C12N2510/02 ,  C12N2710/24151

摘要： The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors.The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.

摘要翻译： 本发明涉及一种培养原代细胞的方法。 将原代细胞在包含选自生长因子和附着因子的因子的无血清培养基中培养。 用于培养原代细胞的方法可以是用于扩增病毒如痘病毒的方法的一个步骤。 根据后一种方法，将原代细胞培养在包含选自生长因子和附着因子的因子的无血清培养基中。 然后用病毒感染细胞，将感染的细胞在无血清培养基中培养，直到产生子代病毒。

公开(公告)号：US20180171309A1

公开(公告)日：2018-06-21

申请号：US15532189

申请日：2015-12-03

申请人： Intervet Inc.

发明人： Jaap Kool

IPC分类号： C12N7/08 ,  C12N7/02 ,  A61K39/12 ,  C12N15/79

CPC分类号： C12N7/08 ,  A61K39/12 ,  C12N7/00 ,  C12N7/02 ,  C12N15/79 ,  C12N2330/51 ,  C12N2510/02 ,  C12N2510/04 ,  C12N2710/16034 ,  C12N2710/16351

摘要： The present invention relates to immortalised chicken embryo fibroblasts, to cell cultures comprising such immortalised cells, to vaccines comprising such cells, to methods for the propagation of avian viruses on such cells, and to methods for the preparation of such cells and such vaccines.