利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

30 条记录 (耗时 0.022 秒)

    NUCLEOTIDE SEQUENCE COMPRISING THE GENOME OF HEPATITIS B VIRUS, NUCLEOTIDE SEQUENCE CODING THE SURFACE ANTIGEN OF THE HEPATITIS B VIRUS, VECTORS CONTAINING THE NUCLEOTIDE SEQUENCES, PROCESS FOR THE PREPARATION THEREOF, AND ANTIGEN OBTAINED THEREBY
    1.
    发明授权
    NUCLEOTIDE SEQUENCE COMPRISING THE GENOME OF HEPATITIS B VIRUS, NUCLEOTIDE SEQUENCE CODING THE SURFACE ANTIGEN OF THE HEPATITIS B VIRUS, VECTORS CONTAINING THE NUCLEOTIDE SEQUENCES, PROCESS FOR THE PREPARATION THEREOF, AND ANTIGEN OBTAINED THEREBY 失效
    包含乙型肝炎病毒基因组的核苷酸序列，核酸序列编码乙型肝炎病毒的表面抗原，含有核苷酸序列的载体，其制备方法和获得的抗原

    公开(公告)号：US06416996B1

    公开(公告)日：2002-07-09

    申请号：US08478853

    申请日：1995-06-07

    申请人： Pierre Tiollais ,  Alex Fritsch ,  Christine Pourcel ,  Francois Galibert ,  Patrick Charnay

    发明人： Pierre Tiollais ,  Alex Fritsch ,  Christine Pourcel ,  Francois Galibert ,  Patrick Charnay

    IPC分类号： A61K3929

    CPC分类号： H05K3/3405 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N2730/10122 ,  C12Q1/706 ,  H05K3/3421 ,  H05K2201/1034 ,  H05K2201/10424

    摘要： A process produces DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. Vectors contain the cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B. Nucleic acid of reduced size and a vector containing the nucleotide sequence of which DNA codes an immunogenic peptide sequence capable of inducing the generation of antibodies to the virus of viral hepatitis B. It comprises totally or partly the sequence of nucleotides represented in FIG. 9A. Application to the production by cloning in a bacterium of an immunogenic protein immunizing against hepatitis B, or application to the obtention of probes for the diagnosis of the presence of Dane particles in the serum.

    摘要翻译： 一个过程产生对应于B型肝炎病毒DNA的DNA。 它包括在细菌中克隆后面的DNA，其在先前在聚合酶存在下通过相应的前体核苷酸修复。 载体在其基因组中含有克隆的DNA。 克隆的DNA可用作检测生物样品中特别是血液或血浆中B型肝炎病毒存在的探针。 其在细菌中的表达提供了含有具有针对乙型肝炎的疫苗接种性质的蛋白质片段的杂交蛋白质。具有减小大小的核酸和含有其核苷酸序列的载体编码能够诱导产生针对病毒的抗体的免疫原性肽序列 病毒性乙型肝炎。它全部或部分包括图1中所示的核苷酸序列。 9A。 通过克隆免疫抗乙型肝炎免疫原性蛋白的细菌或应用于获得用于诊断血清中丹烷颗粒的存在的探针来应用于生产。

    Process for the production of DNA comprising the genome of the hepatitis B virus and vector including it
    2.
    发明授权
    Process for the production of DNA comprising the genome of the hepatitis B virus and vector including it 失效
    用于生产包含乙型肝炎病毒基因组的DNA和包含它的载体的方法

    公开(公告)号：US06225458B1

    公开(公告)日：2001-05-01

    申请号：US08347162

    申请日：1994-11-23

    申请人： Pierre Tiollais ,  Alex Fritsch ,  Christine Pourcel ,  Patrick Charnay

    发明人： Pierre Tiollais ,  Alex Fritsch ,  Christine Pourcel ,  Patrick Charnay

    IPC分类号： A61K3929

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2730/10122 ,  C12Q1/706

    摘要： The invention relates to a process for producing DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. The invention also relates to vectors containing said cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B.

