-
公开(公告)号:US6004779A
公开(公告)日:1999-12-21
申请号:US138024
申请日:1998-08-21
申请人: John D. Bradley , Craig M. Thompson , Jeffrey B. Moore , C. Richard Wobbe , Judith M. Healy , Caroline E. Donnelly
发明人: John D. Bradley , Craig M. Thompson , Jeffrey B. Moore , C. Richard Wobbe , Judith M. Healy , Caroline E. Donnelly
CPC分类号: C07K14/395 , C12N15/81
摘要: The invention provides novel yeast cells comprising genes whose expression can be modulated by growth in the presence or absence of metal ions, methods for making such yeast cells, and methods of using such yeast cells for determining the requirement for expression of particular genes for the growth or viability of the yeast cells.
-
公开(公告)号:US5922585A
公开(公告)日:1999-07-13
申请号:US218265
申请日:1994-03-25
IPC分类号: C07K14/395 , C07K14/47 , C07K16/14 , C12N9/12 , C12N15/54 , G01N33/573 , C12N15/31
CPC分类号: C12N9/1247 , C07K14/395 , C07K14/4702 , C07K16/14 , G01N33/573
摘要: An RNA polymerase II holoenzyme that contains, in addition to RNA polymerase, a subset of the general transcription factors together with nine SRB proteins is described. This holoenzyme will selectively initiate transcription in vitro when supplemented with TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.
摘要翻译: 描述了RNA聚合酶II全酶,其除RNA聚合酶外还包含一些一般转录因子的子集以及九种SRB蛋白。 当补充有TATA结合蛋白(TBP)和因子a(TFIIE)时,该全酶将在体外选择性地启动转录。 至少部分通过与RNA聚合酶II的功能相互作用,SRB蛋白质正向和负向地起调节转录起始作用。
-
公开(公告)号:US06841652B2
公开(公告)日:2005-01-11
申请号:US10095340
申请日:2002-03-08
申请人: John W. Connell , Joseph G. Smith, Jr. , Paul M. Hergenrother , Kent A. Watson , Craig M. Thompson
发明人: John W. Connell , Joseph G. Smith, Jr. , Paul M. Hergenrother , Kent A. Watson , Craig M. Thompson
CPC分类号: C08G73/1071 , C08G73/10 , C08G73/1014 , C08G73/1039 , C08G73/1057 , C08G73/1067
摘要: Polyimides displaying low color in thin films, atomic oxygen resistance, vacuum ultraviolet radiation resistance, solubility in organic solvents in the imide form, high glass transition (Tg) temperatures, and high thermal stability are provided. The poly(amide acid)s, copoly(amide acid)s, polyimides and copolyimides are prepared by the reaction of stoichiometric ratios of an aromatic dianhydride with diamines which contain phenylphosphine oxide groups in polar aprotic solvents. Controlled molecular weight oligomeric (amide acid)s and imides can be prepared by offsetting the stoichiometry according to the Carothers equation using excess diamine and endcapping with aromatic anhydrides The polyimide materials can be processed into various material forms such as thin films, fibers, foams, threads, adhesive film, coatings, dry powders, and fiber coated prepreg, and uses include thin film membranes on antennas, second-surface mirrors, thermal optical coatings, and multi-layer thermal insulation (MLI) blanket materials.
