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    BACTERIAL LEADER SEQUENCES FOR INCREASED EXPRESSION
    3.
    发明申请
    BACTERIAL LEADER SEQUENCES FOR INCREASED EXPRESSION 有权
    细菌引导序列增加表达

    公开(公告)号：US20080193974A1

    公开(公告)日：2008-08-14

    申请号：US12022789

    申请日：2008-01-30

    申请人： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

    发明人： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

    IPC分类号： C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12N1/20 ,  C07K14/00 ,  C12N9/90

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/036

    摘要： Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

    摘要翻译： 提供用于改善宿主细胞中感兴趣的蛋白质或多肽的表达和/或分泌的组合物和方法。 提供了包含细菌分泌信号肽的编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达感兴趣的蛋白质或多肽。 本发明的组合物可用于增加正确加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加来自宿主细胞的适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示氨基酸序列的核苷酸序列的分离的核酸分子。 ，以及SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。

    Bacterial leader sequences for increased expression
    4.
    发明授权
    Bacterial leader sequences for increased expression 有权
    用于增加表达的细菌前导序列

    公开(公告)号：US07618799B2

    公开(公告)日：2009-11-17

    申请号：US12022789

    申请日：2008-01-30

    申请人： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

    发明人： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

    IPC分类号： C12N9/00 ,  C12N15/00 ,  C12N1/04

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/036

    摘要： Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

    摘要翻译： 提供用于改善宿主细胞中感兴趣的蛋白质或多肽的表达和/或分泌的组合物和方法。 提供了包含细菌分泌信号肽的编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达感兴趣的蛋白质或多肽。 本发明的组合物可用于增加正确加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加来自宿主细胞的适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示氨基酸序列的核苷酸序列的分离的核酸分子。 ，以及SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。

    BACTERIAL LEADER SEQUENCES FOR INCREASED EXPRESSION
    5.
    发明申请
    BACTERIAL LEADER SEQUENCES FOR INCREASED EXPRESSION 有权
    细菌引导序列增加表达

    公开(公告)号：US20100048864A1

    公开(公告)日：2010-02-25

    申请号：US12604061

    申请日：2009-10-22

    申请人： Russell J. Coleman ,  Diane Retallack ,  Charles D. Hershberger ,  Stacey Lee

    发明人： Russell J. Coleman ,  Diane Retallack ,  Charles D. Hershberger ,  Stacey Lee

    IPC分类号： C07K14/21 ,  C12P21/06 ,  C12N1/21 ,  C12N15/63 ,  C07H21/04

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/036

    摘要： Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

    摘要翻译： 提供用于改善宿主细胞中感兴趣的多肽的表达和/或分泌的组合物和方法。 提供了包括细菌分泌信号肽的编码序列的组合物。 本发明的组合物可用于增加适当加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 本发明提供了分离的核酸分子，包括编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示的氨基酸序列的核苷酸序列， SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。

    Method and apparatus for migrating file locks from one server to another
    7.
    发明授权
    Method and apparatus for migrating file locks from one server to another 有权
    将文件锁从一个服务器迁移到另一个服务器的方法和装置

    公开(公告)号：US07143097B1

    公开(公告)日：2006-11-28

    申请号：US10632233

    申请日：2003-07-30

    申请人： David F. Olker ,  Stacey Lee

    发明人： David F. Olker ,  Stacey Lee

    IPC分类号： G06F17/30

    CPC分类号： G06F17/30171

    摘要： Using migratory network file system topologies, client file locks are managed by a file lock manager on a primary server. A network file system tracks all client file locks on the primary server. New file locks are identified and retained to support future migration of network file system service to an adoptive server. When a migration of network file system services is needed, the retained file locks are conveyed to the adoptive server. The adoptive server is forced to recognize the conveyed file locks.

    摘要翻译： 使用迁移网络文件系统拓扑，客户端文件锁由主服务器上的文件锁管理器管理。 网络文件系统跟踪主服务器上的所有客户端文件锁。 识别和保留新的文件锁，以支持将网络文件系统服务迁移到收养服务器。 当需要迁移网络文件系统服务时，保留的文件锁将传送给收养服务器。 收养服务器被迫识别传送的文件锁。

    HAND SIZING TOOL
    9.
    发明申请
    HAND SIZING TOOL 有权
    手工工具

    公开(公告)号：US20120084990A1

    公开(公告)日：2012-04-12

    申请号：US12900907

    申请日：2010-10-08

    申请人： Francis J. Masley ,  Ara Atkinson-Skinner ,  Stacey Lee

    发明人： Francis J. Masley ,  Ara Atkinson-Skinner ,  Stacey Lee

    IPC分类号： G01B1/00

    CPC分类号： G01B5/02 ,  A61B5/1072 ,  A61B5/6825

    摘要： A hand sizing tool includes a base platform and a secondary platform affixed to and raised above the base platform. The platforms include a palm heel indicia on the base platform and an index finger tip indicia on the secondary platform. Each of an index finger guide, an index finger measurement slider, and a palm width measurement slider extends out of the secondary platform, these elements being adapted for measuring the hand size.

    摘要翻译： 手动定型工具包括基座平台和固定在基座平台之上并在其上升起的辅助平台。 这些平台包括在基座平台上的棕榈足迹标记和次级平台上的食指尖端标记。 每个食指引导件，食指测量滑块和手掌宽度测量滑块延伸出辅助平台，这些元件适于测量手的尺寸。

    Hand sizing tool
    10.
    发明授权
    Hand sizing tool 有权
    手上胶工具

    公开(公告)号：US08176647B2

    公开(公告)日：2012-05-15

    申请号：US12900907

    申请日：2010-10-08

    申请人： Francis J. Masley ,  Stacey Lee ,  Ara Atkinson-Skinner

    发明人： Francis J. Masley ,  Stacey Lee ,  Ara Atkinson-Skinner

    IPC分类号： G01B5/02 ,  A41H1/02 ,  A61B5/107

    CPC分类号： G01B5/02 ,  A61B5/1072 ,  A61B5/6825

    摘要： A hand sizing tool includes a base platform and a secondary platform affixed to and raised above the base platform. The platforms include a palm heel indicia on the base platform and an index finger tip indicia on the secondary platform. Each of an index finger guide, an index finger measurement slider, and a palm width measurement slider extends out of the secondary platform, these elements being adapted for measuring the hand size.

    摘要翻译： 手动定型工具包括基座平台和固定在基座平台之上并在其上升起的辅助平台。 这些平台包括在基座平台上的棕榈足迹标记和次级平台上的食指尖端标记。 每个食指引导件，食指测量滑块和手掌宽度测量滑块延伸出辅助平台，这些元件适于测量手的尺寸。