公开(公告)号：US06645758B1

公开(公告)日：2003-11-11

申请号：US07951616

申请日：1992-09-28

申请人： Paul Nicholas Schnipelsky ,  Leonard Joseph Seaberg ,  Charles Cullis Hinckley ,  Jeffrey Allen Wellman ,  William Harold Donish ,  John Bruce Findlay

发明人： Paul Nicholas Schnipelsky ,  Leonard Joseph Seaberg ,  Charles Cullis Hinckley ,  Jeffrey Allen Wellman ,  William Harold Donish ,  John Bruce Findlay

IPC分类号： C12M118

CPC分类号： G01N33/54313 ,  B01L3/502 ,  B01L3/50273 ,  B01L3/508 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0478 ,  B01L2400/0481 ,  G01N33/54326 ,  G01N35/0098

摘要： A cuvette and a method of use prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments in the cuvette or added after amplification. In the latter case, a check valve prevents amplified nucleic acid from being released. Transfer of liquids between compartments is achieved via the use of flexible compartment walls and an external pressure source, or via pistons that are part of the cuvette and operate on the compartments as a piston within a piston chamber.

摘要翻译： 将比色皿和使用方法防止通过PCR技术扩增的核酸被释放到大气中，同时仍然进行检测步骤以确定核酸是否存在。 检测试剂预先掺入比色皿的隔室或扩增后加入。 在后一种情况下，止回阀防止扩增的核酸被释放。 通过使用柔性隔室壁和外部压力源，或通过作为反应杯的一部分的活塞，并且在作为活塞室内的活塞的隔室上操作来实现隔室之间的液体传递。