公开(公告)号：US06586184B2

公开(公告)日：2003-07-01

申请号：US09825124

申请日：2001-04-03

申请人： Ramesh Kumar ,  Richard A. Metz

发明人： Ramesh Kumar ,  Richard A. Metz

IPC分类号： C12Q168

CPC分类号： C07H21/00 ,  C12N9/22 ,  C12N15/70

摘要： The invention is based on the discovery that recombinagenic oligonucleobases are active in prokaryotic cells that contain a strand transfer activity (RecA) and mismatch repair activity (MutS). Using this system a type of Duplex Mutational Vector termed a Heteroduplex Mutational Vector, was shown to be more active in prokaryotic cells than the types of mutational vectors heretofore tested. Further improvements in activity were obtained by replacing the tetrathymidine linker by a nuclease resistant oligonucleotide, such as tetra-2′-O-methyl-uridine, to link the two strands of the recombinagenic oligonucleobase and removing the DNA-containing intervening segment. The claims concern Duplex Mutational Vectors that contain the above improvements. In an alternative embodiment the claims concern the use of Duplex Mutational Vectors in prokaryotic cells.

公开(公告)号：US6004804A

公开(公告)日：1999-12-21

申请号：US78063

申请日：1998-05-12

申请人： Ramesh Kumar ,  Richard A. Metz

发明人： Ramesh Kumar ,  Richard A. Metz

IPC分类号： C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N9/22 ,  C12N15/70 ,  C12Q1/68 ,  C12N15/63 ,  C12N15/00 ,  C12N15/11

CPC分类号： C07H21/00 ,  C12N15/70 ,  C12N9/22

摘要： The invention is based on the discovery that Duplex Mutational Vectors are active in prokaryotic cells that contain a strand transfer activity (RecA) and mismatch repair activity (MutS). Using this system a type of Duplex Mutational Vector, termed a Non-Chimeric Mutational Vector, having no RNA:DNA hybrid-duplex, was shown to be active in prokaryotic cells if protected from 3' exonuclease attack. Such protection can be conferred by replacement of a tetrathymidine linker by a nuclease resistant oligonucleotide, such as tetra-2'-O-methyl-uridine, to link the two strands of the recombinagenic oligonucleobase. The claims concern 3'-exonuclease protected Non-Chimeric Mutational Vectors.

摘要翻译： 本发明基于双链突变载体在含有链转移活性（RecA）和错配修复活性（MutS））的原核细胞中有活性的发现。 使用该系统，如果保护免受3'核酸外切酶攻击，则表明在不具有RNA：DNA杂合双链体的非嵌合突变载体中的一种类型的双链突变载体在原核细胞中是有活性的。 这样的保护可以通过用核酸酶抗性寡核苷酸（例如四 - 2'-O-甲基 - 尿苷）替代四连体酶连接体来连接重组型寡核苷酸的两条链。 这些权利要求涉及3'-外切核酸酶保护的非嵌合突变载体。

公开(公告)号：US06211351B1

公开(公告)日：2001-04-03

申请号：US09429292

申请日：1999-10-28

申请人： Ramesh Kumar ,  Richard A. Metz

发明人： Ramesh Kumar ,  Richard A. Metz

IPC分类号： C12N1511

CPC分类号： C07H21/00 ,  C12N9/22 ,  C12N15/70

摘要： The invention is based on the discovery that recombinagenic oligonucleobases are active in prokaryotic cells that contain a strand transfer activity (RecA) and mismatch repair activity (MutS). Using this system a type of Duplex Mutational Vector termed a Heteroduplex Mutational Vector, was shown to be more active in prokaryotic cells than the types of mutational vectors heretofore tested. Further improvements in activity were obtained by replacing the tetrathymidine linker by a nuclease resistant oligonucleotide, such as tetra-2′-O-methyl-uridine, to link the two strands of the recombinagenic oligonucleobase and removing the DNA-containing intervening segment. The claims concern Duplex Mutational Vectors that contain the above improvements. In an alternative embodiment the claims concern the use of Duplex Mutational Vectors in prokaryotic cells.

