公开(公告)号：US07651856B2

公开(公告)日：2010-01-26

申请号：US10596438

申请日：2004-11-11

申请人： Raimund Schuetze ,  Bernd Saegmueller ,  Kurt Harms ,  Yilmaz Niyaz

发明人： Raimund Schuetze ,  Bernd Saegmueller ,  Kurt Harms ,  Yilmaz Niyaz

IPC分类号： C12N5/00 ,  G01N1/00

CPC分类号： G01N1/2813 ,  G01N2001/284 ,  G01N2001/2886 ,  G01N2035/00277

摘要： To enable contactless laser microdissection in a closed container (10, 11), for example in the form of a Petri dish, it is proposed according to the invention to use a holding device which comprises a receiving portion (4) for arrangement in the container (10, 11) and a holding portion (1) for arrangement externally of the container (10, 11). The receiving portion (4) serves to receive at least one receiving means (9), which serves in turn to receive or collect at least one biological specimen obtained by laser microdissection from biological material (15) located in the container (10, 11). The holding portion (1) is coupled to the receiving portion (4) in contactless manner, for example by means of magnetic coupling, in such a way that, by moving the holding portion (1), the receiving portion (4), together with the receiving means (9) held thereby, may be positioned in the closed container (10, 11) precisely over the biological specimen in each case to be received.

摘要翻译： 为了使得非接触式激光显微切割在密闭容器（10,11）中，例如以培养皿的形式，根据本发明，提出使用一种保持装置，该保持装置包括用于配置在容器中的容纳部分（4） （10,11）和用于在容器（10,11）的外部配置的保持部分（1）。 接收部分（4）用于容纳至少一个接收装置（9），所述接收装置依次用于接收或收集由位于容器（10,11）中的生物材料（15）通过激光显微切割获得的至少一个生物样本， 。 保持部分（1）以例如通过磁耦合的非接触方式联接到接收部分（4），使得通过使保持部分（1），接收部分（4）一起移动 在其中保持的接收装置（9）可以在每种情况下精确地定位在密封容器（10,11）中以便接收。

公开(公告)号：US20080032034A1

公开(公告)日：2008-02-07

申请号：US10582832

申请日：2004-12-15

申请人： Yilmaz Niyaz ,  Karin Schutze

发明人： Yilmaz Niyaz ,  Karin Schutze

IPC分类号： G01N1/28

CPC分类号： G01N1/2813 ,  G01N2001/2833

摘要： Receiving element for receiving a specimen detached from a biological mass by means of laser radiation A receiving element (1) is provided for receiving a specimen detached from a biological mass (7) by means of laser radiation, the receiving element (1) comprising a receiving surface for receiving the specimen, the receiving surface comprising an adhesive agent (4) for enhancing the adhesion of the respective specimen to the receiving surface. The adhesive agent (4) suppresses the occurrence of electrostatic forces, acting on the specimen, in the receiving element (1), may be dissolved without damaging the specimen and/or may receive agents for further processing of the specimen. A suitable adhesive agent (4) is in particular a hydrogel such as for example agarose. Such a receiving element (1) is suitable in particular for collecting specimens catapulted out of the biological mass (7).

摘要翻译： 用于接收通过激光辐射从生物质体分离的样本的接收元件提供接收元件（1），用于通过激光辐射接收从生物质体（7）分离的样本，所述接收元件（1）包括 接收表面用于接收样品，所述接收表面包括用于增强各个样品对接收表面的粘附的粘合剂（4）。 粘合剂（4）抑制作用在接收元件（1）上的试样上的静电力的发生，可以溶解而不损伤样品和/或可以接收用于进一步处理样品的试剂。 合适的粘合剂（4）特别是水凝胶，例如琼脂糖。 这种接收元件（1）特别适用于收集从生物体（7）中弹出的标本。

公开(公告)号：US07923679B2

公开(公告)日：2011-04-12

申请号：US11916665

申请日：2006-06-01

申请人： Bernd Sägmüller ,  Yilmaz Niyaz ,  Thomas Staltmeier

发明人： Bernd Sägmüller ,  Yilmaz Niyaz ,  Thomas Staltmeier

IPC分类号： H05H3/04

CPC分类号： G01N1/2813 ,  B01L3/0241 ,  B01L3/0255 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2400/0454 ,  C12M33/04 ,  G01N2001/2833

摘要： In relation with a laser-induced transport process of an object from a carrier to a collecting device, the invention provides a collecting medium in the collecting device in a liquid state. Prior to the laser-induced transport process, the object is separated from a mass on the carrier by laser irradiation. After the laser-induced transport process, the object, thus selected and separated, is transferred together with the collecting medium to a destination, for example, a container, for further treatment. To this end, a manipulation system for liquids is provided, the system permitting manipulation of the collecting medium with the object contained therein with a high degree of reliability and a high throughput.

