公开(公告)号：US09963736B2

公开(公告)日：2018-05-08

申请号：US14208460

申请日：2014-03-13

Applicant: QIAGEN Sciences, LLC

Inventor： Yexun Wang ,  John DiCarlo ,  Vikram Devgan ,  Qiong Jiang ,  Quan Peng

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2543/10 ,  C12Q2531/113 ,  C12Q2561/101

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

公开(公告)号：US10316351B2

公开(公告)日：2019-06-11

申请号：US15919640

申请日：2018-03-13

Applicant: QIAGEN Sciences, LLC

Inventor： Yexun Wang ,  John DiCarlo ,  Vikram Devgan ,  Qiong Jiang ,  Quan Peng

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6851

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

公开(公告)号：US20190249227A1

公开(公告)日：2019-08-15

申请号：US16397158

申请日：2019-04-29

Applicant: QIAGEN Sciences, LLC

Inventor： Yexun Wang ,  John DiCarlo ,  Vikram Devgan ,  Qiong Jiang ,  Quan Peng

IPC: C12Q1/6806

CPC classification number: C12Q1/6806 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2543/10 ,  C12Q2531/113 ,  C12Q2561/101

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

公开(公告)号：US20180208971A1

公开(公告)日：2018-07-26

申请号：US15919640

申请日：2018-03-13

Applicant: QIAGEN Sciences, LLC

Inventor： Yexun Wang ,  John DiCarlo ,  Vikram Devgan ,  Qiong Jiang ,  Quan Peng

IPC: C12Q1/6806 ,  C12Q1/6851

CPC classification number: C12Q1/6806 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2543/10 ,  C12Q2531/113 ,  C12Q2561/101

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

公开(公告)号：US10544447B2

公开(公告)日：2020-01-28

申请号：US16397158

申请日：2019-04-29

Applicant: QIAGEN Sciences, LLC

Inventor： Yexun Wang ,  John DiCarlo ,  Vikram Devgan ,  Qiong Jiang ,  Quan Peng

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6851

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

公开(公告)号：US20150038376A1

公开(公告)日：2015-02-05

申请号：US14384902

申请日：2013-03-15

Applicant: QIAGEN SCIENCES LLC

Inventor： Song Tian ,  Xiao Zeng ,  John Dicarlo ,  Jiaye Yu ,  Thomas J. Fahey ,  Vikram Devgan ,  George J. Quellhorst ,  Raymond K. Blanchard

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The methods provided herein use microarray data for feature selection and then use selected targets to generate industry standard qPCR arrays with new clinical sample assay data so order to build a classification model. This multi-step method overcomes the disadvantages of traditional biomarker identification.

Abstract translation: 本文提供的方法使用微阵列数据进行特征选择，然后使用选定的目标来生成具有新临床样品测定数据的工业标准qPCR阵列，以便构建分类模型。 这种多步法克服了传统生物标志物鉴定的缺点。

公开(公告)号：US20150100242A1

公开(公告)日：2015-04-09

申请号：US14384913

申请日：2013-03-15

Applicant: QIAGEN SCIENCES LLC

Inventor： Xiao Zeng ,  Song Tian ,  Jiaye Yu ,  John Dicarlo ,  George J. Quellhorst, JR. ,  Vikram Devgan

IPC: C12Q1/68 ,  G06F19/10

CPC classification number: C12Q1/686 ,  C12Q1/6811 ,  C12Q1/6851 ,  C12Q1/6883 ,  C12Q2600/158 ,  G16B99/00 ,  C12Q2537/165 ,  C12Q2531/113 ,  C12Q2545/10

Abstract: The methods provided focus on a quantitative molecular assay tools that systematically measure a set of pre-selected targets, with proper controls in a biological sample for identification of biomarkers or novel targets for a disease status. This allows for systematically maximizing the power of multivariate feature selection tools on the analysis of high-throughput screening data (such as microarray) and use of the well selected target to generate a qPCR array with tissue specific controls and qPCR controls to serve the needs of biomarker study.

Abstract translation: 所提供的方法侧重于定量分析测定工具，其系统地测量一组预先选定的靶标，在生物样品中进行适当的控制以鉴定生物标志物或用于疾病状态的新靶标。 这允许系统地最大化多变量特征选择工具对高通量筛选数据（例如微阵列）的分析的功能，以及使用选择良好的目标来生成具有组织特异性控制和qPCR控制的qPCR阵列，以满足 生物标志物研究。

公开(公告)号：US20140274734A1

公开(公告)日：2014-09-18

申请号：US14208460

申请日：2014-03-13

Applicant: QIAGEN Sciences, LLC

Inventor： Yexun Wang ,  John DiCarlo ,  Vikram Devgan ,  Qiong Jiang ,  Quan Peng

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2543/10 ,  C12Q2531/113 ,  C12Q2561/101

Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

Abstract translation: 本公开提供用于评估基因组DNA样品的质量的方法，阵列和试剂盒，特别是从福尔马林固定的石蜡包埋（FFPE）样品获得的样品。 本文提供的方法，阵列和试剂盒使用特异于生物基因组中的区域的引物对，其中得到具有相同或几乎相同的拷贝分布在多个染色体上的基因组DNA样品。