摘要翻译： 本发明基于重组型寡核苷酸在含有链转移活性（RecA）和错配修复活性（MutS））的原核细胞中有活性的发现。 使用该系统，称为异源双链突变载体的一种双链突变载体被证明在原核细胞中比迄今测试的突变载体的类型更具活性。 通过用核酸酶抗性寡核苷酸（例如四 - 2'-O-甲基 - 尿苷）代替四硫氨酸接头来连接重组型寡核苷酸的两条链并除去含DNA的中间片段，获得活性的进一步改善。 这些权利要求涉及包含上述改进的双链突变载体。 在替代实施方案中，权利要求涉及在原核细胞中使用双链突变载体。

公开(公告)号：US08195702B2

公开(公告)日：2012-06-05

申请号：US11830545

申请日：2007-07-30

申请人： Shrikanth Shankar ,  Ramesh Kumar ,  Krishna Kunchithapadam ,  Ananth Raghavan ,  Dheeraj Pandey ,  Amit Ganesh ,  Joy M. Forsythe

发明人： Shrikanth Shankar ,  Ramesh Kumar ,  Krishna Kunchithapadam ,  Ananth Raghavan ,  Dheeraj Pandey ,  Amit Ganesh ,  Joy M. Forsythe

IPC分类号： G06F17/30

CPC分类号： G06F17/30336

摘要： Techniques are provided for processing a CREATE INDEX statement that avoids one or more locks on the underlying table. The processing of such a statement may be divided into a build phase and a cooperation phase. During the build phase, a journal table is created and published. A snapshot of the table is obtained after the last DML transaction (that was pending at the time the journal table was published) commits. The index is built based on the state of the table as of the snapshot. Any changes to the table while the index is built are recorded in the journal table. During the cooperation phase, the index is partially published. A subsequent DML statement determines whether any changes in the journal table affect the same data as the subsequent DML statement. If so, then those changes are applied to the index followed by the subsequent DML statement.

摘要翻译： 提供了技术来处理CREATE INDEX语句，以避免基础表上的一个或多个锁。 这种陈述的处理可分为建立阶段和合作阶段。 在构建阶段，创建并发布日志表。 该表的快照是在最后一个DML事务（在发布日志表时挂起的）提交之后获得的。 索引是基于快照的表的状态构建的。 索引构建时对表的任何更改记录在日记表中。 在合作阶段，该指数部分公布。 随后的DML语句确定日记表表中的任何更改是否会影响与后续DML语句相同的数据。 如果是这样，那么这些更改将应用​​于后面跟随的DML语句的索引。

公开(公告)号：US20060142518A1

公开(公告)日：2006-06-29

申请号：US11027605

申请日：2004-12-28

申请人： Zai-Ming Qiu ,  John Clark ,  Ramesh Kumar

发明人： Zai-Ming Qiu ,  John Clark ,  Ramesh Kumar

IPC分类号： C08F118/02

CPC分类号： C08F220/38

摘要： A copolymer comprises repeating units derived from at least one co-reactant comprising two or more mercapto functional groups, and repeating units derived from a fluoroacrylate comprising the reaction product of: (a) at least one fluorochemical alcohol represented by the formula: C4F9—X—OH wherein: R=hydrogen or an alkyl group of 1 to 4 carbon atoms, m=2 to 8, Rf═CnF2n+1, n=1 to 5, y=0 to 6, and q=1 to 8; (b) at least one unbranched symmetric diisocyanate; and (c) at least one hydroxy-terminated alkyl (meth)acrylate or 2-fluoroacrylate monomer having 2 to about 30 carbon atoms in its alkylene portion.

摘要翻译： 共聚物包含衍生自至少一种包含两个或多个巯基官能团的共反应物的重复单元和衍生自氟代丙烯酸酯的重复单元，该重复单元包含以下反应产物：（a）至少一种由下式表示的含氟醇： -line-formula description =“In-line Formulas”end =“lead”？> C 3 -X-OH <？in-line-formula description = “直链式”末端=“尾”→其中：R =氢或1至4个碳原子的烷基，m = 2至8，R f -C n = 1〜5，y = 0〜6，q = 1〜8; （b）至少一种非支化对称二异氰酸酯; 和（c）至少一种羟基封端的（甲基）丙烯酸烷基酯或其亚烷基部分具有2至约30个碳原子的2-氟丙烯酸酯单体。

