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公开(公告)号：US4634673A
公开(公告)日：1987-01-06
申请号：US594188
申请日：1984-03-28
CPC分类号： C12N9/92 , Y10S435/814 , Y10S435/816
摘要： The disclosure is directed to the treatment of a glucose isomerase extract with an amine having the general formula: ##STR1## An insoluble complex containing enzyme activity is formed. The insoluble enzyme complex may be resolubilized to produce a stable concentrated and purified glucose isomerase preparation.
摘要翻译： 本公开涉及用具有以下通式的胺处理葡萄糖异构酶提取物：形成含有酶活性的不溶性复合物。 不溶性酶复合物可以重新溶解以产生稳定的浓缩和纯化的葡萄糖异构酶制剂。
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公开(公告)号：US4618584A
公开(公告)日：1986-10-21
申请号：US684205
申请日：1984-12-20
IPC分类号： C12N9/92
CPC分类号： C12N9/92 , B01D61/145 , B01D2313/42 , Y10S435/814
摘要： This invention relates to a process for the production of a purified glucose isomerase enzyme which comprises contacting an enzyme extract containing glucose isomerase and impurities with a first polysulfone membrane not normally permeable to glucose isomerase, in the presence of a salt concentration capable of selectively inducing permeation of glucose isomerase through the membrane, and obtaining a glucose isomerase containing permeate.
摘要翻译： 本发明涉及一种生产纯化葡萄糖异构酶的方法，该方法包括在含有能够选择性诱导渗透的盐浓度的情况下使含有葡萄糖异构酶和杂质的酶提取物与葡萄糖异构酶通常不能通过的第一聚砜膜接触 的葡萄糖异构酶通过膜，并获得含葡萄糖异构酶的渗透物。
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公开(公告)号：US4347322A
公开(公告)日：1982-08-31
申请号：US224590
申请日：1981-01-12
CPC分类号： C12N9/2428 , C12N11/08 , C12N11/12 , C12N9/00 , C12N9/2408 , C12N9/2414 , C12N9/92 , Y10S435/815
摘要： Enzyme purification is carried out by contacting an impure liquid enzyme preparation containing enzyme and soluble impurities with an ion exchange material in a column to adsorb both the enzyme and impurities by the ion exchange material, adding an additional amount of the impure liquid enzyme preparation whereby the soluble impurities therein are preferentially adsorbed by the ion exchange material and the adsorbed enzyme is displaced from the ion exchange material to produce a purified liquid enzyme preparation containing higher enzyme activity than before purification. The purified enzyme is more highly adsorbed by ion exchange material when immobilizing the enzyme.
摘要翻译： 酶纯化通过使含有酶和可溶性杂质的不纯液体酶制剂与柱中的离子交换材料接触，通过离子交换材料吸附酶和杂质，进行附加量的不纯液体酶制剂，从而使 其中的可溶性杂质优先被离子交换材料吸附，并且吸附的酶从离子交换材料移位以产生含有比纯化之前更高的酶活性的纯化液体酶制剂。 当固定酶时，纯化的酶被离子交换材料更高度吸附。
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公开(公告)号：US4102745A
公开(公告)日：1978-07-25
申请号：US773450
申请日：1977-03-02
CPC分类号： C12P19/20 , C12N11/08 , C12N11/10 , C12N11/14 , C12N11/18 , C13K1/06 , Y10S435/813 , Y10S435/839 , Y10S435/918
摘要： Process for converting starch to dextrose wherein a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch is contacted with an enzyme system under conditions whereby substantially complete conversion of the starch to dextrose is achieved. The enzyme system comprises immobilized glucoamylase and alpha-amylase selected from the group consisting of soluble alpha-amylase, immobilized alpha-amylase and mixtures thereof.
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公开(公告)号：US4011137A
公开(公告)日：1977-03-08
申请号：US500975
申请日：1974-08-26
CPC分类号： C12P19/20 , C12N11/08 , C12N11/10 , C12N11/14 , C12N11/18 , C13K1/06 , Y10S435/813 , Y10S435/839 , Y10S435/918
摘要： Process for converting starch to dextrose wherein a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch is contacted with an enzyme system under conditions whereby substantially complete conversion of the starch to dextrose is achieved. The enzyme system comprises immobilized glucoamylase and alpha-amylase selected from the group consisting of soluble alpha-amylase, immobilized alpha-amylase and mixtures thereof.
