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公开(公告)号：US07332333B2
公开(公告)日：2008-02-19
申请号：US11018895
申请日：2004-12-21
IPC分类号： C12N15/867 , C12N5/10
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N2510/02 , C12N2730/10122 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
摘要翻译： 本发明涉及宿主细胞中蛋白质的制备，更具体地涉及含有整合载体的多个整合拷贝的宿主细胞。 用于本发明的合适的整合载体包括逆转录病毒载体，慢病毒载体，转座子载体和腺相关病毒载体。 提供了其中通过以高倍数感染使用整合载体制备宿主细胞的方法。 宿主细胞可用于生产药物蛋白质，用于筛选测定的蛋白质变体，以及直接用于高通量筛选。
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公开(公告)号：US06852510B2
公开(公告)日：2005-02-08
申请号：US09897511
申请日：2001-06-29
IPC分类号： C07K14/02 , C07K16/12 , C07K16/30 , C12N7/04 , C12N15/82 , C12N15/867 , C12P21/02 , C12N15/09 , C12N5/10 , C12N15/63
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N2510/02 , C12N2730/10122 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
摘要： The present invention relates to the production of proteins in host cells, and, more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
摘要翻译： 本发明涉及在宿主细胞中产生蛋白质，更具体地涉及含有多个整合载体的集成拷贝的宿主细胞。 用于本发明的合适的整合载体包括逆转录病毒载体，慢病毒载体，转座子载体和腺相关病毒载体。 提供了其中通过以高倍数感染使用整合载体制备宿主细胞的方法。 宿主细胞可用于生产药物蛋白质，用于筛选测定的蛋白质变体，以及直接用于高通量筛选。
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公开(公告)号：US20080286779A1
公开(公告)日：2008-11-20
申请号：US11928464
申请日：2007-10-30
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N2510/02 , C12N2730/10122 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
摘要翻译： 本发明涉及宿主细胞中蛋白质的制备，更具体地涉及含有整合载体的多个整合拷贝的宿主细胞。 用于本发明的合适的整合载体包括逆转录病毒载体，慢病毒载体，转座子载体和腺相关病毒载体。 提供了其中通过以高倍数感染使用整合载体制备宿主细胞的方法。 宿主细胞可用于生产药物蛋白质，用于筛选测定的蛋白质变体，以及直接用于高通量筛选。
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