公开(公告)号：US06214588B1

公开(公告)日：2001-04-10

申请号：US08590399

申请日：1996-01-26

申请人： Richard A. Young ,  Anthony J. Koleske ,  Craig M. Thompson ,  David M. Chao

发明人： Richard A. Young ,  Anthony J. Koleske ,  Craig M. Thompson ,  David M. Chao

IPC分类号： C12P1934

CPC分类号： C12N9/1247 ,  C07K14/395 ,  C07K14/4702 ,  C07K16/14 ,  G01N33/573

摘要： Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory proteins are described. These holoenzymes selectively initiate transcription in vitro when supplemented with general transcription factors. The regulatory proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.

摘要翻译： 描述了含有RNA聚合酶II和一种或多种调节蛋白的真核RNA聚合酶II全酶。 当补充一般转录因子时，这些全酶在体外选择性启动转录。 调节蛋白质通过与RNA聚合酶II的功能相互作用，至少部分地起到调节转录起始的作用。

公开(公告)号：US6015682A

公开(公告)日：2000-01-18

申请号：US521872

申请日：1995-08-31

申请人： Richard A. Young ,  Anthony J. Koleske ,  Craig M. Thompson ,  David M. Chao

发明人： Richard A. Young ,  Anthony J. Koleske ,  Craig M. Thompson ,  David M. Chao

IPC分类号： C07K14/395 ,  C07K14/47 ,  C07K16/14 ,  C12N9/12 ,  C12N15/54 ,  G01N33/573 ,  C12Q1/48 ,  C07K1/00

CPC分类号： C07K14/395 ,  C07K14/4702 ,  C07K16/14 ,  C12N9/1247 ,  G01N33/573

摘要： Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory SRB proteins are described. These holoenzymes will selectively initiate transcription in vitro when supplemented with general transcription factors such as TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.

摘要翻译： 描述了含有RNA聚合酶II和一种或多种调节性SRB蛋白质的真核RNA聚合酶II全酶。 当补充有一般转录因子如TATA结合蛋白（TBP）和因子a（TFIIE））时，这些全息酶将选择性地启动体外转录。 至少部分通过与RNA聚合酶II的功能相互作用，SRB蛋白质正向和负向地起调节转录起始作用。

公开(公告)号：US5922585A

公开(公告)日：1999-07-13

申请号：US218265

申请日：1994-03-25

申请人： Richard A. Young ,  Anthony J. Koleske ,  Craig M. Thompson

发明人： Richard A. Young ,  Anthony J. Koleske ,  Craig M. Thompson

IPC分类号： C07K14/395 ,  C07K14/47 ,  C07K16/14 ,  C12N9/12 ,  C12N15/54 ,  G01N33/573 ,  C12N15/31

CPC分类号： C12N9/1247 ,  C07K14/395 ,  C07K14/4702 ,  C07K16/14 ,  G01N33/573

摘要： An RNA polymerase II holoenzyme that contains, in addition to RNA polymerase, a subset of the general transcription factors together with nine SRB proteins is described. This holoenzyme will selectively initiate transcription in vitro when supplemented with TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.

摘要翻译： 描述了RNA聚合酶II全酶，其除RNA聚合酶外还包含一些一般转录因子的子集以及九种SRB蛋白。 当补充有TATA结合蛋白（TBP）和因子a（TFIIE）时，该全酶将在体外选择性地启动转录。 至少部分通过与RNA聚合酶II的功能相互作用，SRB蛋白质正向和负向地起调节转录起始作用。

公开(公告)号：US5919666A

公开(公告)日：1999-07-06

申请号：US540804

申请日：1995-10-11

申请人： Richard A. Young ,  Anthony J. Koleske ,  Craig M. Thompson ,  David Min Chao

发明人： Richard A. Young ,  Anthony J. Koleske ,  Craig M. Thompson ,  David Min Chao

IPC分类号： C07K14/395 ,  C07K14/47 ,  C07K16/14 ,  C12N9/12 ,  G01N33/573 ,  C12P19/34 ,  C07K1/00

CPC分类号： C12N9/1247 ,  C07K14/395 ,  C07K14/4702 ,  C07K16/14 ,  G01N33/573

摘要： Eukaryotic RNA polymerase II holoenzymes that contain RNA polymerase II and one or more regulatory SRB proteins are described. These holoenzymes will selectively initiate transcription in vitro when supplemented with general transcription factors such as TATA-binding protein (TBP) and factor a (TFIIE). The SRB proteins act positively and negatively to regulate transcription initiation, at least in part, via functional interactions with RNA polymerase II.

