公开(公告)号：US20070034140A1

公开(公告)日：2007-02-15

申请号：US11463033

申请日：2006-08-08

申请人： Robert Thorne ,  Viatcheslav Berejnov ,  Yevgeniy Kalinin

发明人： Robert Thorne ,  Viatcheslav Berejnov ,  Yevgeniy Kalinin

IPC分类号： C30B7/00 ,  C30B21/02 ,  C30B28/06

CPC分类号： C30B7/00 ,  C30B7/04 ,  C30B29/58

摘要： In one embodiment, a crystallization and screening plate comprises a plurality of cells open at a top and a bottom, a frame that defines the cells in the plate, and at least two films. The first film seals a top of the plate and the second film seals a bottom of the plate. At least one of the films is patterned to strongly pin the contact lines of drops dispensed onto it, fixing their position and shape. The present invention also includes methods and other devices for manual and high-throughput protein crystal growth.

摘要翻译： 在一个实施方案中，结晶和筛选板包括在顶部和底部开放的多个单元，限定板中的单元的框架和至少两个薄膜。 第一个膜密封板的顶部，第二个膜密封板的底部。 至少一个膜被图案化以强力地分配分配到其上的液滴的接触线，固定它们的位置和形状。 本发明还包括用于手动和高通量蛋白质晶体生长的方法和其它装置。

公开(公告)号：US20070242260A1

公开(公告)日：2007-10-18

申请号：US11698277

申请日：2007-01-26

申请人： Robert Thorne ,  Guanhan Chew

发明人： Robert Thorne ,  Guanhan Chew

IPC分类号： G01N1/00

CPC分类号： G01N1/286 ,  B01L3/505 ,  B01L3/5088 ,  B01L2200/147 ,  B01L2300/028

摘要： Microfabricated tools useful for manipulating small, delicate samples are formed from thin plastic films. The films have a small thickness (preferably 5 to 50 micrometers and typically 10 micrometers) and small lateral dimensions (preferably 2 mm or less and typically 0.1 to 1 mm) so that they are reasonably flexible, but are preferably curved by being wrapped around a cylindrical or flat post to give them some rigidity. The softness and thinness of the plastic reduce risk of sample damage during incidental contact with the tool. Its thinness makes it optically and X-ray transparent, so that the samples can be clearly visualized during manipulations and so that the tools can be used to collect X-ray data from samples. As an option, an X-ray sensitive phosphor is incorporated in the film at low concentration. This allows the X-ray beam to be visually located on the mount or tool. The plastic can also be treated to obtain a desired hydrophobicity or hydrophilicity, mechanically embossed or abraded, or coated with films (for example, of polyethylene glycol via pegylation procedures or of PDMS) that promote or inhibit sample adhesion.

摘要翻译： 用于操作小而精细样品的微型工具由薄塑料膜形成。 膜的厚度（优选为5至50微米，典型为10微米）和较小的横向尺寸（优选2mm或更小，通常为0.1至1毫米），使得它们具有相当的柔性，但优选通过围绕 圆柱形或扁平柱，给它们一些刚性。 塑料的柔软性和薄度降低了与工具偶然接触时样品损坏的风险。 其薄度使其光学和X射线透明，使样品在操作过程中可以清晰可视，从而可以使用这些工具从样品采集X射线数据。 作为选择，在低浓度下将X射线敏感性荧光体掺入膜中。 这允许X射线束在视觉上位于安装件或工具上。 也可以处理塑料以获得所需的疏水性或亲水性，机械压花或磨损，或涂覆促进或抑制样品粘附的膜（例如聚乙二醇通过聚乙二醇化程序或PDMS）。

