公开(公告)号：US11371022B2

公开(公告)日：2022-06-28

申请号：US16076857

申请日：2017-02-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Tae-Eun Park ,  Anna Herland ,  Edward Anthony Fitzgerald ,  Donald Elliot Ingber

IPC: C12N5/071 ,  C12M3/06 ,  C12N5/00 ,  G01N27/403

Abstract: The invention relates to a method of creating a human blood-brain barrier (BBB) model from the differentiation of human pluripotent stem cells (hPSCs), wherein the BBB exhibits sustained transendothelial electrical resistances (TEER) over 2000 Ω·cm2 for at least 3 days after seeding.

公开(公告)号：US11085911B2

公开(公告)日：2021-08-10

申请号：US16534255

申请日：2019-08-07

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Christopher David Hinojosa ,  Andries D. van der Meer ,  Marinke van der Helm ,  Abhishek Jain ,  Donald Elliot Ingber ,  Marjon Zamani

IPC: G01N33/483 ,  G01N33/50 ,  B01L3/00 ,  C12M1/00 ,  G01N15/08 ,  G01N15/00

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：US20180080925A1

公开(公告)日：2018-03-22

申请号：US15563140

申请日：2016-03-31

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Kambez Hajipouran Benam ,  Richard Novak ,  Josiah Sliz ,  Thomas Charles Ferrante ,  Donald Elliot Ingber ,  Youngjae Choe

IPC: G01N33/50 ,  B01L3/00 ,  F04B49/06

CPC classification number: G01N33/5064 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/14 ,  B01L2300/0861 ,  B01L2400/0478 ,  B01L2400/049 ,  C12M1/16 ,  C12M3/04 ,  C12M23/16 ,  C12M25/02 ,  C12M29/10 ,  C12M35/04 ,  F04B17/03 ,  F04B19/22 ,  F04B49/06

Abstract: A microfluidic system for determining a response of cells comprises one or more fluid pumps. The one or more fluid pumps move a fluid across cells within a microfluidic device. The microfluidic device includes a microchannel at least partially defined by a surface having cells adhered thereto, a first port at one end of the microchannel, and a second port at an opposing end of the microchannel. The one or more fluid pumps move the fluid across the cells in a first direction toward the second port and then move the fluid across the cells in a second direction toward the first port.

公开(公告)号：US20180024110A1

公开(公告)日：2018-01-25

申请号：US15694577

申请日：2017-09-01

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Christopher David Hinojosa ,  Andries D. van der Meer ,  Marinke van der Helm ,  Abhishek Jain ,  Donald Elliot Ingber ,  Marjon Zamani

IPC: G01N33/483 ,  G01N15/08 ,  B01L3/00

CPC classification number: G01N33/4833 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  B01L2400/084 ,  B01L2400/086 ,  G01N15/0826 ,  G01N33/50 ,  G01N33/5005 ,  G01N2015/0065

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：US20180008980A1

公开(公告)日：2018-01-11

申请号：US15694557

申请日：2017-09-01

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Christopher David Hinojosa ,  Andries D. van der Meer ,  Marinke van der Helm ,  Abhishek Jain ,  Donald Elliot Ingber ,  Marjon Zamani

IPC: B01L3/00 ,  G01N33/483 ,  G01N33/50

CPC classification number: G01N33/4833 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  B01L2400/084 ,  B01L2400/086 ,  G01N15/0826 ,  G01N33/50 ,  G01N33/5005 ,  G01N2015/0065

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.