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    Method of Producing Human IgG Antibodies with Enhanced Effector Functions
    2.
    发明申请
    Method of Producing Human IgG Antibodies with Enhanced Effector Functions 审中-公开
    用增强效应功能生产人IgG抗体的方法

    公开(公告)号：US20090215639A1

    公开(公告)日：2009-08-27

    申请号：US11912568

    申请日：2006-04-26

    申请人： Roberto Crea ,  Arvind Rajpal ,  Guido Cappuccilli ,  Toshihiko Takeuchi ,  Ramesh Bhatt ,  Randy Shen

    发明人： Roberto Crea ,  Arvind Rajpal ,  Guido Cappuccilli ,  Toshihiko Takeuchi ,  Ramesh Bhatt ,  Randy Shen

    IPC分类号： C40B30/04 ,  C40B30/00 ,  C12P19/34

    CPC分类号： C07K16/00 ,  C07K2317/72 ,  C07K2319/30 ,  C07K2319/33 ,  C12N15/1051 ,  C12N15/1086

    摘要： A method for generating human IgG1 antibodies with enhanced Fc effector function is disclosed. In practicing the method, an IgG1 Fc look-through mutagenesis (LTM) coding library directed at four receptor-contact regions of the Fc CH2 portion of in human IgG1 Fc is expressed in a system in which the mutated Fc fragments are displayed on the surfaces of the expression cells. The fragments are then screened for altered binding affinity to a selected Fc receptor or other Fc-binding protein. The selected mutations may be used, in turn, to guide the selection of multiple substitutions in the construction of a walk-through mutation (WTM) library, for generating additional Fc fragment mutations with desired binding properties. The antibodies so produced have a variety of therapeutic and diagnostic applications.

    摘要翻译： 公开了产生具有增强的Fc效应子功能的人IgG1抗体的方法。 在实施该方法中，针对人IgG1 Fc中的Fc CH2部分的四个受体 - 接触区域的IgG1 Fc直通诱变（LTM）编码文库在其中突变的Fc片段显示在表面上的系统中表达 的表达细胞。 然后筛选片段以改变与选定的Fc受体或其它Fc结合蛋白的结合亲和力。 可以使用选定的突变来指导在构建穿越突变（WTM）文库中的多个取代的选择，以产生具有期望的结合性质的另外的Fc片段突变。 所产生的抗体具有多种治疗和诊断应用。

    METHOD OF PRODUCING HUMAN IGG ANTIBODIES WITH ENHANCED EFFECTOR FUNCTIONS
    3.
    发明申请
    METHOD OF PRODUCING HUMAN IGG ANTIBODIES WITH ENHANCED EFFECTOR FUNCTIONS 审中-公开
    生产具有增强效应功能的人IGG抗体的方法

    公开(公告)号：US20120107871A1

    公开(公告)日：2012-05-03

    申请号：US13268149

    申请日：2011-10-07

    申请人： Roberto Crea ,  Guido Cappuccilli ,  Toshihiko Takeuchi ,  Arvind Rajpal ,  Ramesh Bhatt ,  Randy Shen

    发明人： Roberto Crea ,  Guido Cappuccilli ,  Toshihiko Takeuchi ,  Arvind Rajpal ,  Ramesh Bhatt ,  Randy Shen

    IPC分类号： C12P21/00 ,  C07H1/00 ,  C07H21/04

    CPC分类号： C07K16/00 ,  C07K2317/72 ,  C07K2319/30 ,  C07K2319/33 ,  C12N15/1051 ,  C12N15/1086

    摘要： A method for generating human IgG1 antibodies with enhanced Fc effector function is disclosed. In practicing the method, an IgG1 Fc look-through mutagenesis (LTM) coding library directed at four receptor-contact regions of the Fc CH2 portion in human IgG1. Fc is expressed in a system in which the mutated Fc fragments are displayed on the surfaces of the expression cells. The fragments are then screened for altered binding affinity to a selected Fc receptor or other Fc-binding protein. The selected mutations may be used, in turn, to guide the selection of multiple substitutions in the construction of a walk-through mutation (WTM) library, for generating additional Fc fragment mutations with desired binding properties. The antibodies so produced have a variety of therapeutic and diagnostic applications.

    摘要翻译： 公开了产生具有增强的Fc效应子功能的人IgG1抗体的方法。 在实施该方法中，针对人IgG1中Fc-CH2部分的四个受体 - 接触区域的IgG1 Fc直通诱变（LTM）编码文库。 Fc在其中突变的Fc片段显示在表达细胞的表面上的系统中表达。 然后筛选片段以改变与选定的Fc受体或其它Fc结合蛋白的结合亲和力。 可以使用选定的突变来指导在构建穿越突变（WTM）文库中的多个取代的选择，以产生具有期望的结合性质的另外的Fc片段突变。 所产生的抗体具有多种治疗和诊断应用。

    Universal Antibody Libraries
    4.
    发明申请
    Universal Antibody Libraries 审中-公开
    通用抗体库

    公开(公告)号：US20110245108A1

    公开(公告)日：2011-10-06

    申请号：US11571710

    申请日：2005-07-06

    申请人： Roberto Crea ,  Arvind Rajpal ,  Guido Cappuccilli ,  Randy Shen ,  Toshihiko Takeuchi

    发明人： Roberto Crea ,  Arvind Rajpal ,  Guido Cappuccilli ,  Randy Shen ,  Toshihiko Takeuchi

    IPC分类号： C40B40/02 ,  C40B40/08 ,  C40B50/06

    CPC分类号： C07K16/00 ,  C07K2317/21 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/622

    摘要： Universal antibody libraries are described which are synthetic and derived from expressed human antibody sequences selected accordingly to certain criteria, for example, that the sequences are derived from naturally-occurring antibodies expressed in response to a certain antigen class (e.g., small molecule, polysaccharide, peptide, or protein) and having CDR regions engineered for optimal diversity. Methods for making and screening such libraries for isolating therapeutics suitable for treating disease are also disclosed.

    摘要翻译： 描述了通用抗体文库，其是合成的，并且衍生自相应于某些标准的表达的人抗体序列，例如，所述序列衍生自响应某一抗原类别（例如小分子，多糖， 肽或蛋白质）并且具有被设计用于最佳多样性的CDR区域。 还公开了用于制备和筛选用于分离适合治疗疾病的治疗剂的文库的方法。