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公开(公告)号:US5922585A
公开(公告)日:1999-07-13
申请号:US218265
申请日:1994-03-25
IPC分类号: C07K14/395 , C07K14/47 , C07K16/14 , C12N9/12 , C12N15/54 , G01N33/573 , C12N15/31
CPC分类号: C12N9/1247 , C07K14/395 , C07K14/4702 , C07K16/14 , G01N33/573
摘要: An RNA polymerase II holoenzyme that contains, in addition to RNA polymerase, a subset of the general transcription factors together with nine SRB proteins is described. This holoenzyme will selectively initiate transcription in vitro when supplemented with TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.
摘要翻译: 描述了RNA聚合酶II全酶,其除RNA聚合酶外还包含一些一般转录因子的子集以及九种SRB蛋白。 当补充有TATA结合蛋白(TBP)和因子a(TFIIE)时,该全酶将在体外选择性地启动转录。 至少部分通过与RNA聚合酶II的功能相互作用,SRB蛋白质正向和负向地起调节转录起始作用。
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公开(公告)号:US5919666A
公开(公告)日:1999-07-06
申请号:US540804
申请日:1995-10-11
IPC分类号: C07K14/395 , C07K14/47 , C07K16/14 , C12N9/12 , G01N33/573 , C12P19/34 , C07K1/00
CPC分类号: C12N9/1247 , C07K14/395 , C07K14/4702 , C07K16/14 , G01N33/573
摘要: Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory SRB proteins are described. These holoenzymes will selectively initiate transcription in vitro when supplemented with general transcription factors such as TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.
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3.
公开(公告)号:US06214588B1
公开(公告)日:2001-04-10
申请号:US08590399
申请日:1996-01-26
IPC分类号: C12P1934
CPC分类号: C12N9/1247 , C07K14/395 , C07K14/4702 , C07K16/14 , G01N33/573
摘要: Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory proteins are described. These holoenzymes selectively initiate transcription in vitro when supplemented with general transcription factors. The regulatory proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.
摘要翻译: 描述了含有RNA聚合酶II和一种或多种调节蛋白的真核RNA聚合酶II全酶。 当补充一般转录因子时,这些全酶在体外选择性启动转录。 调节蛋白质通过与RNA聚合酶II的功能相互作用,至少部分地起到调节转录起始的作用。
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4.
公开(公告)号:US6015682A
公开(公告)日:2000-01-18
申请号:US521872
申请日:1995-08-31
IPC分类号: C07K14/395 , C07K14/47 , C07K16/14 , C12N9/12 , C12N15/54 , G01N33/573 , C12Q1/48 , C07K1/00
CPC分类号: C07K14/395 , C07K14/4702 , C07K16/14 , C12N9/1247 , G01N33/573
摘要: Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory SRB proteins are described. These holoenzymes will selectively initiate transcription in vitro when supplemented with general transcription factors such as TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.
摘要翻译: 描述了含有RNA聚合酶II和一种或多种调节性SRB蛋白质的真核RNA聚合酶II全酶。 当补充有一般转录因子如TATA结合蛋白(TBP)和因子a(TFIIE))时,这些全息酶将选择性地启动体外转录。 至少部分通过与RNA聚合酶II的功能相互作用,SRB蛋白质正向和负向地起调节转录起始作用。
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