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    Integrated process system for single cell oil production and a pulp and/or paper industry process
    2.
    发明授权
    Integrated process system for single cell oil production and a pulp and/or paper industry process 有权
    用于单细胞油生产的综合过程系统和纸浆和/或造纸工业过程

    公开(公告)号：US08936655B2

    公开(公告)日：2015-01-20

    申请号：US13333203

    申请日：2011-12-21

    申请人： Perttu Koskinen ,  Reijo Tanner

    发明人： Perttu Koskinen ,  Reijo Tanner

    IPC分类号： C10L1/00 ,  C12P7/64 ,  C12N9/42 ,  C12P21/02 ,  D21C5/00 ,  D21H11/20

    CPC分类号： C12P7/64 ,  C12N9/2434 ,  C12P21/02 ,  D21C5/005 ,  D21H11/20 ,  Y02E50/13 ,  Y02P20/125 ,  Y02P20/582 ,  Y02T50/678

    摘要： The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.

    摘要翻译： 本发明涉及一种综合方法，其包括单细胞油生产过程和纸浆和/或造纸工业方法。 该方法包括在单细胞油生产过程中使用能够在包含来自纸浆和/或造纸工业的有机材料的培养基上培养时能够产生脂质或脂质和酶的微生物。 在单细胞油生产过程中和/或与其连接的过程中，所述微生物产生脂质或脂质和酶。 本发明还涉及在该方法中生产的脂质作为生物燃料或作为生物燃料的组分或用作生物燃料生产和使用在纸浆和/或造纸工业或其它应用中的脂质生产过程中生产的酶的原料 作为酶制剂或酶的来源。

    Oil Recovery Method
    3.
    发明申请
    Oil Recovery Method 有权
    油回收方法

    公开(公告)号：US20120220509A1

    公开(公告)日：2012-08-30

    申请号：US13399186

    申请日：2012-02-17

    申请人： Miia Mujunen ,  Ossi Pastinen ,  Ilkka Lehtomaki ,  Simo Laakso ,  Perttu Koskinen

    发明人： Miia Mujunen ,  Ossi Pastinen ,  Ilkka Lehtomaki ,  Simo Laakso ,  Perttu Koskinen

    IPC分类号： C11B3/16 ,  C10M101/04 ,  C10L1/188

    CPC分类号： C10G53/04 ,  B01D11/0492 ,  C02F1/26 ,  C02F1/66 ,  C10G1/04 ,  C10G17/00 ,  C10G31/09 ,  C10G53/10 ,  C10G2400/04 ,  C10G2400/10 ,  C10L1/026 ,  C10L1/08 ,  C11B1/10

    摘要： The invention relates to a method for the recovery of oil from an aqueous solution containing oil and solids, as well as to the use of products obtained by this method. The method of the invention comprises at least the following steps of: Adjusting the oil- and solids-containing aqueous solution to a pH which is acidic, separating an oil- and solids-containing fraction from the acidic aqueous solution with an separation agent which is hydrophilic or assumes a hydrophilic character in response to water, recovering the oil- and solids-containing fraction separated by means of said separation agent, extracting oil away from the solids with a water-insoluble extraction agent, and recovering the oil-containing water-insoluble extraction agent.

    摘要翻译： 本发明涉及从含有油和固体的水溶液中回收油的方法，以及使用通过该方法得到的产物。 本发明的方法至少包括以下步骤：将含油和固体的水溶液调节至酸性的pH，用含有分离剂的分离剂从酸性水溶液中分离含油和固体的级分 亲水或具有响应于水的亲水特性，回收通过所述分离剂分离的含有油和固体的馏分，用不溶于水的萃取剂将油从固体中提取出来，并回收含油的水 - 不溶性提取剂。

    Lipid Production
    4.
    发明申请
    Lipid Production 有权
    脂质生产

    公开(公告)号：US20120151833A1

    公开(公告)日：2012-06-21

    申请号：US13326589

    申请日：2011-12-15

    申请人： Suvi Myllyntausta ,  Virpi Kivinen ,  Antti Larjo ,  Tommi Aho ,  Perttu Koskinen ,  Matti Karp ,  Ville Santala

    发明人： Suvi Myllyntausta ,  Virpi Kivinen ,  Antti Larjo ,  Tommi Aho ,  Perttu Koskinen ,  Matti Karp ,  Ville Santala

    IPC分类号： C10L1/188 ,  C07C53/00 ,  C12P7/64 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C12P7/649 ,  C07K14/195 ,  C12N9/0008 ,  C12N9/20 ,  C12P7/6463 ,  Y02E50/13

    摘要： The present invention relates to a genetically modified Acinetobacter host for lipid production. The Acinetobacter host has been genetically modified to be deficient of one or more of genes A) a gene encoding fatty acyl-CoA reductase (EC1.2.1.n2), wherein said host is capable of increased production of TAGs and/or of total lipids compared to the parent host; and/or B) a gene encoding lipase (EC:3.1.1.3), a gene encoding pyruvate dehydrogenase (EC:1.2.2.2), and/or gene ACIAD 2177, or functional equivalents of any of said genes, wherein said host is capable of increased production of wax esters and/or total lipids compared to the parent host.

