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14 条记录 (耗时 0.03 秒)

    Enzyme immobilization with polysulfonium salts
    1.
    发明授权
    Enzyme immobilization with polysulfonium salts 失效
    用多锍盐固定酶

    公开(公告)号：US4929556A

    公开(公告)日：1990-05-29

    申请号：US216916

    申请日：1988-07-08

    申请人： Roberta C. Cheng ,  Ritchie A. Wessling ,  Donald L. Schmidt

    发明人： Roberta C. Cheng ,  Ritchie A. Wessling ,  Donald L. Schmidt

    IPC分类号： C12N11/02 ,  C12N11/12 ,  C12P19/24

    CPC分类号： C12P19/24 ,  C12N11/02 ,  C12N11/12

    摘要： Polysulfonium salts that can react with nucleophilic groups and covalently cross-link are used to immobilize enzymes or enzyme-containing cellular material. Some of the polysulfonium salts can both flocculate and covalently cross-link. Replacement of the cross-linker, glutaraldehyde, with the polysulfonium salt results in greater retention of enzyme activity during immobilization. Immobilization is carried out by forming a mixture of an enzyme or enzyme-containing cellular material and the polysulfonium salt and subjecting the mixture to conditions such that sulfonium ions react with nucleophilic groups contained by the enzyme or cellular material to form a covalently cross-linked and water insoluble product. The enzyme or cellular material may be flocculated with a flocculating agent prior to cross-linking with the polysulfonium salt. The polysulfonium salt can be a polymer containing sulfonium groups.

    摘要翻译： 可与亲核基团反应并共价交联的聚锍盐用于固定酶或含酶细胞材料。 一些聚锍盐可以絮凝和共价交联。 用聚锍盐替代交联剂戊二醛导致固定期间酶活性的更大保留。 通过形成酶或含酶细胞材料和聚锍盐的混合物进行固定，并使混合物经受使锍离子与酶或细胞材料所含的亲核基团反应以形成共价交联和 水不溶物。 在与多锍盐交联之前，酶或细胞材料可以用絮凝剂絮凝。 聚锍盐可以是含有锍基团的聚合物。

    Stabilization of intracellular enzymes
    4.
    发明授权
    Stabilization of intracellular enzymes 失效
    稳定细胞内酶

    公开(公告)号：US4950596A

    公开(公告)日：1990-08-21

    申请号：US20407

    申请日：1987-03-02

    申请人： Roberta C. Cheng ,  Norman G. Moll ,  Robert A. Houtchens ,  Karen M. McCoy

    发明人： Roberta C. Cheng ,  Norman G. Moll ,  Robert A. Houtchens ,  Karen M. McCoy

    IPC分类号： C12N1/04 ,  C12N9/96 ,  C12N11/02 ,  C12N11/04 ,  C12P19/24

    CPC分类号： C12P19/24 ,  C12N1/04 ,  C12N11/02 ,  C12N11/04 ,  C12N9/96

    摘要： The subject invention concerns a process for stabilizing intact or ruptured microbial cells having glucose isomerase associated therewith. Specifically exemplified is a process for stabilizing glucose isomerase producing cells of a microorganism belonging to the genus Ampullariella. In the invention process the whole or ruptured microbial cells are contacted with a partially carboxyalkylated- or partially phosphonoalkylated-cationic polyelectrolyte, for example, a partially carboxymethylated polyethyleneimine to flocculate and stabilize the cells. The flocculated cells are further stabilized by encapsulation with a partially carboxyalkylated- or partially phosphonoalkylated-cationic polyelectrolyte. The encapsulation can be done prior to or after the flocculated cells are crosslinked. The net effect is manifested by a dramatic increase in the half-life of the glucose isomerase.

    摘要翻译： 本发明涉及用于稳定具有与其相关的葡萄糖异构酶的完整或破裂的微生物细胞的方法。 具体举例说明了稳定葡萄糖异构酶产生细胞的方法，该细胞属于安瓿属（Ampullariella）属。 在本发明方法中，将全部或破裂的微生物细胞与部分羧烷基化或部分膦酰烷基化阳离子聚电解质（例如部分羧甲基化的聚乙烯亚胺）接触以使细胞絮凝和稳定。 通过用部分羧烷基化或部分膦酰烷基化阳离子聚电解质包封进一步稳定絮凝的细胞。 可以在絮凝的细胞交联之前或之后进行封装。 净效应表现在葡萄糖异构酶的半衰期急剧增加。

    Stabilization of glucose isomerase
    10.
    发明授权
    Stabilization of glucose isomerase 失效
    葡萄糖异构酶稳定

    公开(公告)号：US4675292A

    公开(公告)日：1987-06-23

    申请号：US707774

    申请日：1985-03-04

    申请人： Robert A. Houtchens ,  Roberta C. Cheng ,  Karen M. McCoy ,  Carol C. Epstein ,  Norman G. Moll

    发明人： Robert A. Houtchens ,  Roberta C. Cheng ,  Karen M. McCoy ,  Carol C. Epstein ,  Norman G. Moll

    IPC分类号： C12N11/00 ,  C12N9/96 ,  C12N11/04 ,  C12N11/08 ,  C12P19/24

    CPC分类号： C12N11/08 ,  C12N11/04 ,  C12N9/96 ,  C12P19/24

    摘要： Extracellular enzymes are stabilized with a carboxyalkylated or phosphonoalkylated polymer having a molecular weight of at least 500 Daltons. Exemplified is the stabilization of the enzyme glucose isomerase used in a process to convert D-glucose to D-fructose. In a preferred embodiment of the invention the feedstream containing the substrate is contacted initially with the stabilizer and then with the enzyme. In this system the stabilizer and enzyme are maintained in separate reactors. This separation, advantageously, results in a higher half-life for the enzyme.

    摘要翻译： 用分子量至少为500道尔顿的羧基烷基化或膦酰基烷基化聚合物稳定细胞外酶。 示例是用于将D-葡萄糖转化为D-果糖的方法中使用的酶葡萄糖异构酶的稳定化。 在本发明的优选实施方案中，含有底物的进料流最初与稳定剂接触，然后与酶接触。 在该系统中，稳定剂和酶保持在单独的反应器中。 这种分离有利地导致酶的较长的半衰期。