摘要翻译: 提供在薄膜中显示低色的聚酰亚胺,原子氧电阻,真空紫外线辐射抗性,在酰亚胺形式的有机溶剂中的溶解度,高玻璃化转变温度和高热稳定性。 聚(酰胺酸),共聚(酰胺酸),聚酰亚胺和共聚酰亚胺通过芳族二酐与极性非质子溶剂中含有苯基氧化膦基团的二胺的化学计量比的反应来制备。 控制分子量低聚(酰胺酸)和酰亚胺可以通过使用过量的二胺和用芳族酸酐封端根据Carothers方程来抵消化学计量来制备。聚酰亚胺材料可以加工成各种材料形式,例如薄膜,纤维,泡沫, 螺纹,粘合剂膜,涂料,干粉和纤维涂层预浸料,并且包括天线上的薄膜膜,第二表面反射镜,热光学涂层和多层保温(MLI)覆盖材料。
-
公开(公告)号:US06531289B1
公开(公告)日:2003-03-11
申请号:US09573322
申请日:2000-05-18
IPC分类号: C12N1581
CPC分类号: C07K14/395 , C12N15/81 , C12Q1/18
摘要: The invention provides novel yeast cells comprising genes whose expression can be modulated by growth in the presence or absence of metal ions, methods for making such yeast cells, and methods of using such yeast cells for determining the requirement for expression of particular genes for the growth or viability of the yeast cells. The invention also provides methods of using such yeast cells in the isolation, screening and analysis of candidate antifungal compounds.
摘要翻译: 本发明提供了包含可在金属离子存在或不存在下通过生长调节表达的基因的新型酵母细胞,以及用于制备这种酵母细胞的方法,以及使用这种酵母细胞确定特定生长基因表达需求的方法 或酵母细胞的活力。 本发明还提供了在候选抗真菌化合物的分离,筛选和分析中使用这种酵母细胞的方法。
-
公开(公告)号:US5919666A
公开(公告)日:1999-07-06
申请号:US540804
申请日:1995-10-11
IPC分类号: C07K14/395 , C07K14/47 , C07K16/14 , C12N9/12 , G01N33/573 , C12P19/34 , C07K1/00
CPC分类号: C12N9/1247 , C07K14/395 , C07K14/4702 , C07K16/14 , G01N33/573
摘要: Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory SRB proteins are described. These holoenzymes will selectively initiate transcription in vitro when supplemented with general transcription factors such as TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.
-
公开(公告)号:US06365409B1
公开(公告)日:2002-04-02
申请号:US09404066
申请日:1999-09-23
申请人: John D. Bradley , Craig M. Thompson , Jeffrey B. Moore , C. Richard Wobbe , Judith M. Healy , Caroline E. Donnelly
发明人: John D. Bradley , Craig M. Thompson , Jeffrey B. Moore , C. Richard Wobbe , Judith M. Healy , Caroline E. Donnelly
IPC分类号: C12N1574
CPC分类号: C07K14/395 , C12N15/81
摘要: The invention provides novel yeast cells comprising genes whose expression can be modulated by growth in the presence or absence of metal ions, methods for making such yeast cells, and methods of using such yeast cells for determining the requirement for expression of particular genes for the growth or viability of the yeast cells.
摘要翻译: 本发明提供了包含可在金属离子存在或不存在下通过生长调节表达的基因的新型酵母细胞,以及用于制备这种酵母细胞的方法,以及使用这种酵母细胞确定特定生长基因表达需求的方法 或酵母细胞的活力。
-
公开(公告)号:US06358708B1
公开(公告)日:2002-03-19
申请号:US09495066
申请日:2000-01-31
申请人: Craig M. Thompson
发明人: Craig M. Thompson
IPC分类号: C12P2102
CPC分类号: C07K14/40
摘要: Disclosed herein is a purified isolated nucleic acid encoding Candida Albicans SRB-7 (CaSRB-7) and an isolated polypeptide encoded by said nucleic acid. Also disclosed herein are vectors comprising the nucleic acid sequences, cells comprising the vectors, methods for producing the polypeptides and methods of use thereof.