    摘要翻译： 本发明涉及用于脂质生产的转基因不动杆菌宿主。 不动杆菌宿主已被遗传修饰为缺失一个或多个基因A）编码脂肪酰辅酶A还原酶（EC1.2.1.n2）的基因，其中所述宿主能够增加TAG和/或总脂质的产生 与父母相比; 和/或B）编码脂肪酶的基因（EC：3.1.1.3），编码丙酮酸脱氢酶（EC：1.2.2.2）的基因和/或基因ACIAD 2177，或任何所述基因的功能等同物，其中所述宿主是 与母体宿主相比，能够增加蜡酯和/或总脂质的产生。

    Integrated Process for Producing Biofuels
    6.
    发明申请
    Integrated Process for Producing Biofuels 有权
    生物燃料生产综合过程

    公开(公告)号：US20120159839A1

    公开(公告)日：2012-06-28

    申请号：US13333195

    申请日：2011-12-21

    申请人： Perttu Koskinen ,  Reijo Tanner

    发明人： Perttu Koskinen ,  Reijo Tanner

    IPC分类号： C10L1/02 ,  C12P7/64

    CPC分类号： C12P7/16 ,  C12N9/2437 ,  C12N9/248 ,  C12P7/10 ,  C12P7/6463 ,  Y02E50/10 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/125 ,  Y02P20/52 ,  Y02P20/582

    摘要： Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and/or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and/or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and/or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and/or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.

    摘要翻译： 公开了包括第一和第二生物技术过程的综合过程。 第一种方法生产用于生物燃料的生物燃料和/或起始材料，并且使用能够产生酶的微生物。 第二种方法生产用于生物燃料的生物燃料和/或起始材料。 在综合过程中，培养微生物，生产生物燃料和/或生物燃料和酶的起始材料。 将微生物培养物，上清液或富含蛋白质的级分或含有催化活性酶的上清液的稀释液引入第一和/或第二过程中，或处理该方法的原料。 本发明还涉及生产的酶在生物燃料生产过程中或在其他应用中作为酶制剂或酶来源的用途。 本发明还涉及所生产的脂质和醇用作生物燃料，生物燃料的组分或用于生物燃料生产的起始材料的用途。

    Integrated Process System
    7.
    发明申请
    Integrated Process System 有权
    集成过程系统

    公开(公告)号：US20120159840A1

    公开(公告)日：2012-06-28

    申请号：US13333203

    申请日：2011-12-21

    申请人： Perttu Koskinen ,  Reijo Tanner

    发明人： Perttu Koskinen ,  Reijo Tanner

    IPC分类号： C10L1/00 ,  C10L1/18 ,  C12P7/64

    CPC分类号： C12P7/64 ,  C12N9/2434 ,  C12P21/02 ,  D21C5/005 ,  D21H11/20 ,  Y02E50/13 ,  Y02P20/125 ,  Y02P20/582 ,  Y02T50/678

    摘要： The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.

    摘要翻译： 本发明涉及一种综合方法，其包括单细胞油生产过程和纸浆和/或造纸工业方法。 该方法包括在单细胞油生产过程中使用能够在包含来自纸浆和/或造纸工业的有机材料的培养基上培养时能够产生脂质或脂质和酶的微生物。 在单细胞油生产过程中和/或与其连接的过程中，所述微生物产生脂质或脂质和酶。 本发明还涉及在该方法中生产的脂质作为生物燃料或作为生物燃料的组分或用作生物燃料生产和使用在纸浆和/或造纸工业或其它应用中的脂质生产过程中生产的酶的原料 作为酶制剂或酶的来源。

    Integrated process for producing biofuels
    9.
    发明授权
    Integrated process for producing biofuels 有权
    生物燃料生产综合过程

    公开(公告)号：US09434962B2

    公开(公告)日：2016-09-06

    申请号：US13333195

    申请日：2011-12-21

    申请人： Perttu Koskinen ,  Reijo Tanner

    发明人： Perttu Koskinen ,  Reijo Tanner

    IPC分类号： C12P7/64 ,  C12P7/16 ,  C12N9/42 ,  C12N9/24 ,  C12P7/10

    CPC分类号： C12P7/16 ,  C12N9/2437 ,  C12N9/248 ,  C12P7/10 ,  C12P7/6463 ,  Y02E50/10 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/125 ,  Y02P20/52 ,  Y02P20/582

    摘要： Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and/or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and/or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and/or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and/or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.

    摘要翻译： 公开了包括第一和第二生物技术过程的综合过程。 第一种方法生产用于生物燃料的生物燃料和/或起始材料，并且使用能够产生酶的微生物。 第二种方法生产用于生物燃料的生物燃料和/或起始材料。 在综合过程中，培养微生物，生产生物燃料和/或生物燃料和酶的起始材料。 将微生物培养物，上清液或富含蛋白质的级分或包含催化活性酶的上清液的稀释液引入第一和/或第二过程中，或处理该方法的原料。 本发明还涉及生产的酶在生物燃料生产过程中或在其他应用中作为酶制剂或酶来源的用途。 本发明还涉及所生产的脂质和醇用作生物燃料，生物燃料的组分或用于生物燃料生产的起始材料的用途。