FACILITATED PROCESS FOR PURIFICATION OF PROTEINS
    2.
    发明申请
    FACILITATED PROCESS FOR PURIFICATION OF PROTEINS 有权
    用于纯化蛋白质的方法

    公开(公告)号:US20110250673A1

    公开(公告)日:2011-10-13

    申请号:US13070118

    申请日:2011-03-23

    IPC分类号: C12N9/18

    CPC分类号: C12N9/18 C12Y301/01072

    摘要: A two-stage heat treatment process is provided to improve the processability of recombinant microbial biomass comprising an enzyme having perhydrolytic activity. More specifically, a process is provided to treat the recombinant microbial biomass comprising a Thermotoga sp. acetyl xylan esterase having perhydrolytic activity such that microfiltration may be used to partially-purify and/or concentrate protein preparations. The acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

    摘要翻译: 提供两阶段热处理方法以改善包含具有过水解活性的酶的重组微生物生物质的加工性能。 更具体地,提供了一种处理包含Thermotoga sp。的重组微生物生物质的方法。 具有过水解活性的乙酰木聚糖酯酶,使得微量过滤可用于部分纯化和/或浓缩蛋白质制剂。 乙酰木聚糖酯酶可用于制备适用于各种应用的过氧羧酸,例如清洁,消毒,消毒,漂白,木浆加工和纸浆加工应用。