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公开(公告)号：US20120159839A1
公开(公告)日：2012-06-28
申请号：US13333195
申请日：2011-12-21
Applicant: Perttu Koskinen , Reijo Tanner
Inventor： Perttu Koskinen , Reijo Tanner
CPC classification number: C12P7/16 , C12N9/2437 , C12N9/248 , C12P7/10 , C12P7/6463 , Y02E50/10 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02P20/125 , Y02P20/52 , Y02P20/582
Abstract: Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and/or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and/or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and/or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and/or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.
Abstract translation: 公开了包括第一和第二生物技术过程的综合过程。 第一种方法生产用于生物燃料的生物燃料和/或起始材料，并且使用能够产生酶的微生物。 第二种方法生产用于生物燃料的生物燃料和/或起始材料。 在综合过程中，培养微生物，生产生物燃料和/或生物燃料和酶的起始材料。 将微生物培养物，上清液或富含蛋白质的级分或含有催化活性酶的上清液的稀释液引入第一和/或第二过程中，或处理该方法的原料。 本发明还涉及生产的酶在生物燃料生产过程中或在其他应用中作为酶制剂或酶来源的用途。 本发明还涉及所生产的脂质和醇用作生物燃料，生物燃料的组分或用于生物燃料生产的起始材料的用途。
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公开(公告)号：US20120159840A1
公开(公告)日：2012-06-28
申请号：US13333203
申请日：2011-12-21
Applicant: Perttu Koskinen , Reijo Tanner
Inventor： Perttu Koskinen , Reijo Tanner
CPC classification number: C12P7/64 , C12N9/2434 , C12P21/02 , D21C5/005 , D21H11/20 , Y02E50/13 , Y02P20/125 , Y02P20/582 , Y02T50/678
Abstract: The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.
Abstract translation: 本发明涉及一种综合方法，其包括单细胞油生产过程和纸浆和/或造纸工业方法。 该方法包括在单细胞油生产过程中使用能够在包含来自纸浆和/或造纸工业的有机材料的培养基上培养时能够产生脂质或脂质和酶的微生物。 在单细胞油生产过程中和/或与其连接的过程中，所述微生物产生脂质或脂质和酶。 本发明还涉及在该方法中生产的脂质作为生物燃料或作为生物燃料的组分或用作生物燃料生产和使用在纸浆和/或造纸工业或其它应用中的脂质生产过程中生产的酶的原料 作为酶制剂或酶的来源。
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3.发明授权Integrated process system for single cell oil production and a pulp and/or paper industry process 有权用于单细胞油生产的综合过程系统和纸浆和/或造纸工业过程公开(公告)号：US08936655B2
公开(公告)日：2015-01-20
申请号：US13333203
申请日：2011-12-21
Applicant: Perttu Koskinen , Reijo Tanner
Inventor： Perttu Koskinen , Reijo Tanner
CPC classification number: C12P7/64 , C12N9/2434 , C12P21/02 , D21C5/005 , D21H11/20 , Y02E50/13 , Y02P20/125 , Y02P20/582 , Y02T50/678
Abstract: The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.
Abstract translation: 本发明涉及一种综合方法，其包括单细胞油生产过程和纸浆和/或造纸工业方法。 该方法包括在单细胞油生产过程中使用能够在包含来自纸浆和/或造纸工业的有机材料的培养基上培养时能够产生脂质或脂质和酶的微生物。 在单细胞油生产过程中和/或与其连接的过程中，所述微生物产生脂质或脂质和酶。 本发明还涉及在该方法中生产的脂质作为生物燃料或作为生物燃料的组分或用作生物燃料生产和使用在纸浆和/或造纸工业或其它应用中的脂质生产过程中生产的酶的原料 作为酶制剂或酶的来源。
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公开(公告)号：US09434962B2
公开(公告)日：2016-09-06
申请号：US13333195
申请日：2011-12-21
Applicant: Perttu Koskinen , Reijo Tanner
Inventor： Perttu Koskinen , Reijo Tanner
CPC classification number: C12P7/16 , C12N9/2437 , C12N9/248 , C12P7/10 , C12P7/6463 , Y02E50/10 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02P20/125 , Y02P20/52 , Y02P20/582
Abstract: Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and/or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and/or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and/or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and/or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.
Abstract translation: 公开了包括第一和第二生物技术过程的综合过程。 第一种方法生产用于生物燃料的生物燃料和/或起始材料，并且使用能够产生酶的微生物。 第二种方法生产用于生物燃料的生物燃料和/或起始材料。 在综合过程中，培养微生物，生产生物燃料和/或生物燃料和酶的起始材料。 将微生物培养物，上清液或富含蛋白质的级分或包含催化活性酶的上清液的稀释液引入第一和/或第二过程中，或处理该方法的原料。 本发明还涉及生产的酶在生物燃料生产过程中或在其他应用中作为酶制剂或酶来源的用途。 本发明还涉及所生产的脂质和醇用作生物燃料，生物燃料的组分或用于生物燃料生产的起始材料的用途。
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Patent Agency Ranking
公开(公告)号：US20110312063A1
公开(公告)日：2011-12-22
申请号：US13153118
申请日：2011-06-03
Applicant: Annika Malm , Reijo Tanner
Inventor： Annika Malm , Reijo Tanner
IPC: C12N1/12
CPC classification number: C12N1/12 , B03D1/1431 , C12N1/02
Abstract: The present invention relates to a method for collecting algae from an algae containing aqueous solution. The method comprises first, providing an organic coagulant to said solution and mixing the formed solution. Subsequently, an inert inorganic clay material is provided with mixing to the solution for coagulating said algae. The resulting solution is agitated and the algae is separated and collected from the solution.