公开(公告)号：US08041691B2

公开(公告)日：2011-10-18

申请号：US11830528

申请日：2007-07-30

申请人： Ananth Raghavan ,  Shrikanth Shankar ,  Ramesh Kumar

发明人： Ananth Raghavan ,  Shrikanth Shankar ,  Ramesh Kumar

IPC分类号： G06F7/00

CPC分类号： G06F17/30362

摘要： Techniques for a data definition language (DDL) statement acquiring a lock on a table in wait mode in a deadlock free manner are provided. A DDL statement specifies metadata of a table that is to be modified, whereas a data manipulation language (DML) statement specifies a table to be modified. When a DDL statement is received, a proxy statement is initiated and executed instead of first acquiring an exclusive lock on metadata of a particular table. The proxy statement is similar to a DML statement in that the proxy statement acquires a lock on the particular table. After the proxy statement has been processed, an exclusive lock on the metadata is acquired.

摘要翻译： 提供了一种数据定义语言（DDL）语句以无死锁的方式获取等待模式下的表的锁的技术。 DDL语句指定要修改的表的元数据，而数据操作语言（DML）语句指定要修改的表。 当接收到DDL语句时，将发起并执行代理语句，而不是首先获取特定表的元数据的排他锁。 代理语句类似于DML语句，因为代理语句在特定表上获取锁。 在处理代理语句之后，获取元数据的排他锁。

公开(公告)号：US20090037366A1

公开(公告)日：2009-02-05

申请号：US11830545

申请日：2007-07-30

申请人： Shrikanth Shankar ,  Ramesh Kumar ,  Krishna Kunchithapadam ,  Ananth Raghavan ,  Dheeraj Pandey ,  Amit Ganesh ,  Joy M. Forsythe

发明人： Shrikanth Shankar ,  Ramesh Kumar ,  Krishna Kunchithapadam ,  Ananth Raghavan ,  Dheeraj Pandey ,  Amit Ganesh ,  Joy M. Forsythe

IPC分类号： G06F17/30

CPC分类号： G06F17/30336

摘要： Techniques are provided for processing a CREATE INDEX statement that avoids one or more locks on the underlying table. The processing of such a statement may be divided into a build phase and a cooperation phase. During the build phase, a journal table is created and published. A snapshot of the table is obtained after the last DML transaction (that was pending at the time the journal table was published) commits. The index is built based on the state of the table as of the snapshot. Any changes to the table while the index is built are recorded in the journal table. During the cooperation phase, the index is partially published. A subsequent DML statement determines whether any changes in the journal table affect the same data as the subsequent DML statement. If so, then those changes are applied to the index followed by the subsequent DML statement.

摘要翻译： 提供了技术来处理CREATE INDEX语句，以避免基础表上的一个或多个锁。 这种陈述的处理可分为建立阶段和合作阶段。 在构建阶段，创建并发布日志表。 该表的快照是在最后一个DML事务（在发布日志表时挂起的）提交之后获得的。 索引是基于快照的表的状态构建的。 索引构建时对表的任何更改记录在日记表中。 在合作阶段，该指数部分公布。 随后的DML语句确定日记表表中的任何更改是否影响与后续DML语句相同的数据。 如果是这样，那么这些更改将应用​​于后面跟随的DML语句的索引。

公开(公告)号：US20080071021A1

公开(公告)日：2008-03-20

申请号：US11859295

申请日：2007-09-21

申请人： Zai-Ming Qiu ,  John Clark ,  Ramesh Kumar

发明人： Zai-Ming Qiu ,  John Clark ,  Ramesh Kumar

IPC分类号： C08L69/00

CPC分类号： C08F220/38

摘要： A copolymer comprises repeating units derived from at least one co-reactant comprising two or more mercapto functional groups, and repeating units derived from a fluoroacrylate comprising the reaction product of: (a) at least one fluorochemical alcohol represented by the formula: C4F9—X—OH wherein: R=hydrogen or an alkyl group of 1 to 4 carbon atoms, m=2 to 8, Rf=CnF2n+1, n=1 to 5, y=0 to 6, and q=1 to 8; (b) at least one unbranched symmetric diisocyanate; and (c) at least one hydroxy-terminated alkyl (meth)acrylate or 2-fluoroacrylate monomer having 2 to about 30 carbon atoms in its alkylene portion.