公开(公告)号：US20090221428A1

公开(公告)日：2009-09-03

申请号：US12065076

申请日：2006-09-07

申请人： Richard A. Young ,  Tong Ihn Lee ,  Matthew Guenther ,  Laurie A. Boyer

发明人： Richard A. Young ,  Tong Ihn Lee ,  Matthew Guenther ,  Laurie A. Boyer

IPC分类号： C40B20/04 ,  C12Q1/68 ,  C07H1/00 ,  C40B50/14

CPC分类号： C12Q1/6809 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2523/101 ,  C12Q2522/101

摘要： The invention relates to improved methods of identifying the genomic regions to which a protein of interest binds, and in particular, to methods that apply to stem cells such as but not limited to; embryonic stem cells and adult stem cells. The invention also provides methods of identifying agents which modulate differentiation of stem cells. The invention also provides methods of defining the differentiation potential of a cell and of designing array oligonucleotides.

摘要翻译： 本发明涉及鉴定目标蛋白质结合的基因组区域的改进方法，特别涉及适用于干细胞的方法，例如但不限于： 胚胎干细胞和成体干细胞。 本发明还提供鉴定调节干细胞分化的药剂的方法。 本发明还提供了定义细胞的分化潜能和设计阵列寡核苷酸的方法。

公开(公告)号：US06653071B1

公开(公告)日：2003-11-25

申请号：US09326137

申请日：1999-06-04

申请人： Frank C. P. Holstege ,  Richard A. Young

发明人： Frank C. P. Holstege ,  Richard A. Young

IPC分类号： C12Q168

CPC分类号： G01N33/5023 ,  C07K14/4702 ,  G01N33/5008 ,  G01N33/5041

摘要： A method of identifying cellular regulatory circuits which employ at least one component of a subcomplex of regulatory proteins within the RNA II polymerase holoenzyme which behaves as a signal processor for gene-specific regulators (at least one component of a eukaryotic transcription initiation apparatus) and of determining the set of components of the apparatus which are responsible for regulation of each gene and the set of genes which are coordinately controlled by each transcription factor.

摘要翻译： 鉴定在RNAII聚合酶全酶中使用调节蛋白亚复合物的至少一种组分的细胞调节电路的方法，其作为基因特异性调节剂（真核转录起始装置的至少一种成分）的信号处理器和 确定负责每个基因的调节的装置的组成和由每个转录因子协调控制的一组基因。

公开(公告)号：US06482614B1

公开(公告)日：2002-11-19

申请号：US09468041

申请日：1999-12-21

申请人： Richard A. Young

发明人： Richard A. Young

IPC分类号： C12P2104

CPC分类号： A61K39/21 ,  A61K39/00 ,  A61K39/0008 ,  A61K39/04 ,  A61K39/12 ,  A61K39/385 ,  A61K39/39 ,  A61K2039/545 ,  A61K2039/55516 ,  A61K2039/6043 ,  A61K2039/70 ,  C07K14/005 ,  C07K14/35 ,  C07K2319/03 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/40 ,  C07K2319/50 ,  C07K2319/735 ,  C07K2319/75 ,  C12N15/62 ,  C12N2740/16222 ,  C12N2740/16234

摘要： The present invention relates to stress proteins and methods of modulating an individual's immune response. In particular, it relates to the use of such stress proteins in immune therapy and prophylaxis, which results in an induction or enhancement of an individual's immune response and as an immunotherapeutic agent which results in a decrease of an individual's immune response to his or her own cells. The present invention also relates to compositions comprising a stress protein joined to another component, such as a fusion protein in which a stress protein is fused to an antigen. Further, the present invention relates to a method of generating antibodies to a substance using a conjugate comprised of a stress protein joined to the substance.

公开(公告)号：US5854055A

公开(公告)日：1998-12-29

申请号：US463942

申请日：1995-06-05

申请人： Barry R. Bloom ,  William R. Jacobs, Jr. ,  Ronald W. Davis ,  Richard A. Young ,  Robert N. Husson

发明人： Barry R. Bloom ,  William R. Jacobs, Jr. ,  Ronald W. Davis ,  Richard A. Young ,  Robert N. Husson