Abstract translation: 本发明涉及从含藻水溶液中收集藻类的方法。 该方法首先为所述溶液提供有机凝结剂并混合形成的溶液。 随后，将惰性无机粘土材料与用于凝固所述藻类的溶液混合。 搅拌所得溶液，并从溶液中分离并收集藻类。
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公开(公告)号：US20120110898A1
公开(公告)日：2012-05-10
申请号：US13291306
申请日：2011-11-08
Applicant: Annika MALM , Reijo Tanner , Mervi Hujanen
Inventor： Annika MALM , Reijo Tanner , Mervi Hujanen
Abstract: A method for recovering lipids from microbial biomass, the method including providing wet microbial biomass which contains lipids to extraction without disrupting the biomass cell walls, and subsequently, extracting the wet microbial biomass with a liquid extractant at elevated temperature of at least 170° C. and elevated pressure. The combination of the temperature and pressure is such that the lipids in the cells are contacted with the extractant. Subsequently the extracted lipids are recovered from or with the extractant.
Abstract translation: 一种从微生物生物质中回收脂质的方法，该方法包括提供湿微生物生物质，其含有脂质以提取而不破坏生物质细胞壁，随后用液体萃取剂在至少170℃的升高的温度下萃取湿的微生物生物量。 并提高压力。 温度和压力的组合使得细胞中的脂质与萃取剂接触。 随后从提取剂中回收提取的脂质。
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公开(公告)号：US08845765B2
公开(公告)日：2014-09-30
申请号：US13291306
申请日：2011-11-08
Applicant: Annika Malm , Reijo Tanner , Mervi Hujanen
Inventor： Annika Malm , Reijo Tanner , Mervi Hujanen
Abstract: A method for recovering lipids from microbial biomass, the method including providing wet microbial biomass which contains lipids to extraction without disrupting the biomass cell walls, and subsequently, extracting the wet microbial biomass with a liquid extractant at elevated temperature of at least 170° C. and elevated pressure. The combination of the temperature and pressure is such that the lipids in the cells are contacted with the extractant. Subsequently the extracted lipids are recovered from or with the extractant.
Abstract translation: 一种从微生物生物质中回收脂质的方法，该方法包括提供湿微生物生物质，其含有脂质以提取而不破坏生物质细胞壁，随后用液体萃取剂在至少170℃的升高的温度下萃取湿的微生物生物量。 并提高压力。 温度和压力的组合使得细胞中的脂质与萃取剂接触。 随后从提取剂中回收提取的脂质。
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公开(公告)号：US08648210B2
公开(公告)日：2014-02-11
申请号：US13117185
申请日：2011-05-27
Applicant: Mervi Hujanen , Annika Malm , Reijo Tanner
Inventor： Mervi Hujanen , Annika Malm , Reijo Tanner
IPC: C11B3/00
Abstract: The present invention relates to a method for purification of lipid material originating from biological material. In the method the lipid material comprising acylglycerols and phosphorus impurities and at least one added nonpolar solvent and at least one added polar solvent is provided into a reaction zone whereby at least a two phase system comprising a nonpolar phase and a polar phase is formed. The phase system is heated in the closed reaction zone under mixing at a temperature from 150° C. to 300° C. and at a pressure wherein said solvents are in subcritical state, preferably of below 100 bar, dependent on the vapor pressure of the selected solvents, until the phosphorus impurity is removed from the polar phase. Subsequently, the nonpolar phase including the purified oil comprising acylglycerols is separated and recovered from said phase system.
Abstract translation: 本发明涉及一种源自生物材料的脂质物质的纯化方法。 在该方法中，将包含酰基甘油和磷杂质的脂质材料和至少一种添加的非极性溶剂和至少一种添加的极性溶剂提供到反应区中，由此形成包含非极性相和极性相的至少两相系统。 相体系在密封反应区中在150℃至300℃的温度下混合并在其中所述溶剂处于亚临界状态，优选低于100巴的压力下加热，这取决于蒸气压 选择的溶剂，直到磷杂质从极性相中除去。 随后，包括含有酰基甘油的纯化油的非极性相从所述相体系中分离回收。
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公开(公告)号：US20120116103A1
公开(公告)日：2012-05-10
申请号：US13117185
申请日：2011-05-27
Applicant: Mervi Hujanen , Annika Malm , Reijo Tanner
Inventor： Mervi Hujanen , Annika Malm , Reijo Tanner
Abstract: The present invention relates to a method for purification of lipid material originating from biological material. In the method the lipid material comprising acylglycerols and phosphorus impurities and at least one added nonpolar solvent and at least one added polar solvent is provided into a reaction zone whereby at least a two phase system comprising a nonpolar phase and a polar phase is formed. The phase system is heated in the closed reaction zone under mixing at a temperature from 150° C. to 300° C. and at a pressure wherein said solvents are in subcritical state, preferably of below 100 bar, dependent on the vapor pressure of the selected solvents, until the phosphorus impurity is removed from the polar phase. Sub-sequently, the nonpolar phase including the purified oil comprising acylglycerols is separated and recovered from said phase system.
Abstract translation: 本发明涉及一种源自生物材料的脂质物质的纯化方法。 在该方法中，将包含酰基甘油和磷杂质的脂质材料和至少一种添加的非极性溶剂和至少一种添加的极性溶剂提供到反应区中，由此形成包含非极性相和极性相的至少两相系统。 相体系在密封反应区中在150℃至300℃的温度下混合并在其中所述溶剂处于亚临界状态，优选低于100巴的压力下加热，这取决于蒸气压 选择的溶剂，直到磷杂质从极性相中除去。 接下来，包括含有酰基甘油的纯化油的非极性相被分离并从所述相体系中回收。
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