摘要翻译： 共聚物包含衍生自至少一种包含两个或多个巯基官能团的共反应物的重复单元和衍生自氟代丙烯酸酯的重复单元，该重复单元包含以下反应产物：（a）至少一种由下式表示的含氟醇： -line-formula description =“In-line Formulas”end =“lead”？> C 3 -X-OH <？in-line-formula description = “直链式”末端=“尾”→其中：R =氢或1至4个碳原子的烷基，m = 2至8，R f = C n n = 1〜5，y = 0〜6，q = 1〜8; （b）至少一种非支化对称二异氰酸酯; 和（c）至少一种羟基封端的（甲基）丙烯酸烷基酯或其亚烷基部分具有2至约30个碳原子的2-氟丙烯酸酯单体。

公开(公告)号：US20070173149A1

公开(公告)日：2007-07-26

申请号：US11688074

申请日：2007-03-19

申请人： Gregg CALDWELL ,  John Clark ,  David Kinning ,  Alan Kirk ,  Thomas Klun ,  Ramesh Kumar ,  Roger Mader ,  George Moore ,  Zai-Ming Qiu ,  Richard Ross

发明人： Gregg CALDWELL ,  John Clark ,  David Kinning ,  Alan Kirk ,  Thomas Klun ,  Ramesh Kumar ,  Roger Mader ,  George Moore ,  Zai-Ming Qiu ,  Richard Ross

IPC分类号： B32B27/04 ,  C08L31/00 ,  C08F8/30 ,  C07C271/14

CPC分类号： C08F220/38 ,  C07C271/20 ,  C07C271/28 ,  C07C311/09 ,  Y10T442/2287

摘要： Fluoroacrylates comprise the reaction product of: (a) at least one fluorochemical alcohol represented by the formula: CnF2n+1—X—OH wherein: n=1 to 4, R=hydrogen or an alkyl group of 1 to 4 carbon atoms, m=2 to 8, Rf=CnF2n+1, y=0 to 6, and q=1 to 8; (b) at least one unbranched symmetric diusocyanate; and (c) at least one hydroxy-terminated alkyl (meth)acrylate or 2-fluoro acrylate monomer having 2 to about 30 carbon atoms in its alkylene portion.

摘要翻译： 氟代丙烯酸酯包括以下反应产物：（a）至少一种由下式表示的含氟醇：<？in-line-formula description =“In-line Formulas”end =“lead”？> C

公开(公告)号：US5922854A

公开(公告)日：1999-07-13

申请号：US105989

申请日：1993-08-11

申请人： Ramesh Kumar ,  Ajay Sharma ,  Clara Paulhiac ,  Anastasia P. Khoury-Christianson ,  Sunita Midha

发明人： Ramesh Kumar ,  Ajay Sharma ,  Clara Paulhiac ,  Anastasia P. Khoury-Christianson ,  Sunita Midha

IPC分类号： A01K67/027 ,  A61K38/00 ,  C07H21/04 ,  C07K14/805 ,  C07K19/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12N15/85 ,  C12P21/02 ,  A61K35/14 ,  C07K1/00 ,  C07K14/00

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  C07K14/805 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/01 ,  A61K38/00

摘要： The present invention relates to the use of transgenic pigs for the production of human hemoglobin. The transgenic pigs of the invention may be used as an efficient and economical source of cell-free human hemoglobin that may be used for transfusions and other medical applications in humans. Also disclosed are the methods of purifying human hemoglobin from pig red cell lysate, and plasmids contained pig and human nucleic acid sequences for the production of transgenic pigs expressing human globin DNA sequences.

摘要翻译： 本发明涉及转基因猪用于生产人血红蛋白的用途。 本发明的转基因猪可以用作可用于人类输血和其他医疗应用的无细胞人血红蛋白的有效且经济的来源。 还公开了从猪红细胞裂解物纯化人血红蛋白的方法，质粒含有用于产生表达人珠蛋白DNA序列的转基因猪的猪和人核酸序列。