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公开(公告)号：US20220030850A1
公开(公告)日：2022-02-03
申请号：US17371811
申请日：2021-07-09
Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventor： Allison Hubel , Kathryn Lindsay Pollock
IPC: A01N1/02
Abstract: This disclosure describes a cryopreservative composition and methods for storing cells. Generally, the cryopreservative composition includes a sugar component and a sugar alcohol component, and is effective for storing and recovering cells without requiring dimethyl sulfoxide (DMSO).
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公开(公告)号：US20190269124A1
公开(公告)日：2019-09-05
申请号：US16383165
申请日：2019-04-12
Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventor： Allison Hubel , Kathryn Lindsay Pollock
IPC: A01N1/02
Abstract: This disclosure describes a cryopreservative composition and methods for storing cells. Generally, the cryopreservative composition includes a sugar component and a sugar alcohol component, and is effective for storing and recovering cells without requiring dimethyl sulfoxide (DMSO).
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公开(公告)号：US11985969B2
公开(公告)日：2024-05-21
申请号：US17371811
申请日：2021-07-09
Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventor： Allison Hubel , Kathryn Lindsay Pollock
IPC: A01N1/02
CPC classification number: A01N1/0221 , A01N1/0284
Abstract: This disclosure describes a cryopreservative composition and methods for storing cells. Generally, the cryopreservative composition includes a sugar component and a sugar alcohol component, and is effective for storing and recovering cells without requiring dimethyl sulfoxide (DMSO).
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公开(公告)号：US20170080124A1
公开(公告)日：2017-03-23
申请号：US15267504
申请日：2016-09-16
Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventor： Allison Hubel , Michael Dominick DiVito , Andreas Stein
CPC classification number: A61L27/24 , A61L27/227 , A61L27/306 , A61L27/52 , A61L2430/16 , C08L77/04
Abstract: This disclosure describes biocompatible composites and method for making the biocompatible composites. Generally, the biocompatible composite includes a fibril prepared from a biocompatible polymer and cationic component, and a uniform coating of silica-containing material.
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5.发明申请公开(公告)号：US20150111241A1
公开(公告)日：2015-04-23
申请号：US14394693
申请日：2013-04-17
Applicant: Regents of the University of Minnesota
Inventor： Allison Hubel , Jacob Hanna
IPC: C12M1/00
CPC classification number: C12M47/02 , B01L3/502715 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L3/502776 , B01L2300/0858 , B01L2300/0861 , B01L2400/0409 , C12M23/16 , C12M47/12
Abstract: An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.
Abstract translation: 一个实例包括一个用于从流体样品分离细胞的装置，包括一个集气室，其限定：位于中心的顶部入口，以及设置在入口的下方和入口侧的相应的侧部出口; 以及底部出口，其设置在所述顶部入口的下方，到所述两个或更多个出口的侧面，其中所述增压室被构造成接收通过所述入口的流体和细胞悬浮液，并将其引导到所述底部出口，以抵抗相应的侧壁延伸 在底部出口和两个或更多个侧部出口之间，并且其中两个或更多个侧部出口和底部出口中的每一个之间的距离被选择为鼓励细胞在力下通过底部出口排出。 入口侧和出口之间的横向/垂直距离可以提供侧向流体行进，从而允许重力的时间使细胞偏向出口。
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Patent Agency Ranking
公开(公告)号：US20220240499A1
公开(公告)日：2022-08-04
申请号：US17607695
申请日：2020-04-24
Applicant: Regents of the University of Minnesota
Inventor： Rui Li , Allison Hubel
Abstract: A cryopreservative composition includes a sugar component with a total concentration of sugar components in the composition of 300 mM or less; a sugar alcohol component, with a total concentration of sugar alcohol components in the composition of 2 M or less; and at least one of a polymer component and albumin, with the proviso that the composition includes less than a cryopreservative amount of dimethyl sulfoxide (DMSO). A method of cryopreserving a cell includes adding a cell to the cryopreservative composition; freezing the composition; storing the frozen composition; thawing the composition; removing the cell from the thawed composition; and culturing the cell under conditions effective for the cell to remain viable. The freezing may include cooling at a rate of 0.1° C./min to 5° C./min. The method may be performed without a washing step after thawing.
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公开(公告)号：US11096387B2
公开(公告)日：2021-08-24
申请号：US16383165
申请日：2019-04-12
Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventor： Allison Hubel , Kathryn Lindsay Pollock
IPC: A01N1/02
Abstract: This disclosure describes a cryopreservative composition and methods for storing cells. Generally, the cryopreservative composition includes a sugar component and a sugar alcohol component, and is effective for storing and recovering cells without requiring dimethyl sulfoxide (DMSO).
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公开(公告)号：US09909099B2
公开(公告)日：2018-03-06
申请号：US15383374
申请日：2016-12-19
Applicant: Regents of the University of Minnesota
Inventor： Allison Hubel , Jacob Hanna
CPC classification number: C12M47/02 , B01L3/502715 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L3/502776 , B01L2300/0858 , B01L2300/0861 , B01L2400/0409 , C12M23/16 , C12M47/12
Abstract: An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.
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公开(公告)号：US20170172138A1
公开(公告)日：2017-06-22
申请号：US15381530
申请日：2016-12-16
Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventor： Allison Hubel , Kathryn Lindsay Pollock
IPC: A01N1/02
CPC classification number: A01N1/0221 , A01N1/0284
Abstract: This disclosure describes a cryopreservative composition and methods for storing cells. Generally, the cryopreservative composition includes a sugar component and a sugar alcohol component, and is effective for storing and recovering cells without requiring dimethyl sulfoxide (DMSO).
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公开(公告)号：US10314302B2
公开(公告)日：2019-06-11
申请号：US15381530
申请日：2016-12-16
Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventor： Allison Hubel , Kathryn Lindsay Pollock
IPC: A01N1/02
Abstract: This disclosure describes a cryopreservative composition and methods for storing cells. Generally, the cryopreservative composition includes a sugar component and a sugar alcohol component, and is effective for storing and recovering cells without requiring dimethyl sulfoxide (DMSO).
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