摘要:
A system for dispensing a metered volume of heated water to the brew chamber of a single serve beverage dispenser. The system includes: a storage tank for containing a supply of unheated water, a dispensing tank, and a delivery line communicating with the brew chamber and with the dispensing tank at an intermediate level demarcating the interior of the dispensing tank into upper and lower compartments wherein the volume of the upper compartment is equal to the metered volume. The system also includes a vent valve communicating via a vent line with the upper compartment, and a supply line connecting the storage tank to the lower compartment. An air pump is arranged to deliver pressurized air to the upper compartment. A water pump in the supply line is arranged to deliver water from the storage tank to the lower compartment. A heater for heating water is received in the dispensing tank. Sensing means for generating control signals indicative of the level and temperature of water in the dispensing tank; and a controller enabled by control signals generated by the sensing means and operative in response to a brew signal for operating the air and water pumps in conjunction with the opening and closure of the vent valve to fill the upper compartment with heated water displaced the lower compartment and to discharge the thus displaced heated water from the upper compartment to the brew chamber.
摘要:
A system for dispensing a metered volume of heated water to the brew chamber of a single serve beverage dispenser. The system includes a storage tank for containing a supply of unheated water and a dispensing tank. A delivery line communicates with the brew chamber and with the dispensing tank at an intermediate level demarcating the interior of dispensing tank into upper and lower compartment. The volume of the upper compartment is equal to the metered volume. A vent valve communicates via a vent line with the upper compartment. A supply line connects the storage tank to the lower compartment. An air pump is arranged to deliver pressurized air to the upper compartment. A water pump in the supply line is arranged to deliver water from the storage tank to the lower compartment. A heater for heating water received in the dispensing tank. A sensing mechanism for generating control signals indicative of the level and temperature of water in the dispensing tank. A controller enabled by control signals generated by the sensing means and operative in response to a brew signal for operating the air and water pumps in conjunction with the opening and closure of the vent valve to fill the upper compartment with heated water displaced from the lower compartment and to discharge the thus displaced heated water from the upper compartment to the brew chamber.
摘要:
Methods and prokaryotic expression systems for producing recombinant disulfide bond-containing proteins are described including increasing the level of at least one redox cofactor in the cell to enhance disulfide bond formation are disclosed. Processes of increasing levels of redox cofactors include media supplementation and genetic modification of the host cell. The process can yield increased levels of soluble or active protein, or can assist the proper processing of recombinant proteins in expression systems.
摘要:
An apparatus for inspecting a physical object in a nautical environment includes a radiation source capable of directing a beam of penetrating radiation toward the physical object, and a detector capable of detecting the reaction of the physical object to the penetrating radiation. In addition to detecting the reaction, the detector is capable of delivering an output signal characterizing the physical object. Accordingly, the output signal is based upon the reaction of the physical object to the penetrating radiation.
摘要:
At least two substantially purified tumor necrosis factor (TNF) inhibitors are disclosed which are glycoproteins that are active against TNF. The isolation of 3O kDa and 40 KDa TNF inhibitor from urine is disclosed. The deglycosylated form of the 3O kDa TNF inhibitor and 40 kDa TNF inhibitor are described as being active against TNF. The 40 kDa TNF inhibitor is active against both TNF alpha a TNF beta. The amino acid sequence of the 30 kDa TNF inhibitor and the 40 kDa TNF inhibitor are disclosed. Methods for isolating the TNF inhibitors from human U937 cell medium and producing the proteins by recombinant-DNA methods are also described.
摘要:
The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.
摘要:
The rowing measurement system provides methods and apparatus for improving the performance of a boat by measuring the effect of boat deceleration and providing in-boat feedback to the crew so they can minimize the effect. Specific boat data including speed, distance, stroke rate, acceleration and deceleration can be derived from the deceleration measurements. The data is then processed, displayed, and stored to memory to provide feedback on the boat and crew's performance to the rowers and coaches both in real time and for later analysis. The performance of the boat is measured by an accelerometer and timing device, providing basis for calculating speed, stroke rate, distance, and a deceleration factor without sensors external to the boat. The information on the rowers and the boat is then displayed in real time to the coxswain or head rower to provide immediate feedback on their performance. The data is stored at the CoxDisplay for transmission to the coach or communication to a computer for further display, analysis, communication, and charting a team's improvements.
摘要:
This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.
摘要:
The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.