摘要翻译： 关于物体从载体到收集装置的激光诱导传输过程，本发明在液体状态下在收集装置中提供收集介质。 在激光传输过程之前，通过激光照射将物体与载体上的物质分离。 在激光诱导的运送过程之后，由此选择和分离的物体与收集介质一起转移到目的地，例如容器，用于进一步处理。 为此，提供了一种用于液体的操作系统，该系统允许其中容纳有物体的收集介质以高可靠性和高产量来操作。

公开(公告)号：US20090002682A1

公开(公告)日：2009-01-01

申请号：US12144144

申请日：2008-06-23

申请人： Bernd Sagmuller ,  Renate Burgemeister ,  Yilmaz Niyaz

发明人： Bernd Sagmuller ,  Renate Burgemeister ,  Yilmaz Niyaz

IPC分类号： G01N1/00

CPC分类号： G01N1/2813 ,  G01N2001/045 ,  G01N2001/284 ,  G01N2001/2886

摘要： A microdissection method for separating and taking a target zone of a biological preparation, disposed on a carrier device with a laser light-absorbent incisable layer and a carrier means, comprises cutting the preparation and the incisable layer along an edge segment of the target zone and removing of a non-excised segment of the incisable layer from the carrier means.

摘要翻译： 用于分离和取出生物制剂的靶区的显微切割方法，其设置在具有激光吸收剂切割层和载体装置的载体装置上，包括沿着靶区域的边缘段切割制剂和可切割层， 从载体装置移除可切割层的未切除部分。

公开(公告)号：US07907259B2

公开(公告)日：2011-03-15

申请号：US12144144

申请日：2008-06-23

申请人： Bernd Sägmüller ,  Renate Burgemeister ,  Yilmaz Niyaz

发明人： Bernd Sägmüller ,  Renate Burgemeister ,  Yilmaz Niyaz

IPC分类号： G01N1/00

CPC分类号： G01N1/2813 ,  G01N2001/045 ,  G01N2001/284 ,  G01N2001/2886

摘要： A microdissection method for separating and taking a target zone of a biological preparation, disposed on a carrier device with a laser light-absorbent incisable layer and a carrier means, comprises cutting the preparation and the incisable layer along an edge segment of the target zone and removing of a non-excised segment of the incisable layer from the carrier means.

摘要翻译： 用于分离和取出生物制剂的靶区的显微切割方法，其设置在具有激光吸收剂切割层和载体装置的载体装置上，包括沿着靶区域的边缘段切割制剂和可切割层， 从载体装置移除可切割层的未切除部分。

公开(公告)号：US20090045354A1

公开(公告)日：2009-02-19

申请号：US11916665

申请日：2006-06-01

申请人： Bernd Sagmuller ,  Yilmaz Niyaz ,  Thomas Staltmeier

发明人： Bernd Sagmuller ,  Yilmaz Niyaz ,  Thomas Staltmeier

IPC分类号： A61N5/00

CPC分类号： G01N1/2813 ,  B01L3/0241 ,  B01L3/0255 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2400/0454 ,  C12M33/04 ,  G01N2001/2833

摘要： In relation with a laser-induced transport process of an object from a carrier to a collecting device, the invention provides a collecting medium in the collecting device in a liquid state. Prior to the laser-induced transport process, the object is separated from a mass on the carrier by laser irradiation. After the laser-induced transport process, the object, thus selected and separated, is transferred together with the collecting medium to a destination, for example, a container, for further treatment. To this end, a manipulation system for liquids is provided, the system permitting manipulation of the collecting medium with the object contained therein with a high degree of reliability and a high throughput.

摘要翻译： 关于物体从载体到收集装置的激光诱导传输过程，本发明在液体状态下在收集装置中提供收集介质。 在激光传输过程之前，通过激光照射将物体与载体上的物质分离。 在激光诱导的运送过程之后，由此选择和分离的物体与收集介质一起转移到目的地，例如容器，用于进一步处理。 为此，提供了一种用于液体的操作系统，该系统允许其中容纳有物体的收集介质以高可靠性和高产量来操作。