公开(公告)号：US10732172B1

公开(公告)日：2020-08-04

申请号：US15576235

申请日：2016-05-22

Applicant: President and Fellows of Harvard College ,  Children's Medical Center Corporation

Inventor： Donald E. Ingber ,  Abhishek Jain ,  Andries D. van der Meer ,  Alan David Michelson ,  Andrew L. Frelinger, III ,  Riccardo Barrile

IPC: G01N33/50 ,  B01L3/00 ,  G01N33/86

Abstract: A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.

公开(公告)号：US11085911B2

公开(公告)日：2021-08-10

申请号：US16534255

申请日：2019-08-07

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Christopher David Hinojosa ,  Andries D. van der Meer ,  Marinke van der Helm ,  Abhishek Jain ,  Donald Elliot Ingber ,  Marjon Zamani

IPC: G01N33/483 ,  G01N33/50 ,  B01L3/00 ,  C12M1/00 ,  G01N15/08 ,  G01N15/00

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：US20200292531A1

公开(公告)日：2020-09-17

申请号：US15929387

申请日：2020-04-29

Applicant: President and Fellows of Harvard College ,  The Children's Medical Center Corporation

Inventor： Donald E. Ingber ,  Abhishek Jain ,  Andries D. van der Meer ,  Alan David Michelson ,  Andrew L. Frelinger, III ,  Riccardo Barrile

IPC: G01N33/50 ,  B01L3/00 ,  G01N33/86

Abstract: A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.

公开(公告)号：US20180024110A1

公开(公告)日：2018-01-25

申请号：US15694577

申请日：2017-09-01

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Christopher David Hinojosa ,  Andries D. van der Meer ,  Marinke van der Helm ,  Abhishek Jain ,  Donald Elliot Ingber ,  Marjon Zamani

IPC: G01N33/483 ,  G01N15/08 ,  B01L3/00

CPC classification number: G01N33/4833 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  B01L2400/084 ,  B01L2400/086 ,  G01N15/0826 ,  G01N33/50 ,  G01N33/5005 ,  G01N2015/0065

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：US20180008980A1

公开(公告)日：2018-01-11

申请号：US15694557

申请日：2017-09-01

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Christopher David Hinojosa ,  Andries D. van der Meer ,  Marinke van der Helm ,  Abhishek Jain ,  Donald Elliot Ingber ,  Marjon Zamani

IPC: B01L3/00 ,  G01N33/483 ,  G01N33/50

CPC classification number: G01N33/4833 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  B01L2400/084 ,  B01L2400/086 ,  G01N15/0826 ,  G01N33/50 ,  G01N33/5005 ,  G01N2015/0065

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.