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公开(公告)号：US20090162911A1
公开(公告)日：2009-06-25
申请号：US12330531
申请日：2008-12-09
申请人： Robert A. Larossa , Dana R. Smulski
发明人： Robert A. Larossa , Dana R. Smulski
CPC分类号： C12P7/16 , C07K14/245 , C12N1/32 , C12N9/1235 , C12P7/26 , Y02E50/10
摘要： Using screening of transposon random insertion mutants, genes involved in a complex that is a three-component proton motive force-dependent multidrug efflux system were found to be involved in E. coli cell response to butanol. Reduced production of the AcrA and/or AcrB proteins of the complex confers increased butanol tolerance. E. coli strains with reduced AcrA or AcrB production and having a butanol or 2-butanone biosynthetic pathway are useful for production of butanol or 2-butanone.
摘要翻译： 使用转座子随机插入突变体的筛选，发现涉及三组分质子动力依赖多药物流出系统的复合物的基因涉及对丁醇的大肠杆菌细胞应答。 复合物的AcrA和/或AcrB蛋白质的减少产生赋予增加的丁醇耐受性。 具有降低的AcrA或AcrB生产并具有丁醇或2-丁酮生物合成途径的大肠杆菌菌株可用于生产丁醇或2-丁酮。
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2.发明授权Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway 有权具有增加的丁醇耐受性的酵母涉及高渗透压/甘油反应途径公开(公告)号：US08455225B2
公开(公告)日：2013-06-04
申请号：US12643040
申请日：2009-12-21
摘要： Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used for improved butanol production.
摘要翻译： 通过增加高渗透压/甘油反应途径的活性，可以增加对酵母中丁醇的耐受性。 具有增加的PBS2p表达的酵母，高渗透压/甘油反应途径的MAPK模块的丝裂原活化蛋白激酶激酶可用于改善丁醇生产。
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3.发明申请YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING HIGH OSMOLARITY/GLYCEROL RESPONSE PATHWAY 有权YEAST具有增加的高脂肪/甘油反应途径的丁醇耐量公开(公告)号：US20100167365A1
公开(公告)日：2010-07-01
申请号：US12643040
申请日：2009-12-21
摘要： Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used for improved butanol production.
摘要翻译： 通过增加高渗透压/甘油反应途径的活性，可以增加对酵母中丁醇的耐受性。 具有增加的PBS2p表达的酵母，高渗透压/甘油反应途径的MAPK模块的丝裂原活化蛋白激酶激酶可用于改善丁醇生产。
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公开(公告)号：US20090203139A1
公开(公告)日：2009-08-13
申请号：US12330530
申请日：2008-12-09
摘要： Using screening of transposon random insertion mutants, genes involved in accumulation of (p)ppGpp were found to be involved in bacterial cell response to butanol. Reduced production of proteins with enzymatic activity for (p)ppGpp biosynthesis confers increased butanol tolerance. Bacterial strains with reduced (p)ppGpp accumulation and having a butanol or 2-butanone biosynthetic pathway are useful for production of butanol or 2-butanone.
摘要翻译： 使用转座子随机插入突变体的筛选，发现参与（p）ppGpp的积累的基因参与对丁醇的细菌细胞反应。 用于（p）ppGpp生物合成的酶活性降低的蛋白质产生赋予丁醇耐受性提高。 具有降低（p）ppGpp积累并具有丁醇或2-丁酮生物合成途径的细菌菌株可用于生产丁醇或2-丁酮。
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5.发明授权Yeast with increased butanol tolerance involving cell wall integrity pathway 有权酵母具有提高的丁醇耐受性，涉及细胞壁完整性途径公开(公告)号：US08795992B2
公开(公告)日：2014-08-05
申请号：US12643030
申请日：2009-12-21
CPC分类号： C12P7/16 , C12N9/1205 , C12N15/81 , Y02E50/10
摘要： Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the cell wall integrity pathway. Yeast with increased expression of SLT2p, a mitogen activated protein kinase of the MAPK module of the cell wall integrity pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.
摘要翻译： 通过提高细胞壁完整性途径的活性已经增加了对酵母中丁醇的耐受性。 具有增加的SLT2p表达的酵母，细胞壁完整性途径的MAPK模块的丝裂原活化蛋白激酶具有增加的对异丁醇的耐受性。 这些酵母可用于改善丁醇生产。
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公开(公告)号：US08372612B2
公开(公告)日：2013-02-12
申请号：US12330530
申请日：2008-12-09
摘要： Using screening of transposon random insertion mutants, genes involved in accumulation of (p)ppGpp were found to be involved in bacterial cell response to butanol. Reduced production of proteins with enzymatic activity for (p)ppGpp biosynthesis confers increased butanol tolerance. Bacterial strains with reduced (p)ppGpp accumulation and having a butanol or 2-butanone biosynthetic pathway are useful for production of butanol or 2-butanone.