公开(公告)号：US6021310A

公开(公告)日：2000-02-01

申请号：US940881

申请日：1997-09-30

申请人： Robert Thorne

发明人： Robert Thorne

IPC分类号： G08B5/22 ,  H04W4/12 ,  H04W68/00 ,  H04W84/04 ,  H04W88/02 ,  H04Q7/00

CPC分类号： H04W68/00 ,  G08B5/228 ,  H04W4/12 ,  H04W84/042 ,  H04W88/02

摘要： A computerized paging device connected a source of power for transmitting and receiving messages. The computerized paging device includes a modem for transmitting messages to a desired party and receiving message signals transmitted to the device and a microprocessor including a memory unit for storing programming information for the microprocessor is connected to the modem for processing the received message signals and generating a display message signal based upon the processed received message signal. A display screen is connected to the microprocessor for displaying the display message signal. A speaker is connected to the microprocessor for producing an audible representation of the display message signal and a microphone is also connected to the microprocessor for receiving an audible signal, wherein the audible signal is transmitted to the microprocessor for conversion into a digital signal and transmission to the desired party by the modem. A mouse can be connected to the microprocessor for scrolling through the message displayed on the display screen.

摘要翻译： 连接有用于发送和接收消息的电源的计算机化寻呼装置。 计算机化寻呼装置包括用于向期望方发送消息并接收发送到设备的消息信号的调制解调器，并且包括用于存储用于微处理器的节目信息的存储器单元的微处理器连接到调制解调器以处理接收到的消息信号并产生 基于处理的接收到的消息信号显示消息信号。 显示屏连接到微处理器以显示显示消息信号。 扬声器连接到微处理器以产生显示消息信号的可听见的表示，并且麦克风也连接到微处理器以接收可听信号，其中，可听信号被发送到微处理器以转换成数字信号并传输到 调制解调器所需的一方。 鼠标可以连接到微处理器以滚动显示在显示屏幕上的消息。

公开(公告)号：USD697274S1

公开(公告)日：2014-01-07

申请号：US29444015

申请日：2013-01-25

申请人： Robert Thorne

设计人： Robert Thorne

公开(公告)号：US20060086315A1

公开(公告)日：2006-04-27

申请号：US11228455

申请日：2005-09-19

申请人： Robert Thorne ,  Zachary Stum ,  Kevin O'Neill ,  Jan Kmetko

发明人： Robert Thorne ,  Zachary Stum ,  Kevin O'Neill ,  Jan Kmetko

IPC分类号： C30B27/02 ,  C30B15/00 ,  C30B21/06 ,  C30B28/10 ,  C30B30/04

CPC分类号： C30B29/58 ,  C30B35/00 ,  G01N23/20 ,  G01N23/20025 ,  Y10S269/90 ,  Y10S269/908 ,  Y10S269/909 ,  Y10T29/53961 ,  Y10T117/1036

摘要： Sample mounts (10) for mounting microcrystals of biological macromolecules for X-ray crystallography are prepared by using patterned thin polyimide films (12) that have curvature imparted thereto, for example, by being attached to a curved outer surface of a small metal rod (16). The patterned film (12) preferably includes a tapered tip end (24) for holding a crystal. Preferably, a small sample aperture is disposed in the film for reception of the crystal. A second, larger aperture can also be provided that is connected to the sample aperture by a drainage channel, allowing removal of excess liquid and easier manipulation in viscous solutions. The curvature imparted to the film (12) increases the film's rigidity and allows a convenient scoop-like action for retrieving crystals. The polyimide contributes minimally to background and absorption, and can be treated to obtain desired hydrophobicity or hydrophilicity.

摘要翻译： 用于安装用于X射线晶体学的生物大分子的微晶的样品安装件（10）通过使用具有赋予曲率的图案化的聚酰亚胺薄膜（12）来制备，例如通过附接到小金属棒的弯曲外表面 16）。 图案化膜（12）优选地包括用于保持晶体的锥形尖端（24）。 优选地，在膜中设置小的样品孔以接收晶体。 还可以提供第二个更大的孔，其通过排水通道连接到样品孔，允许去除多余的液体并且更容易在粘性溶液中操作。 赋予膜（12）的曲率提高了膜的刚性，并且允许用于取出晶体的方便的勺状作用。 聚酰亚胺对背景和吸收的贡献最小，可以进行处理以获得所需的疏水性或亲水性。