摘要翻译： 将淀粉转化为葡萄糖的方法，其中将含有至少10％水解淀粉的部分水解淀粉溶液与酶体系接触，在此条件下实现淀粉基本上完全转化为葡萄糖。 酶系统包括固定化葡糖淀粉酶和选自可溶性α-淀粉酶，固定的α-淀粉酶及其混合物的α-淀粉酶。
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公开(公告)号：US4663288A
公开(公告)日：1987-05-05
申请号：US736921
申请日：1985-05-22
CPC分类号： C12N9/92 , C12N9/00 , Y10S435/814
摘要： The disclosure is directed to the resolubilization of an insoluble glucose isomerase-amine complex, wherein the amine has the general formula: ##STR1## The insoluble enzyme complex may be resolubilized to produce a stable concentrated and purified glucose isomerase preparation by reaction with a resolubilizing mixture comprising a cation exchange resin.
摘要翻译： 本发明涉及不溶性葡萄糖异构酶 - 胺络合物的再溶解，其中胺具有以下通式：不溶性酶复合物可以通过与再溶解混合物反应而再溶解以产生稳定的浓缩和纯化的葡萄糖异构酶制剂 包括阳离子交换树脂。
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公开(公告)号：US4610965A
公开(公告)日：1986-09-09
申请号：US684204
申请日：1984-12-20
IPC分类号： C12N9/92
CPC分类号： C12N9/92 , Y10S435/815
摘要： The present invention relates to a process for the production of a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.
摘要翻译： 本发明涉及一种生产纯化葡萄糖异构酶的方法，该方法包括将含有葡萄糖异构酶和可溶性杂质的不纯提取物与已知吸附葡萄糖异构酶的弱碱性离子交换材料接触; 加入一种浓度的第一盐溶液，除去未吸附和弱吸附的杂质，而不是葡萄糖异构酶; 并加入第二缓冲盐溶液洗脱吸附的葡萄糖异构酶。
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公开(公告)号：USRE28885E
公开(公告)日：1976-06-29
申请号：US300687
申请日：1972-10-25
申请人： William P. Cotter , Norman E. Lloyd , Charles W. Hinman, deceased , by Roy W. Deitchler, administrator
发明人： William P. Cotter , Norman E. Lloyd , Charles W. Hinman, deceased , by Roy W. Deitchler, administrator
摘要： The present invention is directed to a method for enzymatically isomerizing glucose in glucose-containing liquors. The presence of relatively small amounts of water-soluble salts of sulfurous acids during the enzymatic isomerization of glucose in glucose-containing liquors reduces color formation therein and increases the stability of the glucose-isomerizing enzyme.
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9.发明授权Method for maintaining immobilized glucose isomerase activity during continuous isomerization of glucose to fructose 失效在将葡萄糖连续异构化成果糖期间维持固定的葡萄糖异构酶活性的方法
公开(公告)号：US5177005A
公开(公告)日：1993-01-05
申请号：US762034
申请日：1991-09-16
申请人： Norman E. Lloyd , Richard L. Antrim
发明人： Norman E. Lloyd , Richard L. Antrim
摘要： This invention provides a method for the maintenance of continuous activity of an immobilized enzyme reaction by initially underloading an adsorbent with a desired enzyme, monitoring the activity of the enzyme and periodically adding fresh enzyme to maintain a constant, continuous level of activity until the maximum carrying capacity of the support is reached. The method is preferably carried out when continuously isomerizing glucose to fructose with glucose isomerase adsorbed to a weakly basic anion exchange resin.
摘要翻译： 本发明提供了一种维持固定化酶反应的连续活性的方法，其中首先使用所需酶负载吸附剂，监测酶的活性并周期性地加入新鲜酶以保持恒定的连续水平的活性，直到最大携带 达成了支持能力。 该方法优选在葡萄糖与弱碱性阴离子交换树脂吸附的葡萄糖异构酶连续异构化为果糖时进行。
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10.发明授权Process for converting liquefied starch to a mixture of glucose and fructose utilizing a multi-component immobilized enzyme system 失效将液体淀粉转化为使用多组分固定化酶体系的葡萄糖和果胶混合物的方法
公开(公告)号：US4111750A
公开(公告)日：1978-09-05
申请号：US723121
申请日：1976-09-14
申请人： William Colilla , Norman E. Lloyd
发明人： William Colilla , Norman E. Lloyd
摘要： A process is provided for converting liquefied starch to a mixture of glucose and fructose by the utilization of a multicomponent immobilized enzyme system. The enzyme system comprises immobilized glucoamylase, glucose isomerase and debranching enzyme.
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