摘要翻译： 使用转座子随机插入突变体的筛选，发现参与（p）ppGpp积累的基因参与对丁醇的细菌细胞反应。 用于（p）ppGpp生物合成的酶活性降低的蛋白质产生赋予丁醇耐受性提高。 具有降低（p）ppGpp积累并具有丁醇或2-丁酮生物合成途径的细菌菌株可用于生产丁醇或2-丁酮。
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7.发明授权Strain comprising increased expression of a CFA coding region for butanol production 有权包含增加用于丁醇生产的CFA编码区的表达的菌株公开(公告)号：US08518678B2
公开(公告)日：2013-08-27
申请号：US12330534
申请日：2008-12-09
CPC分类号： C12P7/16 , C12N9/1007 , Y02E50/10
摘要： Screening of fatty acid fed bacteria which are not natural butanol producers identified increased membrane cyclopropane fatty acid as providing improved butanol tolerance. Increasing expression of cyclopropane fatty acid synthase in the presence of the enzyme substrate that is either endogenous to the cell or fed to the cell, increased butanol tolerance. Bacterial strains with increased cyclopropane fatty acid synthase and having a butanol biosynthetic pathway are useful for production of butanol.
摘要翻译： 筛选不是天然丁醇生产者的脂肪酸饲养细菌增加膜环丙烷脂肪酸提供改善的丁醇耐受性。 在酶底物存在下增加环丙烷脂肪酸合酶的表达，该酶底物是细胞内源的或进入细胞，提高了丁醇耐受性。 具有增加的环丙烷脂肪酸合成酶并具有丁醇生物合成途径的细菌菌株可用于生产丁醇。
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8.发明申请YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING FILAMENTOUS GROWTH RESPONSE 有权YEAST与增加勃艮第生长反应的勃艮第耐受性公开(公告)号：US20100167363A1
公开(公告)日：2010-07-01
申请号：US12643019
申请日：2009-12-21
摘要： Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the filamentous growth response. Yeast with increased expression of MSS11p, a transcriptional activator of the filamentous growth response pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.
摘要翻译： 通过增加丝状生长反应的活性已经增加了对酵母中丁醇的耐受性。 具有增加的丝氨酸生长反应途径的转录激活因子MSS11p的表达的酵母具有增加的对异丁醇的耐受性。 这些酵母可用于改善丁醇生产。
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公开(公告)号：US06607885B1
公开(公告)日：2003-08-19
申请号：US09686383
申请日：2000-10-11
申请人： Robert A. Larossa , Yan Wei
发明人： Robert A. Larossa , Yan Wei
IPC分类号： C12Q168
CPC分类号： C12Q1/689 , C12Q1/6837 , C12Q2600/158
摘要： The global effect on genes under different environmental conditions can be determined by a comprehensive gene expression profile. The present invention provides a method to monitor the changes in comprehensive cellular gene expression levels at single length resolution by using a high-density microarray prepared with a comprehensive collection of ORFs of a genome. Under different environmental conditions, directly and indirectly affected genes can be detected as the gene expression levels are induced or repressed in comparison to the control.
摘要翻译： 在不同环境条件下对基因的全球影响可以通过综合基因表达谱来确定。 本发明提供了一种通过使用用基因组的ORF的全面收集制备的高密度微阵列来监测单长分辨率的综合细胞基因表达水平的变化的方法。 在不同的环境条件下，与对照相比，可以检测到直接和间接影响的基因，因为基因表达水平被诱导或抑制。
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公开(公告)号：US20090203097A1
公开(公告)日：2009-08-13
申请号：US12330534
申请日：2008-12-09
CPC分类号： C12P7/16 , C12N9/1007 , Y02E50/10
摘要： Screening of fatty acid fed bacteria which are not natural butanol producers identified increased membrane cyclopropane fatty acid as providing improved butanol tolerance. Increasing expression of cyclopropane fatty acid synthase in the presence of the enzyme substrate that is either endogenous to the cell or fed to the cell, increased butanol tolerance. Bacterial strains with increased cyclopropane fatty acid synthase and having a butanol biosynthetic pathway are useful for production of butanol.
摘要翻译： 筛选不是天然丁醇生产者的脂肪酸饲养细菌增加膜环丙烷脂肪酸提供改善的丁醇耐受性。 在酶底物存在下增加环丙烷脂肪酸合酶的表达，该酶底物是细胞内源的或进入细胞，提高了丁醇耐受性。 具有增加的环丙烷脂肪酸合成酶并具有丁醇生物合成途径的细菌菌株可用于生产丁醇。
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