公开(公告)号：US06156513A

公开(公告)日：2000-12-05

申请号：US130888

申请日：1998-08-07

申请人： Ramareddy V. Guntaka ,  Karl T. Weber ,  Attilla Kovacs ,  Jagannadhachari Kandala

发明人： Ramareddy V. Guntaka ,  Karl T. Weber ,  Attilla Kovacs ,  Jagannadhachari Kandala

IPC分类号： A61K38/00 ,  C07H21/00 ,  C07H21/04 ,  C12N15/113 ,  C12N15/63 ,  C12N15/85 ,  C12Q1/68

CPC分类号： C12N15/113 ,  C07H21/00 ,  A61K38/00 ,  C12N2310/15 ,  C12N2310/152

摘要： Oligomers which inhibit expression of a collagen gene are described. It is believed that each oligomer, when introduced into a cell, forms a transcription-inhibiting complex composed of the oligomer and the collagen-gene promoter region. The oligomer, preferably a phosphorothioate deoxyoligonucleotide or a ribonucleotide, preferably binds in the antiparallel orientation to the polypurine strand of a polypurine-polypyrimidine region of the promoter region of a mammalian .alpha.1(I) collagen gene.

摘要翻译： 描述抑制胶原基因表达的寡聚物。 据信每个寡聚体在被引入细胞时形成由低聚物和胶原 - 基因启动子区构成的转录抑制复合物。 寡聚物，优选硫代磷酸酯脱氧寡核苷酸或核糖核苷酸，优选以反向平行取向结合哺乳动物α1（I）胶原基因启动子区的聚脲 - 多嘧啶区域的聚脲链。

公开(公告)号：US5808037A

公开(公告)日：1998-09-15

申请号：US712357

申请日：1996-09-11

申请人： Ramareddy V. Guntaka ,  Karl Theodore Weber ,  Attilla Kovacs ,  Jagannadhachari Kandala

发明人： Ramareddy V. Guntaka ,  Karl Theodore Weber ,  Attilla Kovacs ,  Jagannadhachari Kandala

IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K31/70 ,  A61K31/7088 ,  A61K38/00 ,  A61K48/00 ,  A61P1/00 ,  A61P1/16 ,  A61P9/00 ,  A61P9/10 ,  A61P11/00 ,  A61P17/00 ,  A61P17/02 ,  C12N15/113 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/85

CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/15 ,  C12N2310/315 ,  C12N2310/3181

摘要： Oligomers which inhibit expression of a collagen gene are described. It is believed that each oligomer, when introduced into a cell, forms a transcription-inhibiting complex composed of the oligomer and the collagen-gene promoter region. The oligomer, preferably a phosphorothioate deoxyoligonucleotide, preferably binds in the antiparallel orientation to the polypurine strand of a polypurine-polypyrimidine region of the promoter region of a mammalian .alpha.1(I) collagen gene.

摘要翻译： 描述抑制胶原基因表达的寡聚物。 据信每个寡聚体在被引入细胞时形成由低聚物和胶原 - 基因启动子区构成的转录抑制复合物。 寡聚物，优选硫代磷酸酯脱氧寡核苷酸，优选以反向平行取向结合哺乳动物α1（I）胶原基因的启动子区的聚脲 - 多嘧啶区域的多元链。

公开(公告)号：US5780611A

公开(公告)日：1998-07-14

申请号：US802547

申请日：1997-02-18

申请人： Ramareddy V. Guntaka ,  Karl Theodore Weber ,  Attila Kovacs ,  Jagannadhachari Kandala

发明人： Ramareddy V. Guntaka ,  Karl Theodore Weber ,  Attila Kovacs ,  Jagannadhachari Kandala

IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K31/70 ,  A61K31/7088 ,  A61K38/00 ,  A61K48/00 ,  A61P1/00 ,  A61P1/16 ,  A61P9/00 ,  A61P9/10 ,  A61P11/00 ,  A61P17/00 ,  A61P17/02 ,  A61P43/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/113 ,  C12N15/85 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/15 ,  C12N2310/315 ,  C12N2310/3181

摘要： Oligomers which inhibit expression of a collagen gene are described. It is believed that each oligomer, when introduced into a cell, forms a transcription-inhibiting complex composed of the oligomer and the collagen-gene promoter region. The oligomer, preferably a phosphorothioate deoxyoligonucleotide, preferably binds in the antiparallel orientation to the polypurine strand of a polypurine-polypyrimidine region of the promoter region of a mammalian .alpha.1 (I) collagen gene.

摘要翻译： 描述抑制胶原基因表达的寡聚物。 据信每个寡聚体在被引入细胞时形成由低聚物和胶原 - 基因启动子区构成的转录抑制复合物。 寡聚物，优选硫代磷酸酯脱氧寡核苷酸，优选以反向平行取向结合哺乳动物α1（I）胶原基因的启动子区的聚脲 - 多嘧啶区域的多元链。

公开(公告)号：US4518690A

公开(公告)日：1985-05-21

申请号：US424631

申请日：1982-09-27

申请人： Ramareddy V. Guntaka

发明人： Ramareddy V. Guntaka

IPC分类号： C12N15/70 ,  C12P21/04 ,  C07H15/12 ,  C12N1/00 ,  C12N1/20 ,  C12N15/00 ,  C12P21/00 ,  C12P21/02

CPC分类号： C12N15/70

摘要： A molecule of double-stranded DNA having a length of about 35 base pairs has been removed from the first 150 base pairs at the 3'-end of Avian sarcoma viral DNA. This molecule binds to E. coli RNA polymerase and acts as a promoter of gene expression in E. coli. E. coli cells have been transformed with cloning vehicles which include this promoter DNA molecule and enhanced gene expression has been observed in the transformed cells. By including the promoter DNA molecule in cloning vehicles along with a gene associated with the production of a desired chemical product, such as proinsulin, the polypeptide A or B chains of insulin, the polypeptide portion of interferon, a growth hormone, an enzyme or an antibody, it will be possible to obtain enhanced production of such desired chemical products.

摘要翻译： 已经从禽类肉瘤病毒DNA的3'末端的前150个碱基对中去除了具有约35个碱基对长度的双链DNA分子。 该分子与大肠杆菌RNA聚合酶结合，作为大肠杆菌中基因表达的启动子。 已经用包含该启动子DNA分子的克隆载体转化了大肠杆菌细胞，并且已经在转化细胞中观察到增强的基因表达。 通过将克隆载体中的启动子DNA分子与产生所需化学产物（例如胰岛素原），胰岛素的多肽A或B链，干扰素的多肽部分，生长激素，酶或 抗体，可以获得这种所需化学产品的增强的生产。