公开(公告)号：US20150259704A1

公开(公告)日：2015-09-17

申请号：US14681510

申请日：2015-04-08

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant Mali ,  Luhan Yang

IPC: C12N15/85

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

Abstract translation: 提供了改变真核细胞的方法，包括用编码与真核细胞的基因组DNA互补的RNA的核酸转染真核细胞，用编码与RNA相互作用并切割基因组DNA的酶的核酸转染真核细胞 以位点特异性方式，其中细胞表达RNA和酶，RNA结合互补基因组DNA，酶以位点特异性方式切割基因组DNA。

公开(公告)号：US09587252B2

公开(公告)日：2017-03-07

申请号：US14674895

申请日：2015-03-31

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Kevin Esvelt ,  Prashant Mali

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/90 ,  C12N15/85 ,  C07K2/00 ,  C12N15/11

CPC classification number: C12N15/907 ,  C07K2/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N15/85 ,  C12N2310/3519 ,  C12N2320/33 ,  C12N2320/50 ,  C12N2800/107 ,  C12N2800/40 ,  C12N2800/80 ,  C12N2810/10 ,  C12N2830/00 ,  C12N2830/34 ,  C12N2999/007 ,  C12Y301/00

Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.

Abstract translation: 提供了调节细胞中靶核酸表达的方法，包括使用多个正交的Cas9蛋白来同时且独立地调节相应的基因，或同时并且独立地编辑相应的基因。

公开(公告)号：US20160032274A1

公开(公告)日：2016-02-04

申请号：US14653144

申请日：2013-12-16

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant Mali ,  Luhan Yang

IPC: C12N15/10

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US09970024B2

公开(公告)日：2018-05-15

申请号：US14653144

申请日：2013-12-16

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant Mali ,  Luhan Yang

IPC: C12N15/63 ,  C12N15/82 ,  C07H21/04 ,  C12N15/85 ,  C12N15/10 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/87 ,  C12N9/22

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20170044569A9

公开(公告)日：2017-02-16

申请号：US14681510

申请日：2015-04-08

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant Mali ,  Luhan Yang

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

Abstract translation: 提供了改变真核细胞的方法，包括用编码与真核细胞的基因组DNA互补的RNA的核酸转染真核细胞，用编码与RNA相互作用并切割基因组DNA的酶的核酸转染真核细胞 以位点特异性方式，其中细胞表达RNA和酶，RNA结合互补基因组DNA，酶以位点特异性方式切割基因组DNA。