IPC分类号： G01N33/53 ,  A61K39/00 ,  A61K39/04 ,  A61K39/39 ,  A61P31/04 ,  A61P31/06 ,  A61P31/08 ,  A61P31/12 ,  A61P31/16 ,  A61P31/18 ,  A61P31/22 ,  A61P37/04 ,  A61P39/02 ,  C07K14/01 ,  C07K14/35 ,  C12N1/21 ,  C12N15/09 ,  C12N15/74 ,  C12N15/87 ,  C12P21/02 ,  C12R1/19 ,  C12R1/32 ,  C12R1/325 ,  C12R1/33 ,  C12R1/34 ,  G01N33/50 ,  C12N15/64 ,  C12N15/66

CPC分类号： A61K39/04 ,  C07K14/005 ,  C07K14/35 ,  C12N15/74 ,  C12N15/87 ,  C12R1/32 ,  A61K39/00 ,  C12N2795/10022

摘要： Recombinant mycobacterial vaccine vehicles capable of expressing DNA of interest which encodes at least one protein antigen for at least one pathogen against which an immune response is desired and which can be incorporated into the mycobacteria or stably integrated into the mycobacterial genome. The vaccine vehicles are useful for administration to mammalian hosts for purposes of immunization. A recombinant vector which replicates in E. coli but not in mycobacteria is also disclosed. The recombinant vector includes 1) a mycobacterial gene or portions thereof, necessary for recombination with homologous sequences in the genome of mycobacteria transformed with the recombinant plasmid; 2) all or a portion of a gene which encodes a polypeptide or protein whose expression is desired in mycobacteria transformed with the recombinant plasmid; 3) DNA sequences necessary for replication and selection in E. coli; and 4) DNA sequences necessary for selection in mycobacteria (e.g., drug resistance). The present invention also relates to two types of recombinant vectors useful in introducing DNA of interest into mycobacteria, where it is expressed. One type of vector is a recombinant plasmid capable of replicating as a plasmid in E. coli and of lysogenizing a mycobacterial host. The other type of vector is a recombinant plasmid which can be introduced into mycobacteria, where it is stably maintained extrachromosomally.

摘要翻译： 能够表达目的的DNA的重组分枝杆菌疫苗载体，其编码针对至少一种需要免疫应答的至少一种病原体的至少一种蛋白质抗原并且可以并入分枝杆菌中或稳定整合到分枝杆菌基因组中。 疫苗载体用于哺乳动物宿主用于免疫的目的。 还公开了在大肠杆菌中但不在分枝杆菌中复制的重组载体。 重组载体包括1）与用重组质粒转化的分枝杆菌基因组中与同源序列重组所必需的分枝杆菌基因或其部分; 2）编码在用重组质粒转化的分枝杆菌中表达所需的多肽或蛋白质的基因的全部或部分; 3）在大肠杆菌中复制和选择所需的DNA序列; 和4）在分枝杆菌中选择所需的DNA序列（例如耐药性）。 本发明还涉及可用于将感兴趣的DNA引入分枝杆菌的两种类型的重组载体，其中它被表达。 一种载体是能够在大肠杆菌中作为质粒复制并使分解细菌宿主溶解的重组质粒。 另一种载体是可以引入分枝杆菌的重组质粒，其在染色体外稳定地保持。

公开(公告)号：USPP8530P

公开(公告)日：1994-01-04

申请号：US893411

申请日：1992-06-02

申请人： Richard A. Young

发明人： Richard A. Young

摘要： A new variety of Nandina domestica having significant and improved characteristics not known to exist in either the variety "Harbour Dwarf" of which this new plant is a genetic mutant or the general species Nandina domestica. These characteristics include a symmetrical, densely mounding and compact form.

公开(公告)号：US09155724B2

公开(公告)日：2015-10-13

申请号：US13577098

申请日：2011-02-04

申请人： Peter B. Rahl ,  Richard A. Young

发明人： Peter B. Rahl ,  Richard A. Young

IPC分类号： A61K31/435 ,  A61K31/426 ,  C12Q1/18 ,  C12N5/071 ,  A61K31/4025 ,  A61K31/445 ,  A61K31/453 ,  A61K45/06 ,  G01N33/50

CPC分类号： A61K31/4025 ,  A61K31/426 ,  A61K31/445 ,  A61K31/453 ,  A61K45/06 ,  G01N33/5011 ,  G01N2333/4704 ,  A61K2300/00

摘要： In some aspects, the invention provides compositions and methods of use for treating a variety of diseases. In some aspect, the compositions and methods involve combined modulation of transcriptional modulators.

摘要翻译： 在一些方面，本发明提供用于治疗各种疾病的组合物和方法。 在一些方面，组合物和方法涉及转录调节剂的组合调节。