摘要翻译: 本文公开了编码白色念珠菌SRB-7(CaSRB-7)的纯化分离的核酸和由所述核酸编码的分离的多肽。 本文还公开了包含核酸序列的载体,包含载体的细胞,用于产生多肽的方法及其使用方法。
-
公开(公告)号:US07109287B2
公开(公告)日:2006-09-19
申请号:US10988407
申请日:2004-11-08
申请人: John W. Connell , Joseph G. Smith, Jr. , Paul M. Hergenrother , Kent A. Watson , Craig M. Thompson
发明人: John W. Connell , Joseph G. Smith, Jr. , Paul M. Hergenrother , Kent A. Watson , Craig M. Thompson
IPC分类号: C08G69/26
CPC分类号: C08G73/1071 , C08G73/10 , C08G73/1014 , C08G73/1039 , C08G73/1057 , C08G73/1067
摘要: Polyimides displaying low color in thin films, atomic oxygen resistance, vacuum ultraviolet radiation resistance, solubility in organic solvents in the imide form, high glass transition (Tg) temperatures, and high thermal stability are provided. The poly(amide acid)s, copoly(amide acid)s, polyimides and copolyimides are prepared by the reaction of stoichiometric ratios of an aromatic dianhydride with diamines which contain phenylphosphine oxide groups in polar aprotic solvents. Controlled molecular weight oligomeric (amide acid)s and imides can be prepared by offsetting the stoichiometry according to the Carothers equation using excess diamine and endcapping with aromatic anhydrides. The polyimide materials can be processed into various material forms such as thin films, fibers, foams, threads, adhesive film, coatings, dry powders, and fiber coated prepreg, and uses include thin film membranes on antennas, second-surface mirrors, thermal optical coatings, and multi-layer thermal insulation (MLI) blanket materials.
摘要翻译: 提供在薄膜中显示低色的聚酰亚胺,原子氧电阻,真空紫外线辐射抗性,在酰亚胺形式的有机溶剂中的溶解度,高玻璃化转变温度和高热稳定性。 聚(酰胺酸),共聚(酰胺酸),聚酰亚胺和共聚酰亚胺通过芳族二酐与极性非质子溶剂中含有苯基氧化膦基团的二胺的化学计量比的反应来制备。 控制分子量的低聚(酰胺酸)和酰亚胺可以通过使用过量的二胺和用芳族酸酐封端根据Carothers方程来抵消化学计量来制备。 聚酰亚胺材料可以加工成各种材料形式,如薄膜,纤维,泡沫,线,粘合膜,涂料,干粉和纤维涂层预浸料,其中包括天线薄膜,第二面反光镜,热光学 涂层和多层保温(MLI)覆层材料。
-
9.
公开(公告)号:US06214588B1
公开(公告)日:2001-04-10
申请号:US08590399
申请日:1996-01-26
IPC分类号: C12P1934
CPC分类号: C12N9/1247 , C07K14/395 , C07K14/4702 , C07K16/14 , G01N33/573
摘要: Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory proteins are described. These holoenzymes selectively initiate transcription in vitro when supplemented with general transcription factors. The regulatory proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.
摘要翻译: 描述了含有RNA聚合酶II和一种或多种调节蛋白的真核RNA聚合酶II全酶。 当补充一般转录因子时,这些全酶在体外选择性启动转录。 调节蛋白质通过与RNA聚合酶II的功能相互作用,至少部分地起到调节转录起始的作用。
-
10.
公开(公告)号:US6015682A
公开(公告)日:2000-01-18
申请号:US521872
申请日:1995-08-31
IPC分类号: C07K14/395 , C07K14/47 , C07K16/14 , C12N9/12 , C12N15/54 , G01N33/573 , C12Q1/48 , C07K1/00
CPC分类号: C07K14/395 , C07K14/4702 , C07K16/14 , C12N9/1247 , G01N33/573
摘要: Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory SRB proteins are described. These holoenzymes will selectively initiate transcription in vitro when supplemented with general transcription factors such as TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.
摘要翻译: 描述了含有RNA聚合酶II和一种或多种调节性SRB蛋白质的真核RNA聚合酶II全酶。 当补充有一般转录因子如TATA结合蛋白(TBP)和因子a(TFIIE))时,这些全息酶将选择性地启动体外转录。 至少部分通过与RNA聚合酶II的功能相互作用,SRB蛋白质正向和负向地起调节转录起始作用。
-
-
-
-
-
-
-
-
-