    摘要翻译： 本发明涉及一种对应于乙型肝炎病毒DNA的DNA的方法。 它包括在细菌中克隆后面的DNA，其在先前在聚合酶存在下通过相应的前体核苷酸修复。 本发明还涉及在其基因组中含有所述克隆的DNA的载体。 克隆的DNA可用作检测生物样品中特别是血液或血浆中B型肝炎病毒存在的探针。 其在细菌中的表达提供了含有针对乙型肝炎疫苗接种性质的蛋白质片段的杂合蛋白质。

    Nucleotide sequence comprising the genome of hepatitis B virus, nucleotide sequence coding the surface antigen of the hepatitis B virus, vectors containing the nucleotide sequences, process for the preparation thereof, and antigen obtained thereby
    5.
    发明授权
    Nucleotide sequence comprising the genome of hepatitis B virus, nucleotide sequence coding the surface antigen of the hepatitis B virus, vectors containing the nucleotide sequences, process for the preparation thereof, and antigen obtained thereby 失效
    包含乙型肝炎病毒基因组的核苷酸序列，编码乙型肝炎病毒表面抗原的核苷酸序列，含有核苷酸序列的载体，其制备方法和由此得到的抗原

    公开(公告)号：US6096879A

    公开(公告)日：2000-08-01

    申请号：US369998

    申请日：1995-01-09

    申请人： Pierre Tiollais ,  Francis Galibert ,  Patrick Charnay

    发明人： Pierre Tiollais ,  Francis Galibert ,  Patrick Charnay

    IPC分类号： A61K38/00 ,  A61K39/00 ,  A61P31/12 ,  C07K14/02 ,  C12Q1/70 ,  A61K39/29

    CPC分类号： C07K14/005 ,  C12Q1/706 ,  A61K38/00 ,  A61K39/00 ,  C12N2730/10122

    摘要： A process produces DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. Vectors contain the cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B. Nucleic acid of reduced size and a vector containing the nucleotide sequence of which DNA codes an immunogenic peptide sequence capable of inducing the generation of antibodies to the virus of viral hepatitis B. It comprises totally or partly the sequence of nucleotides represented in FIG. 9A. Application to the production by cloning in a bacterium of an immunogenic protein immunizing against hepatitis B, or application to the obtention of probes for the diagnosis of the presence of Dane particles in the serum.

    摘要翻译： 一个过程产生对应于B型肝炎病毒DNA的DNA。 它包括在细菌中克隆后面的DNA，其在先前在聚合酶存在下通过相应的前体核苷酸修复。 载体在其基因组中含有克隆的DNA。 克隆的DNA可用作检测生物样品中特别是血液或血浆中B型肝炎病毒存在的探针。 其在细菌中的表达提供了含有具有针对乙型肝炎的疫苗接种性质的蛋白质片段的杂交蛋白质。具有减小大小的核酸和含有其核苷酸序列的载体编码能够诱导产生针对病毒的抗体的免疫原性肽序列 病毒性乙型肝炎。它全部或部分包括图1中所示的核苷酸序列。 9A。 通过克隆免疫抗乙型肝炎免疫原性蛋白的细菌或应用于获得用于诊断血清中丹烷颗粒的存在的探针来应用于生产。

    Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human heptocellular carcinoma, and which is a retinoic acid receptor
    9.
    发明授权
    Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human heptocellular carcinoma, and which is a retinoic acid receptor 失效
    类固醇/甲状腺激素受体相关基因，其在人肝细胞癌中不适当地表达，并且其是视黄酸受体

    公开(公告)号：US5376530A

    公开(公告)日：1994-12-27

    申请号：US95706

    申请日：1993-07-22

    申请人： Hughes B. De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

    发明人： Hughes B. De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

    IPC分类号： C07K14/705 ,  C12Q1/68

    CPC分类号： C07K14/70567 ,  C12Q1/6876 ,  C12Q2600/158 ,  Y10S530/828

    摘要： A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all non-hepatic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences. The hap protein is a retinoic acid (RA) receptor identified as RAR-.beta.. The RAR-.beta. gene is transcriptionally up-regulated by retinoic acid (RA) and its promoter region may contain a RARE (retinoic acid responsive element).

    摘要翻译： 将以前分离的乙型肝炎病毒（HBV）整合到与肝细胞癌（HCC）连接的147bp细胞DNA片段中作为探针从人肝cDNA文库克隆相应的互补DNA。 核苷酸序列分析显示，已命名为hap的细胞基因的整体结构与DNA结合激素受体的结构相似。 7例肝癌和肝癌细胞系中有6例表达2.5 kb的hap mRNA，在正常成人和胎儿肝脏中检测不到，但存在于所有非肝脏组织中。 低严格杂交实验揭示了人类基因组中hap相关基因的存在。 克隆的DNA序列可用于制备纯hap蛋白，并用作检测和分离互补DNA和RNA序列的探针。 hap蛋白是鉴定为RAR-β的视黄酸（RA）受体。 RAR-β基因由视黄酸（RA）转录上调，其启动子区域可能含有RARE（视黄酸反应元件）。