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1.发明授权NUCLEOTIDE SEQUENCE COMPRISING THE GENOME OF HEPATITIS B VIRUS, NUCLEOTIDE SEQUENCE CODING THE SURFACE ANTIGEN OF THE HEPATITIS B VIRUS, VECTORS CONTAINING THE NUCLEOTIDE SEQUENCES, PROCESS FOR THE PREPARATION THEREOF, AND ANTIGEN OBTAINED THEREBY 失效包含乙型肝炎病毒基因组的核苷酸序列，核酸序列编码乙型肝炎病毒的表面抗原，含有核苷酸序列的载体，其制备方法和获得的抗原公开(公告)号：US06416996B1
公开(公告)日：2002-07-09
申请号：US08478853
申请日：1995-06-07
IPC分类号： A61K3929
CPC分类号： H05K3/3405 , A61K38/00 , A61K39/00 , C07K14/005 , C12N2730/10122 , C12Q1/706 , H05K3/3421 , H05K2201/1034 , H05K2201/10424
摘要： A process produces DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. Vectors contain the cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B. Nucleic acid of reduced size and a vector containing the nucleotide sequence of which DNA codes an immunogenic peptide sequence capable of inducing the generation of antibodies to the virus of viral hepatitis B. It comprises totally or partly the sequence of nucleotides represented in FIG. 9A. Application to the production by cloning in a bacterium of an immunogenic protein immunizing against hepatitis B, or application to the obtention of probes for the diagnosis of the presence of Dane particles in the serum.
摘要翻译： 一个过程产生对应于B型肝炎病毒DNA的DNA。 它包括在细菌中克隆后面的DNA，其在先前在聚合酶存在下通过相应的前体核苷酸修复。 载体在其基因组中含有克隆的DNA。 克隆的DNA可用作检测生物样品中特别是血液或血浆中B型肝炎病毒存在的探针。 其在细菌中的表达提供了含有具有针对乙型肝炎的疫苗接种性质的蛋白质片段的杂交蛋白质。具有减小大小的核酸和含有其核苷酸序列的载体编码能够诱导产生针对病毒的抗体的免疫原性肽序列 病毒性乙型肝炎。它全部或部分包括图1中所示的核苷酸序列。 9A。 通过克隆免疫抗乙型肝炎免疫原性蛋白的细菌或应用于获得用于诊断血清中丹烷颗粒的存在的探针来应用于生产。
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2.发明授权Process for the production of DNA comprising the genome of the hepatitis B virus and vector including it 失效用于生产包含乙型肝炎病毒基因组的DNA和包含它的载体的方法公开(公告)号：US06225458B1
公开(公告)日：2001-05-01
申请号：US08347162
申请日：1994-11-23
IPC分类号： A61K3929
CPC分类号： C07K14/005 , A61K39/00 , C12N2730/10122 , C12Q1/706
摘要： The invention relates to a process for producing DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. The invention also relates to vectors containing said cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B.
摘要翻译： 本发明涉及一种对应于乙型肝炎病毒DNA的DNA的方法。 它包括在细菌中克隆后面的DNA，其在先前在聚合酶存在下通过相应的前体核苷酸修复。 本发明还涉及在其基因组中含有所述克隆的DNA的载体。 克隆的DNA可用作检测生物样品中特别是血液或血浆中B型肝炎病毒存在的探针。 其在细菌中的表达提供了含有针对乙型肝炎疫苗接种性质的蛋白质片段的杂合蛋白质。
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3.发明授权Method for the transformation of cells, particularly eukaryotes by a DNA originating from viruses of hepatitis, more particularly from virus of a B viral hepatitis, and preparations containing the expression products of said DNAs 失效通过源自肝炎病毒的DNA特别是真核细胞转化细胞的方法，特别是来自B病毒性肝炎的病毒，以及含有所述DNA的表达产物的制剂
公开(公告)号：US5314808A
公开(公告)日：1994-05-24
申请号：US482322
申请日：1983-04-05
CPC分类号： C07K14/005 , C12N15/85 , A61K39/00 , C12N2730/10122
摘要： Method for the production of antigens vaccinating against the virus of B viral hepatitis. It consists of transforming a cell culture with a vector, more particularly a plasmid, itself containing an insertion sequence including itself at least the part of the viral DNA coding for the immunogen protein, capable of inducing in vivo antibody production active with respect to the whole virus, as well as the viral promoter under the control of which the transcription and translation of the above-said part of viral DNA is normally carried out, in particular in a host infected with the corresponding virus.
摘要翻译： 生产针对B型病毒性肝炎病毒的抗原的方法。 它包括用载体转化细胞培养物，更特别是质粒本身，其含有包含其自身的至少一部分编码免疫原蛋白的病毒DNA的插入序列，其能够引起体内抗体产生相对于整体的活性 病毒以及病毒启动子，其中病毒启动子的上述部分病毒DNA的转录和翻译通常进行，特别是在感染了相应病毒的宿主中。
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4.发明授权Method for the transformation of cells, particularly eukaryotes by a DNA originating from viruses of hepatitis, more particularly from virus of B viral hepatitis, and preparations containing the expression products of said DNAs 失效通过源自肝炎病毒的DNA特别是真核细胞转化细胞的方法，特别是来自B病毒性肝炎病毒的方法以及含有所述DNA的表达产物的制剂
公开(公告)号：US5591638A
公开(公告)日：1997-01-07
申请号：US191133
申请日：1994-01-25
申请人： Pierre Tiollais , Charles Chany , Marie-Fran.cedilla.oise Dubois , Christine Pourcel , Anne Louise
发明人： Pierre Tiollais , Charles Chany , Marie-Fran.cedilla.oise Dubois , Christine Pourcel , Anne Louise
CPC分类号： C07K14/005 , C12N15/85 , A61K39/00 , C12N2730/10122
摘要： Method for the production of antigens vaccinating against the virus of B viral hepatitis. It consists of transforming a cell culture with a vector, more particularly a plasmid, itself containing an insertion sequence including itself at least the part of the viral DNA coding for the immunogen protein, capable of inducing in vivo antibody production active with respect to the whole virus, as well as the viral promoter under the control of which the transcription and translation of the above-said part of viral DNA is normally carried out, in particular in a host infected with the corresponding virus.
摘要翻译： 生产针对B型病毒性肝炎病毒的抗原的方法。 它包括用载体转化细胞培养物，更特别是质粒本身，其含有包含其自身的至少一部分编码免疫原蛋白的病毒DNA的插入序列，其能够引起体内抗体产生相对于整体的活性 病毒以及病毒启动子，其中病毒启动子的上述部分病毒DNA的转录和翻译通常进行，特别是在感染了相应病毒的宿主中。
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