公开(公告)号：US20150126400A1

公开(公告)日：2015-05-07

申请号：US14596949

申请日：2015-01-14

Applicant: RAINDANCE TECHNOLOGIES, INC.

Inventor： Andrew Watson ,  Darren R. Link ,  Jeffrey Charles Olson ,  Michael Samuels

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1072 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2521/319 ,  C12Q2521/501 ,  C12Q2545/101

Abstract: The invention generally relates to method for screening for a condition in a subject. In certain embodiments, methods of the invention involve obtaining a pool of nucleic acids from a sample, incubating the nucleic acids with first and second sets of binders, in which the first set binds uniquely to different regions of a target nucleic acid in the pool, the second set binds uniquely to different regions of a reference nucleic acid in the pool, and the first and second sets include different detectable labels, removing unbound binders, detecting the labels, and screening for a condition based upon results of the detecting step.

Abstract translation: 本发明一般涉及用于筛选受试者的病症的方法。 在某些实施方案中，本发明的方法包括从样品中获得核酸库，将核酸与第一组和第二组结合物孵育，其中第一组与第一组结合到泳池中的靶核酸的不同区域， 第二组与泳池中的参考核酸的不同区域唯一地结合，并且第一组和第二组包括不同的可检测标记，去除未结合的结合物，检测标记，以及基于检测步骤的结果筛选病症。

公开(公告)号：US20190316119A1

公开(公告)日：2019-10-17

申请号：US16400858

申请日：2019-05-01

Applicant: Raindance Technologies, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12N15/10 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20160201125A1

公开(公告)日：2016-07-14

申请号：US14995744

申请日：2016-01-14

Applicant: Raindance Technologies, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/68

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract translation: 本发明提供条形码文件库及其制备和使用方法，包括获得多个核酸构建体，其中每个构建体包含独特的N-聚体和功能性N-聚体，并将构建体分离成流体隔室，使得每个隔室含有一个 或更多的独特构造的副本。 本发明还提供数字PCR和数字PCR中条形码库的使用方法。

公开(公告)号：US20170002400A1

公开(公告)日：2017-01-05

申请号：US15189672

申请日：2016-06-22

Applicant: Raindance Technologies, Inc.

Inventor： John Brian Hutchison ,  Jeffrey Charles Olson ,  Darren Roy Link

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2565/629

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.

Abstract translation: 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，提供了用于测定样品的核酸组成的方法。

公开(公告)号：US20160289670A1

公开(公告)日：2016-10-06

申请号：US14874553

申请日：2015-10-05

Applicant: Raindance Technologies, Inc.

Inventor： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12N15/10 ,  G01N33/543

CPC classification number: C12N15/1075 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/5436 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.