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1.发明授权Yeast with increased butanol tolerance involving a multidrug efflux pump gene 有权具有增加的丁醇耐受性的酵母涉及多药外排泵基因公开(公告)号：US08614085B2
公开(公告)日：2013-12-24
申请号：US12711308
申请日：2010-02-24
申请人： Tina K. Van Dyk
发明人： Tina K. Van Dyk
摘要： Increasing tolerance to butanol in yeast has been accomplished by decreasing activity of Pdr5p encoded by an endogenous PDR5 gene. A deletion mutation of the PDR5 gene led to improved growth yield in the presence of butanol. Yeast cells with reduced Pdr5p activity, or other multidrug resistance ATP-binding cassette transporter protein activity encoded by CDR1 or BFR1, and a butanol biosynthetic pathway may be used for improved butanol production.
摘要翻译： 通过降低由内源性PDR5基因编码的Pdr5p的活性，可以增加对酵母中丁醇的耐受性。 PDR5基因的缺失突变导致丁醇存在下生长产量提高。 可以使用具有降低的Pdr5p活性的酵母细胞或由CDR1或BFR1编码的其他多药耐药性ATP结合盒转运蛋白活性和丁醇生物合成途径来改善丁醇生产。
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2.发明授权公开(公告)号：US08569036B2
公开(公告)日：2013-10-29
申请号：US11485575
申请日：2006-07-12
申请人： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Tina K. Van Dyk , Robert J. Trotman
发明人： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Tina K. Van Dyk , Robert J. Trotman
摘要： TAL cell biocatalyst was immobilized in alginate cross-linked beads using low concentrations of glutaraldehyde. The biocatalyst beads have highly stable TAL activity and mechanical strength such that they withstand prolonged recycling in production of pHCA.
摘要翻译： 使用低浓度戊二醛将TAL细胞生物催化剂固定在藻酸盐交联珠粒中。 生物催化剂珠具有高度稳定的TAL活性和机械强度，使得它们在pHCA的生产中经受长时间的再循环。
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公开(公告)号：US08222000B2
公开(公告)日：2012-07-17
申请号：US12960634
申请日：2010-12-06
申请人： Tina K. Van Dyk
发明人： Tina K. Van Dyk
IPC分类号： C12P21/06
摘要： Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct: a nucleotide sequence from Bacillus licheniformis ATCC® 14580 encoding a polypeptide having sucrose transporter activity and a nucleotide sequence from Bacillus licheniformis ATCC® 14580 encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
摘要翻译： 描述了能够代谢蔗糖的重组细菌。 重组细菌在其基因组或至少一种重组构建体中包含：编码具有蔗糖转运蛋白活性的多肽的地衣芽孢杆菌ATCC 14580的核苷酸序列和编码具有蔗糖水解酶活性的多肽的地衣芽孢杆菌ATCC 14580的核苷酸序列。 这些核苷酸序列各自可操作地连接到相同或不同的启动子。 还描述了能够代谢蔗糖以产生甘油和/或甘油衍生产物如1,3-丙二醇和3-羟基丙酸的重组细菌。
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公开(公告)号：US20130230893A1
公开(公告)日：2013-09-05
申请号：US13412193
申请日：2012-03-05
CPC分类号： C12P7/20 , C07K14/195 , C07K14/24 , C07K14/245 , C07K14/33 , C07K14/335 , C12N9/1051 , C12N9/1205 , C12N9/2402 , C12N9/2431 , C12P7/18 , C12P7/42 , C12Y204/01007 , C12Y207/01001 , C12Y207/01003 , C12Y207/01004 , C12Y302/01026
摘要： Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
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公开(公告)号：US08129170B1
公开(公告)日：2012-03-06
申请号：US12960646
申请日：2010-12-06
申请人： Tina K. Van Dyk
发明人： Tina K. Van Dyk
CPC分类号： C12P7/18 , C12N9/1205 , C12N9/2402 , C12N9/2405 , C12N9/2431 , C12P7/20 , C12P7/42 , C12Y207/01004 , C12Y302/01048
摘要： Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct: a nucleotide sequence from Pseudomonas fluorescens Pf5 (ATCC® BAA-477) encoding a polypeptide having sucrose transporter activity and a nucleotide sequence from Pseudomonas fluorescens Pf5 (ATCC® BAA-477) encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
摘要翻译： 描述了能够代谢蔗糖的重组细菌。 重组细菌在其基因组或至少一种重组构建体中包含：编码具有蔗糖转运蛋白活性的多肽的荧光假单胞菌Pf5（ATCCBAA-477）的核苷酸序列和来自荧光假单胞菌Pf5（BABA-477）的核苷酸序列 ）编码具有蔗糖水解酶活性的多肽。 这些核苷酸序列各自可操作地连接到相同或不同的启动子。 还描述了能够代谢蔗糖以产生甘油和/或甘油衍生产物如1,3-丙二醇和3-羟基丙酸的重组细菌。
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公开(公告)号：US20110195505A1
公开(公告)日：2011-08-11
申请号：US12899760
申请日：2010-10-07
申请人： LORI JEAN EULER , DENNIS FLINT , BRIAN JAMES PAUL , TINA K. VAN DYK , RICK W. YE
发明人： LORI JEAN EULER , DENNIS FLINT , BRIAN JAMES PAUL , TINA K. VAN DYK , RICK W. YE
CPC分类号： C12P7/16 , C07K14/335 , C12N1/36 , Y02E50/10
摘要： Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the saturated fatty acids content in bacterial cell membrane was increased. Methods for increasing the concentration of saturated fatty acids in the membranes of bacteria that are not natural butanol produces are described whereby tolerance of the bacterial cell to butanol is increased. Saturated fatty acids concentration in the bacterial cell membrane increased upon exogenously feeding saturated fatty acids to cells. Bacterial strains useful for production of butanol are described herein having modified unsaturated fatty acid biosynthetic pathway.
