-
1.发明授权Pseudomonas putida amidase polypeptide useful for the production of chiral amides and acids 失效用于生产手性酰胺和酸的恶臭假单胞菌多糖酶多糖公开(公告)号：US06251650B1
公开(公告)日：2001-06-26
申请号：US09687594
申请日：2000-10-13
IPC分类号： C12N978
CPC分类号： C12P13/02 , C12N9/78 , C12N9/80 , C12P7/40 , C12P17/00 , C12P41/006 , Y10S435/877
摘要： The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
摘要翻译： 本发明提供从恶臭假单胞菌（Pseudomonas putida）分离的腈水合酶核酸片段，其编码能够将某些外消旋腈水解成相应的R-或S-酰胺的腈水合酶活性。 还提供了能够主动表达所述腈水合酶活性的转化微生物。 另外，本发明提供了一种携带腈水合酶基因的转化体，其与酰胺酶基因结合，两者都可以分别共同表达产生活性腈水合酶和酰胺酶。 还提供了生产这种对映体材料的方法。
-
2.发明授权Polypeptides and polypeptide subunits of a stereospecific nitrile hydratase enzyme 失效立体特异性腈水合酶的多肽和多肽亚基
公开(公告)号：US6133421A
公开(公告)日：2000-10-17
申请号：US103434
申请日：1998-06-24
IPC分类号： C12N15/09 , C07K14/21 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/78 , C12N9/80 , C12N15/00 , C12N15/55 , C12P7/40 , C12P13/02 , C12P17/00 , C12P41/00 , C12R1/19 , C12R1/40 , C12R1/84 , C07H21/04 , C12N1/00 , C12N1/20 , C12N9/88
CPC分类号： C12P13/02 , C12N9/78 , C12N9/80 , C12P17/00 , C12P41/006 , C12P7/40 , Y10S435/877
摘要： 350 The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
摘要翻译： 350本发明提供从恶臭假单胞菌分离的腈水合酶核酸片段，其编码能够催化某些外消旋腈水解成相应的R-或S-酰胺的腈水合酶活性。 还提供了能够主动表达所述腈水合酶活性的转化微生物。 另外，本发明提供了一种携带腈水合酶基因的转化体，其与酰胺酶基因结合，两者都可以分别共同表达产生活性腈水合酶和酰胺酶。 还提供了生产这种对映体材料的方法。
-
3.发明授权Method for using hydratase or a hydratase-amidase fusion for stereospecifically bioconverting certain racemic nitriles to the corresponding enatiomeric R--or S-amide or s-carboxylic acid 失效将水合酶或水合酶 - 酰胺酶融合物用于立体特异性生物转化某些外消旋腈到相应的对位R或S-酰胺或s-羧酸的方法
公开(公告)号：US5888785A
公开(公告)日：1999-03-30
申请号：US103411
申请日：1998-06-24
IPC分类号： C12N15/09 , C07K14/21 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/78 , C12N9/80 , C12N15/00 , C12N15/55 , C12P7/40 , C12P13/02 , C12P17/00 , C12P41/00 , C12R1/19 , C12R1/40 , C12R1/84 , C12N9/88
CPC分类号： C12P13/02 , C12N9/78 , C12N9/80 , C12P17/00 , C12P41/006 , C12P7/40 , Y10S435/877
摘要： The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitrites to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
摘要翻译： 本发明提供从恶臭假单胞菌（Pseudomonas putida）分离的腈水合酶核酸片段，其编码能够将某些外消旋亚硝酸盐水解成相应的R-或S-酰胺的腈水合酶活性。 还提供了能够主动表达所述腈水合酶活性的转化微生物。 另外，本发明提供了一种携带腈水合酶基因的转化体，其与酰胺酶基因结合，两者都可以分别共同表达产生活性腈水合酶和酰胺酶。 还提供了生产这种对映体材料的方法。
-
4.发明授权Nucleic acid fragments encoding stereospecific nitrile hydratase and amidase enzymes and recombinant organisms expressing those enzymes useful for the production of chiral amides and acids 失效编码立体特异性腈水合酶和酰胺酶的核酸片段和表达可用于生产手性酰胺和酸的那些酶的重组生物
公开(公告)号：US5811286A
公开(公告)日：1998-09-22
申请号：US726136
申请日：1996-10-04
IPC分类号： C12N15/09 , C07K14/21 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/78 , C12N9/80 , C12N15/00 , C12N15/55 , C12P7/40 , C12P13/02 , C12P17/00 , C12P41/00 , C12R1/19 , C12R1/40 , C12R1/84 , C12N1/20 , C07H21/04 , C12N9/88
CPC分类号： C12P13/02 , C12N9/78 , C12N9/80 , C12P17/00 , C12P41/006 , C12P7/40 , Y10S435/877
摘要： The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
摘要翻译： 本发明提供从恶臭假单胞菌（Pseudomonas putida）分离的腈水合酶核酸片段，其编码能够将某些外消旋腈水解成相应的R-或S-酰胺的腈水合酶活性。 还提供了能够主动表达所述腈水合酶活性的转化微生物。 另外，本发明提供了一种携带腈水合酶基因的转化体，其与酰胺酶基因结合，两者都可以分别共同表达产生活性腈水合酶和酰胺酶。 还提供了生产这种对映体材料的方法。
-
-
-
搜索结果
4 条记录 (耗时 0.02 秒)
