公开(公告)号：US20070228049A1

公开(公告)日：2007-10-04

申请号：US11759355

申请日：2007-06-07

申请人： Robert Nordmeyer ,  Gyorgy Snell ,  Earl Cornell ,  William Kolbe ,  Derek Yegian ,  Thomas Earnest ,  Joseph Jaklevic ,  Carl Cork ,  Bernard Santarsiero ,  Raymond Stevens

发明人： Robert Nordmeyer ,  Gyorgy Snell ,  Earl Cornell ,  William Kolbe ,  Derek Yegian ,  Thomas Earnest ,  Joseph Jaklevic ,  Carl Cork ,  Bernard Santarsiero ,  Raymond Stevens

IPC分类号： F17C3/00 ,  F25D17/02 ,  G01N23/207

CPC分类号： G01N23/20025 ,  B01L9/065 ,  B01L2200/18 ,  B01L2300/1883 ,  C30B29/58 ,  C30B33/00 ,  C30B35/005 ,  G01N1/42 ,  G01N23/207

摘要： A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

摘要翻译： 用于运输，远程和无人值守安装的方法和装置，以及用于同步加速器产生的x射线衍射分析的蛋白质晶体的视觉对准和监测。 蛋白质样品始终保持在液氮温度：装运期间，安装，安装，对准，数据采集和拆卸之后。 样品必须在空间的某一点处另外稳定地对准至几微米。 远程和自动地准确执行这些任务的能力导致大量蛋白质表征工作的样品通量和可靠性的显着增加。 由于将蛋白质样品放置在每个容纳多个样品的样品盒的运输兼容层叠堆叠中，因此大量样品可以在单个低温运输容器中运输。

公开(公告)号：US20050163280A1

公开(公告)日：2005-07-28

申请号：US11064357

申请日：2005-02-22

申请人： Robert Nordmeyer ,  Gyorgy Snell ,  Earl Cornell ,  William Kolbe ,  Derek Yegian ,  Thomas Earnest ,  Joseph Jaklevic ,  Carl Cork ,  Bernard Santarsiero ,  Raymond Stevens

发明人： Robert Nordmeyer ,  Gyorgy Snell ,  Earl Cornell ,  William Kolbe ,  Derek Yegian ,  Thomas Earnest ,  Joseph Jaklevic ,  Carl Cork ,  Bernard Santarsiero ,  Raymond Stevens

IPC分类号： C30B33/00 ,  C30B35/00 ,  G01N23/20

CPC分类号： G01N23/20025 ,  B01L9/065 ,  B01L2200/18 ,  B01L2300/1883 ,  C30B29/58 ,  C30B33/00 ,  C30B35/005 ,  G01N1/42 ,  G01N23/207

摘要： A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

公开(公告)号：US20070050152A1

公开(公告)日：2007-03-01

申请号：US11510422

申请日：2006-08-24

申请人： Raymond Stevens ,  Peter Kuhn ,  Maneesh Yadav

发明人： Raymond Stevens ,  Peter Kuhn ,  Maneesh Yadav

IPC分类号： G06F19/00

CPC分类号： G01N23/20025 ,  C07K5/0613 ,  C07K2299/00 ,  G01N2223/612

摘要： A method is provided for acquiring X-ray diffraction images of a protein crystal, the method comprising providing an X-ray source and a micro-channel, wherein the micro-channel contains an oil, gas or immiscible liquid and at least one aqueous droplet comprising a crystal of a protein, recording an X-ray diffraction image of the crystal, repositioning the crystal with respect to the X-ray source, and repeating the last two steps immediately above at least once.

摘要翻译： 提供了一种用于获取蛋白质晶体的X射线衍射图像的方法，所述方法包括提供X射线源和微通道，其中所述微通道包含油，气体或不混溶液体和至少一个水滴 包括蛋白质的晶体，记录晶体的X射线衍射图像，相对于X射线源重新定位晶体，并且至少重复上述两个步骤至少一次。

公开(公告)号：US20070048855A1

公开(公告)日：2007-03-01

申请号：US11230374

申请日：2005-09-19

申请人： Alejandra Gamez ,  Lin Wang ,  Woomi Kim ,  Mary Straub ,  Marianne Patch ,  Emil Kakkis ,  Dan Oppenheimer ,  Paul Fitzpatrick ,  Robert Heft ,  Raymond Stevens

发明人： Alejandra Gamez ,  Lin Wang ,  Woomi Kim ,  Mary Straub ,  Marianne Patch ,  Emil Kakkis ,  Dan Oppenheimer ,  Paul Fitzpatrick ,  Robert Heft ,  Raymond Stevens

IPC分类号： C12N9/88 ,  G06F19/00

CPC分类号： C12N9/88 ,  C07K2299/00 ,  Y02A90/26

摘要： The present invention pertains to the use of the protein phenylalanine ammonia-lyase, as well as the biologically-active derivatives of the said protein for preventing or treating diseases associated with a phenylalanine imbalance in a human or animal body. More particularly, the present invention relates to the therapeutic use of the above-cited molecules for preventing or treating a phenylalanine imbalance in vivo. This invention also deals with therapeutic compositions comprising a pharmaceutically active amount of the above-described therapeutic molecules as well as with therapeutic methods using the said therapeutic compositions. Finally, the present invention relates to processes for selecting more therapeutically-effective variants of said protein as well as to the selected variants themselves.

摘要翻译： 本发明涉及蛋白质苯丙氨酸氨裂解酶的用途，以及所述蛋白质的生物活性衍生物用于预防或治疗与人或动物体内苯丙氨酸不平衡有关的疾病。 更具体地说，本发明涉及上述分子用于预防或治疗体内苯丙氨酸失衡的治疗用途。 本发明还涉及包含药物活性量的上述治疗分子以及使用所述治疗组合物的治疗方法的治疗组合物。 最后，本发明涉及用于选择所述蛋白质的更多治疗上有效的变体以及所选择的变体本身的方法。