摘要翻译： 当细菌细胞膜中的饱和脂肪酸含量增加时，发现不是天然丁醇生产者的细菌对丁醇具有增加的耐受性。 描述了增加非天然丁醇产生的细菌膜中饱和脂肪酸浓度的方法，其中细菌细胞对丁醇的耐受性增加。 饱和脂肪酸在细胞细胞膜中的浓度在将饱和脂肪酸外源供给细胞后增加。 本文描述了可用于生产丁醇的细菌菌株，其具有改性的不饱和脂肪酸生物合成途径。
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公开(公告)号：US20080118958A1
公开(公告)日：2008-05-22
申请号：US11448331
申请日：2006-06-07
CPC分类号： C12P13/225
摘要： An enteric bacterial strain was engineered to over-produce L-tyrosine using a one-step method. The pheA-tyrA chromosomal region of the bacterial genome was replaced with an engineered chromosomal segment, resulting in inactivation of the pheA coding region and strong expression of the tyrA coding region, resulting in high levels of L-tyrosine production.
摘要翻译： 使用一步法将肠道细菌菌株设计为过度产生L-酪氨酸。 细菌基因组的pheA-tyrA染色体区被工程染色体片段替代，导致pheA编码区的失活和tyrA编码区的强表达，导致高水平的L-酪氨酸产生。
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公开(公告)号：US20080102499A1
公开(公告)日：2008-05-01
申请号：US11588994
申请日：2006-10-27
CPC分类号： C12P13/225 , C12N9/001
摘要： Tyrosine production in a tyrosine over-producing enteric bacterial strain was enhanced by expression of a tyrosine insensitive prephenate dehydrogenase. The prephenate dehydrogenase expressed was the cyclohexadienyl dehydrogenase encoded by the Zymomonas mobilis tyrc gene.
摘要翻译： 通过酪氨酸不敏感的前苯甲酸脱氢酶的表达增强了酪氨酸过度生产的肠道细菌菌株中酪氨酸的产生。 表达的前苯甲酸脱氢酶是由运动发酵单胞菌tyrc基因编码的环己二烯基脱氢酶。
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9.发明授权Methods for increasing the resistance of a host cell to aromatic carboxylic acids 失效增加宿主细胞对芳香族羧酸的抗性的方法公开(公告)号：US07279321B2
公开(公告)日：2007-10-09
申请号：US10759898
申请日：2004-01-16
CPC分类号： C12P7/40 , C07K14/245
摘要： The invention provides methods of increasing the production of aromatic carboxylic acids from a host cell via manipulation of the yhcRQP operon encoding a family of efflux proteins. Up-regulation of all or a sub-set of the genes in the yhcRQP were additionally found to enhance tolerance to aromatic carboxylic acids toxicity.
摘要翻译： 本发明提供了通过操纵编码一系列外排蛋白的yhcRQP操纵子来增加宿主细胞产生芳族羧酸的方法。 另外发现yhcRQP中全部或一组亚基的上调增强了对芳香族羧酸毒性的耐受性。
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公开(公告)号：US06716582B2
公开(公告)日：2004-04-06
申请号：US09832419
申请日：2001-04-11
申请人： Gregory E. Gonye , Michael K. Hanafey , Robert A. LaRossa , J. Antoni Rafalski , Tina K. Van Dyk
发明人： Gregory E. Gonye , Michael K. Hanafey , Robert A. LaRossa , J. Antoni Rafalski , Tina K. Van Dyk
IPC分类号： C12Q168
CPC分类号： C12Q1/6897
摘要： The present invention relates to the generation and use of a cellular array or a cellular array in combination with other genome-registered arrays (an array of arrays) for the determination of gene function and/or perturbation mode of action. Each cellular array consists of a number of microbial strains. Each strain comprises one reporter gene fusion made up of a gene or gene fragment operably linked to a reporter gene. Each gene or gene fragment has been “registered” or mapped to a specific location in the genome of the organism. The genome-registered collection of the invention may be used to determine alterations in gene expression under a variety of conditions. Such collections are amenable to rapid assay and may be used to confirm, correct or augment data generated from DNA micro array technology.
摘要翻译： 本发明涉及与用于确定基因功能和/或扰动作用模式的其他基因组登记阵列（阵列阵列）组合的细胞阵列或细胞阵列的产生和使用。 每个细胞阵列由许多微生物菌株组成。 每个菌株包含由与报告基因可操作地连接的基因或基因片段组成的一个报告基因融合体。 每个基因或基因片段已被“注册”或映射到生物体的基因组中的特定位置。 可以使用本发明的基因组登记的集合来确定在各种条件下基因表达的改变。 这样的收集可适用于快速测定，并且可用于确认，校正或增加从DNA微阵列技术产生的数据。
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